Using multiple cryo-EM structures, we revealed how the periplasmic chaperone SurA dynamically changes its conformation to deliver substrates to the BAM complex.

Miyazaki et al., 2025, bioRxiv

t.co/g7zkwqiwhz

細菌に必須の膜タンパク質 MurJ と、それを阻害するファージペプチドとの構造解析・第２弾！

ファージ由来の SglPP7 と LysM は、同じ Lipid II フリッパーゼ MurJ を標的にするが、結合様式は異なる。収斂進化のようだ。

Hosoda et al., bioRxiv (2025)

t.co/IcDlImMS2F

The complex between a bacteriophage peptide and the essential membrane protein MurJ — Part II

Both SglPP7 and LysM inhibit MurJ, but through distinct binding modes — a striking case of convergent evolution.

Hosoda et al., bioRxiv (2025)

t.co/IcDlImMkd7

Happy to share our 2025 “Open CNS” achievement! 🎉
Many thanks to my students and collaborators 🙏

Cell Reports: doi.org/10.1016/j.ce...
Nat Commun: doi.org/10.1038/s414...
Sci Adv: doi.org/10.1126/scia...

クテリオファージ由来の小型タンパク質LysMが、Lipid II フリッパーゼMurJの機能を阻害する様子をクライオ電子顕微鏡解析で明らかにしました。

Kohga et al., Sci. Adv. (2025)

t.co/u5ZibBF0kA

Our MurJ/LysM paper has been published in Science Advances.

Kohga et al., Sci. Adv. (2025).

This study elucidates, through cryo-electron microscopy, the mechanism by which the small bacteriophage-derived protein LysM inhibits the lipid II flippase MurJ.

t.co/u5ZibBF0kA

We revealed, through cryo-EM and functional analyses, that the small lipoprotein LptM stabilizes LptD at a late stage of LptDE complex maturation in the outer membrane.

Miyazaki et al., Cell Reports (2025)

www.cell.com/cell-reports...

Just posted on bioRxiv!

We reveal the structure of the MurJ/LysM (JM) complex.
A small phage protein, LysM (37 residues), binds to MurJ like a wedge and freezes its motion.

www.biorxiv.org/content/10.1...

Structural basis of a phosphotransferase system xylose transporter https://www.biorxiv.org/content/10.1101/2025.04.02.646771v1

I made a (quite incomplete) starter pack of Japanese or in-Japan researchers. Self-identify, and I will add you!
日本人/日本にいる研究者のスターターパックを作りました。リプしていただければ追加します！
go.bsky.app/7kMz86x

We captured the process of a substrate protein through a membrane using HS-AFM.
A string-like protein (peptide) is being translocated by a single SecYEG-SecA.

Kanaoka et al., Nature communications (2025)

www.nature.com/articles/s41...

タンパク質が膜透過する様子を高速AFMで捉えました １ユニットのSecYEG-SecAを、紐状のタンパク質（ペプチド）が通過しています

名古屋大学 内橋研、東京理科大学 森研 との共同研究です。

Kanaoka et al., Nature communications (2025)

www.nature.com/articles/s41...

Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM https://www.biorxiv.org/content/10.1101/2025.01.03.631273v1

Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM https://www.biorxiv.org/content/10.1101/2025.01.03.631273v1

doi.org/10.1101/2025...

LptM の機能とLptDEM複合体の構造解析です
bioRxiv にポストしました