This #Halloween 🦙🧛‍♂️ Alpacula brewed up #SMLM treat!
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB

I would recommend changing tubing each (sensitive) experiment. In practice, in Exchange-PAINT workflow we change tubing only after multiple experiments. Of course, this requires tubing flushing after each experiment - we use ethanol 80% and then distilled water. It works great!

as for tubing - Tygon is excellent: biocompatible, non-sticky and relatively cheap. We use it successfully for years even with sticky dyes. Of course once in a while we change tubing. Other tubing types should work as well - we did not try.

thanks for your interest! We use low pressure to push the fluid. When we stop the pressure (by closing the valve) the flow stops due to capillary forces. The pressure tubes have to be positioned on the same level as the sample to avoid gravitation-caused flow.

Pumpy is great! In our implementation, we can bring the pressure tubes with fluids close to a sample. Typically, we used tubing length 10-15 cm, ensuring fast response and small dead volumes. Pictures will follow

👏 Huge credit to Samrat Basak @samratb.bsky.social , Kim-Chi Vu, Nikos Mougios, and all authors!
Special thanks to Nazar Oleksiievets, Felipe Opazo @opazo.bsky.social, and Sören Brandenburg for their great support.

Our air-driven system automates Exchange-PAINT workflows — flexible, modular, compatible with most SMLM setups, minimal dead volumes, and ready for smart automation.

Benchmarked with 5-plex imaging in U2OS cells and primary cardiomyocytes.

We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput.

👉 BioRxiv: www.biorxiv.org/content/10.1...

#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing

Check the live stream of the Göttingen Expansion Forum 2025 if you're interested! I think the link changes every day will try to update. Program is here (pdf link): www.rizzoli-lab.de/wp-content/u...

We showcase multiplexed 3D #SMLM imaging of microtubules, vimentin, and actin in U2OS cells 🧬💡

A massive collaborative effort - special thanks to Samrat Basak @samratb.bsky.social for driving the project!

#FLIM #FLPAINT #FLSMLM #MIETPAINT
#DNApaint #MIET #Cytoskeleton

🚨 New preprint update! 🚨
We introduce Confocal MIET-PAINT — extending our 3D nanoscopy method with:
✨ High multiplexing capacity
✨ Isotropic nanometer-scale resolution (lateral + axial)
✨ Compatibility with custom & commercial confocal SMLM equipped with FLIM
www.biorxiv.org/content/10.1...

To anyone who were excited for the release of SUM-PAINT, we now published a detailed protocol on to how replicate it in your hands! Check it out here: doi.org/10.1016/j.xp...

Our invited speaker list is now online and we hope that your are as excited as we are! 🤗

It's a very targeted short review and hopefully is an easy read! Enjoy!! 🙂

Thank you, Amir! Here is the correct link: www.degruyter.com/document/doi...

In this work, we explored wide-field FL-SMLM and took an unconventional approach by combining a review with a detailed imaging protocol. It was particularly exciting to delve into the future prospective of novel wide-field FLIM technologies! 🔬

Thrilled to share our latest review paper, "Advanced Fluorescence Lifetime-Enhanced Multiplexed Nanoscopy of Cells", co-authored with @samratb.bsky.social, now published in Methods in Microscopy! 🚀✨

Check it out here: www.degruyter.com/document/doi... #FLIM #SMLM #FLPAINT #FLSMLM #MIETPAINT