Takeshi Uenaka
@tuenaka.bsky.social
🧠 Neurologist 🇯🇵 | Postdoc @WernigLab @Stanford 🧫 iPSC-derived models for studying neurodegeneration 🔬 Former @brainresilience.bsky.social | Current @hdfcures.bsky.social Fellow
Reposted by Takeshi Uenaka
New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...
Astrocyte induction of disease-associated microglia is suppressed by acute exposure to fAD neurons in human iPSC triple cultures
Lish et al. present a co-culture system of iPSC-derived human microglia, astrocytes,
and neurons to investigate intercellular communication across different environmental
and genetic contexts. Transcr...
www.cell.com
June 5, 2025 at 12:49 AM
New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...
Reposted by Takeshi Uenaka
Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation
While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...
www.biorxiv.org
April 16, 2025 at 6:13 PM
Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Takeshi Uenaka
Silencing of transgenes during iPSC differentiation is a frequent problem. 🧬🫢
Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪
Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!
Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪
Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!
Losing sleep over #iPSC transgene silencing after differentiation?
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation
While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...
www.biorxiv.org
April 16, 2025 at 4:39 PM
Silencing of transgenes during iPSC differentiation is a frequent problem. 🧬🫢
Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪
Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!
Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪
Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!
Losing sleep over #iPSC transgene silencing after differentiation?
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation
While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...
www.biorxiv.org
April 15, 2025 at 3:37 PM
Losing sleep over #iPSC transgene silencing after differentiation?
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social
Same here — too many times😭
So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.
Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...
Supported by @cziscience.bsky.social