banner
sevaplasmids.bsky.social
@sevaplasmids.bsky.social
A non-profit Open Access repository of standardised plasmids following the SEVA format. Check out our database and request your #plasmid free of charge #synbio

seva-plasmids.com
🧬 Looking for the perfect plasmid?
Explore our #SEVAplasmids database. Request any construct for free, or be the first to design one and share it with the community!
Join the standard 👉 seva-plasmids.com/find-your-pl...
FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…
seva-plasmids.com
November 26, 2025 at 11:01 AM
💡Glutamate from methanol?

This study confirms that MscS channels in Bacillus methanolicus are key L-glutamate exporters, suggesting a way to unlock the bacterium’s potential for sustainable methanol-based glutamate production.

Take a look 👉 doi.org/10.1111/1751...
doi.org
November 24, 2025 at 11:01 AM
🚀 New breakthrough! Researchers from the D-CHAB and the TUM Faculty of Natural Sciences have designed bacterial ABC transporters to efficiently import non-canonical amino acids. This achievement is a significant step towards expanding the genetic code. doi.org/10.1038/s415...
Hijacking a bacterial ABC transporter for genetic code expansion - Nature
Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel…
doi.org
November 21, 2025 at 11:02 AM
🚀 Take your genetic engineering to the next level!
#SEVA plasmids give you a flexible, plug-and-play platform for your #SynBio designs.
Mix, match, and build the plasmid you need, all in one place 👇
seva-plasmids.com/find-your-pl...
FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture
Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…
seva-plasmids.com
November 19, 2025 at 11:02 AM
🤖 GPT goes #SynBio! Researchers at Chinese Academy of Sciences introduce SynBioGPT v2.0, an AI Large Language Model for SynBio that automates knowledge extraction and strain design. Tested on a 100-question SynBio benchmark, it achieved 98% accuracy!
Take a look: doi.org/10.1101/2025...
SynBioGPT: A Retrieval-Augmented Large Language Model Platform for AI-Guided Microbial Strain Development
Synthetic biology seeks to engineer microbial cell factories for sustainable bioproduction, yet the optimization of these systems is impeded by the complexity of metabolic engineering and the…
doi.org
November 17, 2025 at 11:02 AM
🚨 News on bioremediation! A study published in Nature reveals how by genetically modifying the bacterium Vibrio natriegens, it simultaneously degrades five toxic contaminants in industrial wastewater and saline soils. Read the full article: www.nature.com/articles/d41...
Engineered bacteria can degrade five wastewater pollutants at the same time
Five gene clusters have been introduced into a single rapidly growing bacterial strain.
www.nature.com
November 14, 2025 at 11:02 AM
👩🔬 Want to team up with us to expand the #SEVA collection?
Contribute your own constructs to our open plasmid repository and help make #SynBio more reproducible for everyone.
✨ Check out our contribution guidelines and join the mission!👇👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
November 12, 2025 at 11:01 AM
Designing replication sequences is challenging in #SynBio. Researchers at the Innovative Genomics Institute present OriGEN, a language model generating functional plasmids beyond wild type, capturing the complex mechanisms of biological replication.
👉 doi.org/10.1101/2025...
Generating functional plasmid origins with OriGen
While generative artificial intelligence has shown promise for biological design, no computational system has yet created sequences proven capable of replication. Focusing on plasmids as minimal…
doi.org
November 10, 2025 at 11:02 AM
🚀 Meet Constructive Bio! This biotech turns living bacteria into programmable biofactories creating new classes of polymers. At #SEVA, we love seeing initiatives like this driving innovation in #SynBio. Read more 👉 www.nature.com/articles/s41...
Editor’s pick: Constructive Bio - Nature Biotechnology
Each year, Nature Biotechnology highlights companies that received sizeable early-stage funding in the previous year. Constructive Bio is using synthetic E. coli and non-canonical amino acids to…
www.nature.com
November 7, 2025 at 11:01 AM
💭 Imagine #SynBio research where every experiment can be easily replicated and labs collaborate seamlessly. No technical hurdles, just shared progress. That’s our vision at #SEVA! Get your free plasmids and join the movement for #standardisation 👉https://seva-plasmids.com/
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
November 5, 2025 at 11:00 AM
Pseudomonas chlororaphis is a promising biofactory, but hard to engineer. A new piece of work has characterised its endogenous CRISPR/Cas I–F system and built a precise editing & CRISPRi toolkit, enabling efficient genetic manipulation. Take a look 👉 pubs.acs.org/doi/10.1021/...
October 31, 2025 at 11:02 AM
Looking for efficient ways to program intercellular communication? 🧬
Researchers at Micalis Institute present a phagemid toolbox —with #SEVA-based variants— for DNA delivery and next-gen multicellular circuits.
👉 doi.org/10.1101/2025...
An M13 phagemid toolbox for engineering tuneable DNA communication in bacterial consortia
Intercellular communication is essential for distributed genetic circuits operating across cells in multicellular consortia. While diverse signalling molecules have been employed—ranging from quorum…
doi.org
October 29, 2025 at 11:03 AM
The evolution of #SynBio advances with new regulatory toolboxes 🧬
This review explores devices from switches & logic gates to memory modules, powering circuits for bioproduction, therapeutics & biosafety — maybe you’ll find the ideal one for your research 👉 doi.org/10.3389/fsyb...
Frontiers | Genetic circuits in synthetic biology: broadening the toolbox of regulatory devices
Devices sensing inputs and generating outputs are fundamental regulatory units, and as such are the basis of more complex regulatory networks. We provide an ...
doi.org
October 27, 2025 at 11:04 AM
🌊 Marine bacteria like Pseudovibrio hold enormous ecological & biotech potential, still largely unexplored.✨ Discover the new SEVA-based genetic toolbox developed by researchers at the University of Illinois Chicago — opening ways to study their biology & new applications👉 doi.org/10.1128/aem....
Evaluation of vectors for gene expression in Pseudovibrio marine bacteria | Applied and Environmental Microbiology
The genus Pseudovibrio of α-Proteobacteria has consistently been isolated from marine sponges and other marine organisms such as corals and algae. Pseudovibrio bacteria are a source of antibiotics…
doi.org
October 24, 2025 at 10:02 AM
What makes a SEVA plasmid unique?

