@piatkevich.bsky.social
Gearing up for next year’s teaching at Westlake University. Here’s my intro slide for Super-Resolution Imaging Techniques
November 26, 2025 at 12:00 AM
Gearing up for next year’s teaching at Westlake University. Here’s my intro slide for Super-Resolution Imaging Techniques
Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking www.nature.com/articles/s41...
November 19, 2025 at 12:19 AM
Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking www.nature.com/articles/s41...
In situ imaging proteomics via expansion (iPEX) integrates isotropic tissue magnification with MALDI mass spectrometry imaging to enable unprecedented spatial resolution and depth of protein identification for untargeted spatial proteomics www.nature.com/articles/s41...
iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion - Nature
Isotropic tissue magnification is integrated with matrix-assisted laser desorption/ionization mass spectrometry imaging to enable untargeted spatial proteomics at micrometre resolution and with high p...
www.nature.com
November 13, 2025 at 12:40 AM
In situ imaging proteomics via expansion (iPEX) integrates isotropic tissue magnification with MALDI mass spectrometry imaging to enable unprecedented spatial resolution and depth of protein identification for untargeted spatial proteomics www.nature.com/articles/s41...
Our red calcium indicator, FRCaMPi, and its soma-localized version, SomaFRCaMPi, are now available from @addgene.bsky.social in various vectors, along with all the sensors used for benchmarking. www.addgene.org/browse/artic...
November 12, 2025 at 12:08 AM
Our red calcium indicator, FRCaMPi, and its soma-localized version, SomaFRCaMPi, are now available from @addgene.bsky.social in various vectors, along with all the sensors used for benchmarking. www.addgene.org/browse/artic...
ProteomEx is perhaps the most straightforward and simple approach for spatially resolved targeted proteomics, which requires only off-the-shelf reagents. Now with this step-by-step protocol published in Nature Protocols, anyone can do it www.nature.com/articles/s41...
Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples - Nature Protocols
Filter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combi...
www.nature.com
November 11, 2025 at 10:45 AM
ProteomEx is perhaps the most straightforward and simple approach for spatially resolved targeted proteomics, which requires only off-the-shelf reagents. Now with this step-by-step protocol published in Nature Protocols, anyone can do it www.nature.com/articles/s41...
AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...
November 6, 2025 at 10:15 AM
AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...
🔬 FULL WEBINAR NOW AVAILABLE!
Spatial Biology: Innovations & Applications
Featuring experts: → Xiao Wang → Kiryl D. Piatkevich → Jeffrey Spraggins → Fei Chen
Watch the complete session to discover cutting-edge tech 👇
live.vhall.com/v3/lives/wat...
Spatial Biology: Innovations & Applications
Featuring experts: → Xiao Wang → Kiryl D. Piatkevich → Jeffrey Spraggins → Fei Chen
Watch the complete session to discover cutting-edge tech 👇
live.vhall.com/v3/lives/wat...
October 30, 2025 at 8:49 AM
🔬 FULL WEBINAR NOW AVAILABLE!
Spatial Biology: Innovations & Applications
Featuring experts: → Xiao Wang → Kiryl D. Piatkevich → Jeffrey Spraggins → Fei Chen
Watch the complete session to discover cutting-edge tech 👇
live.vhall.com/v3/lives/wat...
Spatial Biology: Innovations & Applications
Featuring experts: → Xiao Wang → Kiryl D. Piatkevich → Jeffrey Spraggins → Fei Chen
Watch the complete session to discover cutting-edge tech 👇
live.vhall.com/v3/lives/wat...
What an incredible experience hosting a webinar on Spatial Biology: Innovations & Applications with @Science/AAAS!
Missed it? Don't worry—we're dropping the full recording soon!
Here's a sneak peek 👇
Missed it? Don't worry—we're dropping the full recording soon!
Here's a sneak peek 👇
October 30, 2025 at 6:21 AM
What an incredible experience hosting a webinar on Spatial Biology: Innovations & Applications with @Science/AAAS!
Missed it? Don't worry—we're dropping the full recording soon!
Here's a sneak peek 👇
Missed it? Don't worry—we're dropping the full recording soon!
Here's a sneak peek 👇
Great commentary by
@yulonglilab
on our two recent papers introducing red genetically encoded biosensors for calcium and potassium imaging!
🔴 FRCaMPi - Enhanced red calcium sensor 🔴 RGEPOs - First-ever red potassium sensors
📖 doi.org/10.1371/jour...
