Electrostatic properties of disordered regions control transcription factor search and pioneer activity https://www.biorxiv.org/content/10.1101/2025.03.07.641980v1

Impressions from the LCBM Xmas party.!Happy holidays!!! 🎄🎁🎅🏽

#targetsearch #pioneering #transcriptionfactor #chromatin #singlemoleculeimaging #IDR #biophysics #Sox2 #TF-DNAinteractions

Many thanks to my PhD supervisors, @beatfierz.bsky.social and @davidsuter.bsky.social, for their invaluable guidance and support throughout my journey!

Exciting research update 🎉

How do eukaryotic TFs find target sites on the chromatinized genome? How does DNA state—naked or nucleosomal—shape their search strategy?

We investigated how the charge of disordered regions flanking DBDs influences target search and pioneering activity. Check it out! 👇

Check out our #openaccess article "A Site-Specific Click Chemistry Approach to Di-Ubiquitylate H1 Variants Reveals Position-Dependent Stimulation of the DNA Repair Protein RNF168"
▶️ tinyurl.com/27uxumme
@beatfierz.bsky.social

#SIRT7 is a histone deacetylase with highly specific activity on #chromatin substrates.
We just published mechanism-based #cryoEM structures of #SIRT7 on nucleosomes to understand its activity 👇
www.nature.com/articles/s41...
(1/8) #ChemBio #ChemSky

Want to see how PRC1 writes the Polycomb ubiquitin mark on chromatin, in real-time? Check out our latest preprint, done all by Alexandra Teslenko at ISIC EPFL, a tour-de-force of single-molecule enzymology!

www.biorxiv.org/content/10.1...

A nice collection of state-of-the art lineage tracing methods - our Gene expression memory-based lineage tracing approach is also detailed here:
link.springer.com/protocol/10....

1/ Preprint haiku alert 🤓✍🏻

Cells walk a tightrope—
proteins made and then destroyed,
balance must be kept.

Yet how is this done?
A mystery in the cell,
we set out to ask.

Live imaging helps,
plus models and omics, too—
data tells the tale.

The dance of chromatin—
Remodelers and histones,
Who leads, who follows?
🧵 (1/6)

Always nice to share the ideas together! Many THX to who show interest and ideas in our project 🙏 If interested in TF target search, feel free to contact us 📧 @beatfierz.bsky.social @davidsuter.bsky.social

THX to the EMBL travel grant for me to attend such great conference #EMBLOmics