Wildtype One
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Researchers,
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
January 27, 2026 at 2:31 PM
Researchers,
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
Remember: Peer review is a human process, and some comments can harm your work 🔬
Wildtype One looked at true stories from researchers
We found 10 reviewer comments you should absolutely ignore
Read today's Wildtype Weekly to find them out 👇
Wildtype One looked at true stories from researchers
We found 10 reviewer comments you should absolutely ignore
Read today's Wildtype Weekly to find them out 👇
Ignore these 10 reviewer comments
“What reviewers should be doing is judging whether the conclusions follow from the premises—not whether they would have done the work differently.” — Sydney Brenner, Nobel Prize Laureate Dear Research...
www.linkedin.com
January 26, 2026 at 12:26 PM
Remember: Peer review is a human process, and some comments can harm your work 🔬
Wildtype One looked at true stories from researchers
We found 10 reviewer comments you should absolutely ignore
Read today's Wildtype Weekly to find them out 👇
Wildtype One looked at true stories from researchers
We found 10 reviewer comments you should absolutely ignore
Read today's Wildtype Weekly to find them out 👇
Quick tip 🔬🧑🔬
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
January 25, 2026 at 7:59 PM
Quick tip 🔬🧑🔬
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
Do you use SEM because it looks cleaner?
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
January 21, 2026 at 3:43 PM
Do you use SEM because it looks cleaner?
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
Many cell lines in research aren't what researchers think they are 🧫
So, how would a world-class scientist troubleshoot their project?
Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)
So, how would a world-class scientist troubleshoot their project?
Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)
In case you chose the wrong cell line…
“Ignorance more frequently begets confidence than does knowledge.” — Charles Darwin Dear Researcher, Could your cell line be the wrong one for your project? Dr.
www.linkedin.com
January 19, 2026 at 3:36 PM
Many cell lines in research aren't what researchers think they are 🧫
So, how would a world-class scientist troubleshoot their project?
Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)
So, how would a world-class scientist troubleshoot their project?
Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)
Researchers,
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
January 18, 2026 at 6:02 PM
Researchers,
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
Sadly, only 11-25% of the biology literature is reproducible
But there's a solution...
(a thread)
But there's a solution...
(a thread)
January 16, 2026 at 2:52 PM
Sadly, only 11-25% of the biology literature is reproducible
But there's a solution...
(a thread)
But there's a solution...
(a thread)
Reviewers will ask you to repeat experiments...
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
January 14, 2026 at 10:56 AM
Reviewers will ask you to repeat experiments...
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
Could your cell line be the wrong one for your disease? 🧫
(a thread)
(a thread)
January 13, 2026 at 2:43 PM
Could your cell line be the wrong one for your disease? 🧫
(a thread)
(a thread)
“There are 5 rules. People tried to bend them, and the data punished them for it.” 🫣
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
What are his rules?
Discover them in today's Wildtype Weekly (in 3 minutes or less 👇)
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
What are his rules?
Discover them in today's Wildtype Weekly (in 3 minutes or less 👇)
A decade’s advice on flow cytometry controls
“A result without an appropriate control is only an anecdote, not an experiment.” — Modern methodological paraphrase Dear Researcher, In last week’s edition, Wildtype One asked Juliano Freitas, Ph.
www.linkedin.com
January 12, 2026 at 8:15 AM
“There are 5 rules. People tried to bend them, and the data punished them for it.” 🫣
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
What are his rules?
Discover them in today's Wildtype Weekly (in 3 minutes or less 👇)
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
What are his rules?
Discover them in today's Wildtype Weekly (in 3 minutes or less 👇)
Everyone’s thinking it. Few say it out loud.
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
January 11, 2026 at 9:17 AM
Everyone’s thinking it. Few say it out loud.
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
How to choose the ideal figure type to your experiment 🥼🔬:
(a thread)
(a thread)
January 10, 2026 at 2:52 PM
How to choose the ideal figure type to your experiment 🥼🔬:
(a thread)
(a thread)
How can you publish in high-impact journals? Here's advice from a Nature Editor
(a thread)
(a thread)
January 8, 2026 at 11:14 AM
How can you publish in high-impact journals? Here's advice from a Nature Editor
(a thread)
(a thread)
So, should you switch to spectral cytometry?
Not before you know a few basic principles.
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
Read what he'd advise you in today's Wildtype Weekly (in 3 minutes or less 👇)
Not before you know a few basic principles.
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
Read what he'd advise you in today's Wildtype Weekly (in 3 minutes or less 👇)
3 questions to ask before switching flow cytometers
“Premature optimization is the root of all evil.” — Donald Knuth Dear Researcher, Happy New Year! If you use conventional flow cytometry, switching to spectral means: Less spillover Better unmixing Im...
www.linkedin.com
January 5, 2026 at 4:19 PM
So, should you switch to spectral cytometry?