@yulonglilab
on our two recent papers introducing red genetically encoded biosensors for calcium and potassium imaging!
🔴 FRCaMPi - Enhanced red calcium sensor 🔴 RGEPOs - First-ever red potassium sensors
📖 doi.org/10.1371/jour...
Illuminating calcium and potassium dynamics with red fluorescent sensors
Despite recent advances in green genetically encoded ion indicators, developments in red-shifted indicators have been slower. This Primer explores two recent studies in PLOS Biology that engineer red ...
doi.org
October 27, 2025 at 12:58 AM
Great commentary by
@yulonglilab
on our two recent papers introducing red genetically encoded biosensors for calcium and potassium imaging!
🔴 FRCaMPi - Enhanced red calcium sensor 🔴 RGEPOs - First-ever red potassium sensors
📖 doi.org/10.1371/jour...
@yulonglilab
on our two recent papers introducing red genetically encoded biosensors for calcium and potassium imaging!
🔴 FRCaMPi - Enhanced red calcium sensor 🔴 RGEPOs - First-ever red potassium sensors
📖 doi.org/10.1371/jour...
Spatial biology technologies are rapidly advancing, delivering unprecedented insights into cellular organization and disease pathology. Join our October 29th webinar on "Spatial Biology: Innovations & Applications" with great speakers. Register here for free en.westlake.edu.cn/academics/Sc...
October 24, 2025 at 5:12 AM
Spatial biology technologies are rapidly advancing, delivering unprecedented insights into cellular organization and disease pathology. Join our October 29th webinar on "Spatial Biology: Innovations & Applications" with great speakers. Register here for free en.westlake.edu.cn/academics/Sc...
High-quality AAVs for red genetically encoded calcium indicator FRCaMPi are now available from BrainCase anywhere in the world www.ebraincase.com/products-ser...
October 23, 2025 at 2:02 AM
High-quality AAVs for red genetically encoded calcium indicator FRCaMPi are now available from BrainCase anywhere in the world www.ebraincase.com/products-ser...
#OpenCloning opencloning.org now features WeKwikGene integration—streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations!
September 22, 2025 at 2:37 AM
#OpenCloning opencloning.org now features WeKwikGene integration—streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations!
Fluorescent protein-based ticker tapes (FPTT) record transcriptional histories of multiple promoters on single cellular fibers. Quantitative readout is available at any time point. Cells become biological archives of their own gene expression biorxiv.org/content/10.1...
September 16, 2025 at 10:11 AM
Fluorescent protein-based ticker tapes (FPTT) record transcriptional histories of multiple promoters on single cellular fibers. Quantitative readout is available at any time point. Cells become biological archives of their own gene expression biorxiv.org/content/10.1...
Major milestone for WeKwikGene! wekwikgene.wllsb.edu.cn now features 1,000+ ready-to-ship plasmids with 800+ more plasmids under QC. We've processed 1,000+ requests & distributed 3,000+ plasmids worldwide.
September 15, 2025 at 2:58 AM
Major milestone for WeKwikGene! wekwikgene.wllsb.edu.cn now features 1,000+ ready-to-ship plasmids with 800+ more plasmids under QC. We've processed 1,000+ requests & distributed 3,000+ plasmids worldwide.
Our blue FP Electra2 shows excellent brightness & localization in soil-dwelling amoeba D. discoideum as fusion marker, with performance comparable to the golden standard mTagBFP2, all plasmids for Electra2 are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
September 10, 2025 at 1:59 AM
Our blue FP Electra2 shows excellent brightness & localization in soil-dwelling amoeba D. discoideum as fusion marker, with performance comparable to the golden standard mTagBFP2, all plasmids for Electra2 are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
The hCRAFi-CCR2 sensor reports chemokine release in the mouse visual cortex after LPS challenge using two-photon microscopy www.nature.com/articles/s41.... Plasmids and corresponding test AAVs can be requested from WeKwikGene for free wekwikgene.wllsb.edu.cn/publications...
September 9, 2025 at 12:45 AM
The hCRAFi-CCR2 sensor reports chemokine release in the mouse visual cortex after LPS challenge using two-photon microscopy www.nature.com/articles/s41.... Plasmids and corresponding test AAVs can be requested from WeKwikGene for free wekwikgene.wllsb.edu.cn/publications...
hCRAFi-CCR2 is a new chemokine receptor activation-based fluorescent indicator for monitoring spatiotemporal dynamics of CCR2 ligands, including human/mouse CCL2, and CCL7, as well as mouse CXCL1, in culture and in vivo www.nature.com/articles/s41...
