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Reposted by Azusa Kage

Azusa Kage @azusakage.bsky.social · May 7

Reposting our microPublication now indexed in PubMed:
We analyzed the movement of the unicellular green alga Chlamydomonas using #DeepLabCut, a deep learning-based tracking tool originally developed for animal behavior studies.
pubmed.ncbi.nlm.nih.gov/40331205/

Original videos: 🧵
#protistsonsky

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Azusa Kage @azusakage.bsky.social · May 7

Here is our set of videos capturing eukaryotic flagellar motion at 200 fps, including 46 live cells and 56 demembranated models of the Chlamydomonas uniflagellate mutant. #protistsonsky
zenodo.org/records/1509...

Data for "Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking"

Data for "Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking," microPublication Biology 10.17912/micropub.biology.00153...

zenodo.org

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Azusa Kage @azusakage.bsky.social · May 7

Reposting our microPublication now indexed in PubMed:
We analyzed the movement of the unicellular green alga Chlamydomonas using #DeepLabCut, a deep learning-based tracking tool originally developed for animal behavior studies.
pubmed.ncbi.nlm.nih.gov/40331205/

Original videos: 🧵
#protistsonsky

1 1 3

Azusa Kage @azusakage.bsky.social · May 5

Spring in northern Japan🌸

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Reposted by Azusa Kage

Azusa Kage @azusakage.bsky.social · Apr 22

Our analysis of the rotational movement of Chlamydomonas uniflagellar mutant using #DeepLabCut has just been published in microPublication Biology!
micropublication.org/journals/bio...

Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking | microPublication

micropublication.org

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Azusa Kage @azusakage.bsky.social · Apr 22

All video data (~100 individuals, both live cells and demembranated models) are available on Zenodo, so feel free to explore and reuse!
zenodo.org/records/1509...
#protistsonsky

Data for "Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking"

Data for "Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking," microPublication Biology 10.17912/micropub.biology.00153...

zenodo.org

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Azusa Kage @azusakage.bsky.social · Apr 22

Our analysis of the rotational movement of Chlamydomonas uniflagellar mutant using #DeepLabCut has just been published in microPublication Biology!
micropublication.org/journals/bio...

Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking | microPublication

micropublication.org

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Reposted by Azusa Kage

Swathi @swath1.bsky.social · Mar 2

Oxygen gradients shape E. coli motility patterns as they run away from O2! - 😍

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Reposted by Azusa Kage

Bungo Akiyoshi @bungoakiyoshi.bsky.social · Feb 1

Asked similar question before but I try again. Anyone knows proteins that localize like this? This protein is present in diplonemids/bodonids/euglenids/Naegleria, which have two flagella, but not in trypanosomatids that have just one flagellum. Potentially interesting for flagella/cilia community?

Bungo Akiyoshi @bungoakiyoshi.bsky.social · Feb 1

The world is full of crazy and sad news, but here is a protein that localizes to only one of the two flagella

Fluorescence microscope image showing a protein that goes to only one flagellum

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Reposted by Azusa Kage

Yoshi Ichikawa @ichikawa-lab.bsky.social · Jan 28

We have posted our new #preprint regarding the track recognition mechanism of dynein-2 that works in #cilia on bioRxiv @biorxivpreprint.bsky.social!!
www.biorxiv.org/content/10.1...
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Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination

Eukaryotic cilia and flagella are thin structures present on the surface of cells, playing vital roles in signaling and cellular motion. Cilia structures rely on intraflagellar transport (IFT), which ...

www.biorxiv.org

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Reposted by Azusa Kage

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Jan 28

Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination https://www.biorxiv.org/content/10.1101/2025.01.27.635025v1

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Azusa Kage @azusakage.bsky.social · Jan 22

Interesting. I’m not very familiar with Alphafold, so I didn’t know that.

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Reposted by Azusa Kage

Kate Michie @kmichie.bsky.social · Jan 21

It’s interesting that all versions of Alphafold distort large extended proteins to ‘globularise’ them. It’s fine for people that understand those helices are ‘bent’ but for non experts it’s confusing. 🫤 🤔 It happens a lot and isn’t well discussed in the community.

