New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...

Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research!

www.biorxiv.org/content/10.1...

Silencing of transgenes during iPSC differentiation is a frequent problem. 🧬🫢

Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪

Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!

Huge thanks to our amazing team:
@kampmann.bsky.social @debkrov.bsky.social @florianmerkle.bsky.social @michael-e-ward.bsky.social
Ernest Arenas, Andrew Bassett, Birgitt Schuele, Leslie Thompson, Bill Skarnes, Marius Wernig labs
Thanks to Margaret Sutherland for support via @cziscience.bsky.social

Losing sleep over #iPSC transgene silencing after differentiation?
Same here — too many times😭

So we built a PiggyBac construct that resists silencing.
Expression stays ON, even after differentiation.

Our #preprint is out👇
🔗 www.biorxiv.org/content/10.1...

Supported by @cziscience.bsky.social