July 25, 2025 at 11:25 AM
hCRAFi-CCR2 is a new chemokine receptor activation-based fluorescent indicator for monitoring spatiotemporal dynamics of CCR2 ligands, including human/mouse CCL2, and CCL7, as well as mouse CXCL1, in culture and in vivo www.nature.com/articles/s41...
PSmOrange3 works well for long-term mitochondria tracking in cultured mammalian cells. Plasmids for PSmOrange3 can be requested for free from @WeKwikGene here wekwikgene.wllsb.edu.cn/publications...
July 4, 2025 at 3:01 AM
PSmOrange3 works well for long-term mitochondria tracking in cultured mammalian cells. Plasmids for PSmOrange3 can be requested for free from @WeKwikGene here wekwikgene.wllsb.edu.cn/publications...
Temporal domain multiplexing enables multiplexed imaging in a single channel with spectrally similar FPs using per-pixel unmixing based on differences in photobleaching rates. To facilitate this approach, we developed new far-red FPs www.biorxiv.org/content/10.1...
July 2, 2025 at 2:11 AM
Temporal domain multiplexing enables multiplexed imaging in a single channel with spectrally similar FPs using per-pixel unmixing based on differences in photobleaching rates. To facilitate this approach, we developed new far-red FPs www.biorxiv.org/content/10.1...
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
June 18, 2025 at 12:16 AM
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
Using mBaoJin derivative, Peng Zou lab engineered highly photostable green eFRET GEVI, named Vega, which exhibits more than 20-fold slower photobleaching than Ace-mNeon2, and allows for long-term voltage imaging in brain slice and in vivo in mice biorxiv.org/content/10.1...
June 10, 2025 at 1:24 AM
Using mBaoJin derivative, Peng Zou lab engineered highly photostable green eFRET GEVI, named Vega, which exhibits more than 20-fold slower photobleaching than Ace-mNeon2, and allows for long-term voltage imaging in brain slice and in vivo in mice biorxiv.org/content/10.1...
Xue Han lab from BU used ElectraOFF to achieve routine cellular-resolution imaging over tens of minutes, occasionally up to eighty minutes, in behaving mice with minimal signal loss across neuron types and brain regions www.biorxiv.org/content/10.1...
June 5, 2025 at 2:06 AM
Xue Han lab from BU used ElectraOFF to achieve routine cellular-resolution imaging over tens of minutes, occasionally up to eighty minutes, in behaving mice with minimal signal loss across neuron types and brain regions www.biorxiv.org/content/10.1...
Hour-long voltage imaging in behaving mice! Capitalizing on the high photostability and brightness of mBaoJin, we developed eFRET GEVIs, named ElectraOFF, which extends reliable single spike detection at cellular resolution beyond one hour biorxiv.org/content/10.1...
June 4, 2025 at 9:17 AM
Hour-long voltage imaging in behaving mice! Capitalizing on the high photostability and brightness of mBaoJin, we developed eFRET GEVIs, named ElectraOFF, which extends reliable single spike detection at cellular resolution beyond one hour biorxiv.org/content/10.1...
Due to its high chemical stability and brightness, mBaoJin is a perfect FP for Expansion Microscopy. Three-dimensional view of expanded HeLa cell coexpressing H2B-mTurquoise2 and Mito (COX8Ax2)-mBaoJin (www.nature.com/articles/s41...)
May 20, 2025 at 1:30 AM
Due to its high chemical stability and brightness, mBaoJin is a perfect FP for Expansion Microscopy. Three-dimensional view of expanded HeLa cell coexpressing H2B-mTurquoise2 and Mito (COX8Ax2)-mBaoJin (www.nature.com/articles/s41...)
Soma-targeting of GECIs significantly improves image segmentation, as can be seen from these examples of single-plane 2-photon imaging for FRCaMPi, jRGECO1a, and their soma-localized variant from L2/3 neurons of primary visual cortex in awake mouse journals.plos.org/plosbiology/...
May 19, 2025 at 12:54 AM
Soma-targeting of GECIs significantly improves image segmentation, as can be seen from these examples of single-plane 2-photon imaging for FRCaMPi, jRGECO1a, and their soma-localized variant from L2/3 neurons of primary visual cortex in awake mouse journals.plos.org/plosbiology/...