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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 1d

Makes $100k/year but can't afford $800/month rent...? Even with child support something doesn't add up.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 1d

Why did this man chose to be a father 4 times, when he clearly never wanted to be involved in taking care of his own kids?

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 3d

I just got my annual #FluShot 💉. Thanks to my employer, St. John's University, for organizing the Flu Clinic on campus every year.

#VaccinesWork
#VaccinesSaveLives

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 4d

#scientistswhocook: spaghetti with fish collar meat from salmon and red snapper, in a while wine, garlic, mascarpone, and parsley sauce.

#spaghetti #salmon #RedSnapper

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 4d

I listened to the article on the Nature podcast, Helen; Very timely. Too bad the scientifically unfounded claim that taking #acetaminophen #Tylenol during pregnancy is linked to autism came out just a few days later.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 6d

I'm grateful that I live in a state where I can still get the #CovidVaccine If I so choose.

Shot number 7 for me 💉 since January 2021.

#VaccinesWork
#VaccinesSaveLives

Matteo Ruggiu, PhD @rnadoctor.bsky.social · 8d

The worst part, apart from the selfish sister, is the mom scolding OP for something she, the mom, is also not willing to do.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 12d

She can trust that he will do the same thing next time. This is the amount of support she will get:

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 12d

Same.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 14d

The husband is a sociopath. However, it sounds like they only dated for 6 months before getting married. Kinda short, I think?

Also, getting pregnant to make him "feel secure"...? What does that even mean?

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 16d

I just downloaded the audiobook 📖

Matteo Ruggiu, PhD @rnadoctor.bsky.social · 18d

He didn't panic. He made a conscious decision. He decided that her medical emergency was less important than his night out. That is not panicking; that is not caring about your partner.

She should dump him and all the friends who are minimizing what happened.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 23d

This is why getting married and having kids when you are 20 is not a great idea.

Also, he resents her for having things easy (from his point of view), and yet it seems that he is not involved in anything kids-related. He should try to take care of the kids and the house just for 1 day.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 23d

🤘 "Nova is the key to include the Z" 🤘

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

She thinks that HE embarrassed her family...? Not her dad, belittling him in front of everyone? Make it make sense.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

This is not a small issue. Having a dog is going to impact their everyday life, freetime, daily schedules, traveling, vacations, visits by friends/family, etc... It's absurd that he just imposed this on her completely disregarding her needs.

He is entitled to have a dog, sure. On his own.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

This was a collaborative project with Alberto Stolfi at GeorgiaTech and @lionlchristiaen.bsky.social at @nyu.edu, @msarscentre.bsky.social, and @unibergen.bsky.social .

Alberto also created a song for this paper, in the style of 80s hard rock. Check it out 🔈

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

Finally, we show that the #transcription factor Ebf activates Nova in Ciona motor neurons — linking transcriptional regulation of neuronal fate to this RNA-based synapse assembly pathway.

Together, our work reveals conserved & divergent features of the Nova–Agrin splicing axis in chordates.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

But we also found surprises:

⚡️ Ciona Nova requires both KH1 + KH2 domains for splicing, with a unique regulatory mechanism involving KH3 and the N/C termini.

⚡️ Mouse Nova proteins cannot splice Ciona Agrin — an example of Developmental System Drift despite overall pathway conservation.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

Using the #tunicate Ciona robusta (the closest relative of vertebrates), we found that this same splicing pathway exists outside vertebrates!

Ciona Nova promotes Agrin Z exon inclusion → Agrin binds Lrp4 → AChRs cluster in muscle.
This pathway likely dates back to the last common chordate ancestor.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

In mammals, clustering of acetylcholine receptors (AChRs) at neuromuscular synapses depends on Agrin splicing by Nova1/2 proteins. Nova ensures the inclusion of tiny “Z” exons, creating a neural-specific Agrin isoform that triggers AChR clustering via Lrp4.

We asked: is this ancient?

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 24d

🚨 New paper alert! 🚨

Our paper entitled: "Neuron-specific Agrin splicing by Nova RNA-binding proteins regulates conserved neuromuscular junction development in chordates" is out now on @plosbiology.org !

journals.plos.org/plosbiology/...

#splicing #microexons #NMJ #Ciona

🧵👇

Neuron-specific Agrin splicing by Nova RNA-binding proteins regulates conserved neuromuscular junction development in chordates

Neuron-specific splicing of Agrin by Nova RNA-binding proteins is essential for neuromuscular synapse formation via acetylcholine receptor clustering. This study shows that this RNA splicing pathway i...

journals.plos.org

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Reposted by Matteo Ruggiu, PhD

PLOS Biology @plosbiology.org · 25d

Neuron-specific splicing of Agrin by Nova is essential for AChR clustering at the NMJ. @rnadoctor.bsky.social @lionlchristiaen.bsky.social &co show that this pathway is conserved in tunicates & that the transcription factor Ebf is a key activator of Nova expression @plosbiology.org 🧪 plos.io/3VhTwig

Top left: Two-color double ISH of Nova (green) and Islet (magenta) showing co-expression in the bilaterally symmetric Motor Neuron 2 (MN2) pair of cells (arrows). Note that Nova mRNA distribution appears more nuclear than Islet in these cells at this stage. Top right: Summary model diagram of a conserved Nova-Agrin-Lrp4 pathway for AChR clustering in Ciona. Upper panel: Summary of conserved pathway, and previously unidentified Ebf-Nova regulatory connection identified in Ciona. Lower panel: Model of proposed mechanism for Ciona Nova-dependent alternative Agrin splicing via binding of KH1 and KH2 domains to two YCAY-rich NISEs identified in the intron 3′ to the Z exons (though not known in what exact orientation or configuration). Inhibitory effect of KH3 domain is relieved by the N- and C-termini. Diagram not drawn to scale. Bottom: Neural-specific CRISPR-mediated disruption of Agrin using sgRNAs results in loss of Acetylcholine receptor A1::GFP clusters (AChRA1::GFP, green) in tail muscles, driven by the Tbx6-r.b promoter. Motor neuron axons labeled by VAChT > Unc-76::mCherry in magenta.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 26d

#scientistswhocook: spaghetti with Acadian redfish in a garlic, white wine, mascarpone, parsley, and olive oil sauce. Paired with a Stein sparkling rosé from the Mosel region in Germany.

#spaghetti #redfish #mascarpone #sparkling #rosé #Mosel #Germany

Spaghetti with Acadian redfish in a garlic, mascarpone, white wine, parsley, and olive oil sauce.

Stein sparkling rosé from the Mosel region in Germany.

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Matteo Ruggiu, PhD @rnadoctor.bsky.social · 28d

It's telling that it didn't even cross his mind to discuss this with her. Like, "I'm going to do this and I don't care what you think".

Also, at least offer to change seats mid flight, so she also gets to enjoy the extra comfort for a while?

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