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Reposted by Azusa Kage

青山剛@室蘭市長 @aoyama-muroran.bsky.social · Jan 13

【青山たけしSNS】
いつもSNSをチェックいただき
ありがとうございます
Xでの発信をメインとしてますが
Instagramも
2000名近くの方に閲覧いただく
（Xとは若干内容が異なります）
Threadsに加えて新たに
Bluesky bsky.app/profile/aoyama…
をはじめました
今後も室蘭の情報発信を続けます

https://bsky.app/profile/aoyama…

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Azusa Kage @azusakage.bsky.social · Jan 11

追加しました！

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Reposted by Azusa Kage

Yoshi Ichikawa @ichikawa-lab.bsky.social · Jan 8

Proud to be part of this paper published in @naturecomms.bsky.social. Using high-speed AFM, we have visualized the real-time translocation of substrate polypeptide by the single molecule of SecYEG-SecA!!!
Please check this amazing video taken by high-speed AFM!!!
www.nature.com/articles/s41...

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Azusa Kage @azusakage.bsky.social · Jan 1

Happy New Year from the northside of Japan🌅🎍❄️

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Azusa Kage @azusakage.bsky.social · Dec 26

New Year decorations at our lab🎍

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Reposted by Azusa Kage

Kirsty Wan @micromotility.bsky.social · Dec 24

Did you know #diatoms are motile? These tiny glass cells have no cilia or flagella and a rigid body yet can glide on surfaces using a specialised slit in the cell wall called the raphe! What is the functional morphology of the raphe? All revealed in our new preprint
www.biorxiv.org/content/10.1...

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Reposted by Azusa Kage

Micah Dunthorn @micahdunthorn.bsky.social · Dec 20

International Society of Protistologists is funding two symposia at the 16th International Congress of Protistology in Seoul, South Korea. Proposals are due 30 January 2025
#protist #protistsonsky
protistologists.org/programs/fun...

Funding - International Society of Protistologists

Student support, seed money, and more. Got a tip for a program that funds protist research? Contact the Vice President. Society funding: The Holz-Conner Travel Fund provides funding for travel […]

protistologists.org

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Reposted by Azusa Kage

Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · Dec 18

🍾Our small #UExM adventure is only starting! #ProtistsOnSky

We are happy to announce that the Moore Foundation will be funding our efforts in creating an Expansion Microscopy atlas of #Microbial #Eukaryotes with @gautamdey.bsky.social @UNIGE
@embl.org

LINK: www.unige.ch/sciences/chi...

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Reposted by Azusa Kage

Prakash Lab @ Miami @vprakashlab.bsky.social · Dec 16

📢 Please re-share! New Preprint Alert -- in collaboration w/ @prannoysuraneni.bsky.social @coralprof.bsky.social and others @univmiami.bsky.social @miamirosenstiel.bsky.social

FlumeX: A Modular Flume Design for Laboratory-Based Marine Fluid-Substrate Studies @SSRN papers.ssrn.com/sol3/papers....

FlumeX: A Modular Flume Design for Laboratory-Based Marine Fluid-Substrate Studies

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Azusa Kage @azusakage.bsky.social · Dec 16

Joined #NewPISlack 👀

Reposted by Azusa Kage

turingpattern @turingpattern.bsky.social · Dec 13

12月1日から、国立遺伝学研究所の所長として赴任しました近藤滋です。
日本の基礎科学を盛り上げるために、精一杯働く所存です。皆さん、ご協力をお願いいたします。
所長挨拶：
www.nig.ac.jp/nig/ja/about...
学生へのメッセージ：
www.nig.ac.jp/nig/ja/phd-p...

どちらも、楽しく読んでいただけるように書きました。特に、学生へのメッセージの方ができが良いので、まずそちらからお読みいただけますと幸いです。

所長挨拶 | 国立遺伝学研究所

遺伝学の中核拠点として生命システムの解明を目指す先端研究を進めています。また、生命科学の基盤となる研究事業を展開しています。これらの活動により、共同利用・共同研究を推進しています。総合研究大学院大学生命科学研究科遺伝学専攻を併設し、優秀な研究者を世に送り出しています。

www.nig.ac.jp

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Azusa Kage @azusakage.bsky.social · Dec 12

For example, drafting a paper in your native language and using gen AI to translate it, or having gen AI check your English, etc