It’s built around 3 fundamental modules:
1️⃣ Origin of replication
2️⃣ Selection marker
3️⃣ Cargo

With this open standard, you can design predictable and efficient genetic systems in bacteria and yeast.
👉 Learn more here:
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 22, 2025 at 10:02 AM
🧬 How many plasmids can a single bacterium carry?

That’s the question Cholpisit Kiattisewee explores in his review on multi-plasmid systems and their impact on #SynBio. And yes, #SEVA is part of the debate 👀
👉 Read the full article: doi.org/10.1098/rsob...
How many plasmids can bacteria carry? A synthetic biology perspective | Open Biology
Plasmids are pinnacle tools in synthetic biology and other biotechnological applications. They serve as the simplest approach to introduce recombinant DNA, which is then transcribed into RNA that…
doi.org
October 17, 2025 at 10:02 AM
⚠️ Hold on! Don’t scroll!
Still don't know the basic design of a SEVA plasmid?

🧩 Combine modular parts and get your own standard plasmid from @SEVAplasmids for free.

Find your match here 👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 15, 2025 at 10:03 AM
Biology isn’t an exact science — but standardisation helps us get closer to reproducibility and clarity.

That’s why @SEVAplasmids created a universal framework for plasmid vector design. Explore it here 👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 13, 2025 at 10:02 AM
How do you imagine an artificial DNA nucleus? This study presents a synthetic DNA protonucleus that enables localized RNA transcription and condensation into nuclear-like architectures. A novel tool to investigate real nuclear function. Take a look
👉 doi.org/10.1038/s414...
Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus
Nuclear biomolecular condensates are functional sub-compartments within the cell nucleus. Here, the authors develop a synthetic DNA protonucleus that enables RNA transcription and condensation into diverse...
doi.org
October 10, 2025 at 10:03 AM
🚀 #SEVA proves its potential once again!

@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...
Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli
A robust in vivo method for engineering plasmid DNA in E. coli using a triple-selection system that works at any copy number, enabling fast, scalable construction of standardized plasmids for synthetic...
doi.org
October 8, 2025 at 10:02 AM
⏳ Stop wasting hours searching for the perfect plasmid for your experiment.

👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.

🔬 Open, reliable, and ready for your research.

Check it out here 👇
seva-plasmids.com
Home - SEVA plasmids - Standard European Vector Architecture
Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…
seva-plasmids.com
October 6, 2025 at 10:01 AM
📰 A computational analysis reveals 3️⃣ scaling laws of plasmids. Longer plasmids have:

1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes

And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...
Scaling laws of bacterial and archaeal plasmids - Nature Communications
The capacity of a plasmid to express genes is constrained by parameters such as its length and copy number. Here, Maddamsetti et al. present a computational method that enables rapid and accurate…
doi.org
October 3, 2025 at 10:02 AM
🧬🔧X. autotrophicus, despite its metabolic flexibility and biosynthetic potential, has few genetic tools available. Researchers from Northeastern University introduce a new toolbox for this microbe, enabling optimisation of processes like bioremediation👉https://doi.org/10.1021/acssynbio.4c00455
Creating a Genetic Toolbox for the Carbon-Fixing, Nitrogen-Fixing and Dehalogenating Bacterium Xanthobacter autotrophicus
Xanthobacter autotrophicus is a metabolically flexible microorganism with two key features: (1) The organism has adapted to grow on a wide variety of carbon sources including CO2, methanol, formate,…
doi.org
October 1, 2025 at 10:03 AM
📢 A study in Nature Communications presents a new framework that integrates scalable orthogonal amplifiers into standardised biological processes, increasing precision, adaptability, and signal-to-noise ratio in complex genetic circuits. Read the full study here👉 doi.org/10.1038/s414...
A framework for complex signal processing via synthetic biological operational amplifiers - Nature Communications
Engineering genetic circuits to control gene expression in response to complex signal inputs remains a challenge due to limited precision. This study introduces operational amplifiers that decode…
doi.org
September 29, 2025 at 10:02 AM
With #SEVAplasmids you’re just 2 steps away from your ideal plasmid!

1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form

That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/
CONTACT - SEVA plasmids - Standard European Vector Architecture
Order plasmids If you wish to order SEVA plasmids, please fill in the form below. We offer this service pro bono so it is free of charge. Large orders ( > 15 plasmids) may experience shipment delays…
seva-plasmids.com
September 26, 2025 at 10:02 AM