Not before you know a few basic principles.
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
Read what he'd advise you in today's Wildtype Weekly (in 3 minutes or less 👇)
Not before you know a few basic principles.
Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice.
Read what he'd advise you in today's Wildtype Weekly (in 3 minutes or less 👇)
Researchers, how would you handle your "non-significant" statistics? 🧫📊
Vote here 👉 www.linkedin.com/feed/update/...
Vote here 👉 www.linkedin.com/feed/update/...
January 4, 2026 at 12:54 PM
Researchers, how would you handle your "non-significant" statistics? 🧫📊
Vote here 👉 www.linkedin.com/feed/update/...
Vote here 👉 www.linkedin.com/feed/update/...
The top 5 Western blots failures researchers complained about online in 2025 🧻
(a thread)
(a thread)
January 1, 2026 at 5:56 PM
The top 5 Western blots failures researchers complained about online in 2025 🧻
(a thread)
(a thread)
Your Western blot shouldn’t take two days
(a thread)
(a thread)
December 31, 2025 at 7:02 AM
Your Western blot shouldn’t take two days
(a thread)
(a thread)
We're researchers. We're paid to think and discover—not solve technical issues.
To make 2026 more productive, we made a list of affordable lab tools that solve the most annoying problems (for less than 1,000€)
Read the last Wildtype One weekly of 2025 🍾 👇
To make 2026 more productive, we made a list of affordable lab tools that solve the most annoying problems (for less than 1,000€)
Read the last Wildtype One weekly of 2025 🍾 👇
Cheap tools that solve annoying lab problems
“There’s a way to do it better—find it.” — Thomas Edison Dear Researcher, We spend a lot of our lab time solving technical issues But we’re scientists We’re paid to think and discover.
www.linkedin.com
December 29, 2025 at 4:19 PM
We're researchers. We're paid to think and discover—not solve technical issues.
To make 2026 more productive, we made a list of affordable lab tools that solve the most annoying problems (for less than 1,000€)
Read the last Wildtype One weekly of 2025 🍾 👇
To make 2026 more productive, we made a list of affordable lab tools that solve the most annoying problems (for less than 1,000€)
Read the last Wildtype One weekly of 2025 🍾 👇
What if a statistician ran a cancer cell-biology lab? 🧫 🧬 (...and what can we learn from them?)
(a thread)
(a thread)
December 27, 2025 at 7:20 PM
What if a statistician ran a cancer cell-biology lab? 🧫 🧬 (...and what can we learn from them?)
(a thread)
(a thread)
Researchers,
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
December 22, 2025 at 4:49 PM
Researchers,
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
Hot take: 96-well plates are NOT inherently high throughput 🧫
(a thread)
(a thread)
December 20, 2025 at 5:23 PM
Hot take: 96-well plates are NOT inherently high throughput 🧫
(a thread)
(a thread)
If you're a young researcher, there is still a lot to learn about flow cytometry 🤕
Today, we decided save you some of the trial and error
We compiled 7 veteran flow cytometry tips (that beginners learn the hard way)
Read today's Wildtype Weekly (in 3 minutes or less)👇
Today, we decided save you some of the trial and error
We compiled 7 veteran flow cytometry tips (that beginners learn the hard way)
Read today's Wildtype Weekly (in 3 minutes or less)👇
Veteran flow cytometry rules (that beginners learn the hard way)
“An experiment is only as good as the controls that accompany it.” — Claude Bernard Dear Researcher, What do urinals 🚽 and flow cytometry 🌈 have in common? Flow cytometry isn’t just “run and gate” The...
www.linkedin.com
December 17, 2025 at 11:03 AM
If you're a young researcher, there is still a lot to learn about flow cytometry 🤕
Today, we decided save you some of the trial and error
We compiled 7 veteran flow cytometry tips (that beginners learn the hard way)
Read today's Wildtype Weekly (in 3 minutes or less)👇
Today, we decided save you some of the trial and error
We compiled 7 veteran flow cytometry tips (that beginners learn the hard way)
Read today's Wildtype Weekly (in 3 minutes or less)👇
This is so important, it's worth repeating:
Isotype control isn't your best negative control in flow cytometry
(a thread)
Isotype control isn't your best negative control in flow cytometry
(a thread)
December 16, 2025 at 1:09 PM
This is so important, it's worth repeating:
Isotype control isn't your best negative control in flow cytometry
(a thread)
Isotype control isn't your best negative control in flow cytometry
(a thread)
Not all articles reproduce. How do you make sure yours does? Read today's Wildtype Weekly (3 minutes or less 👇)
Cancer research is ~20% reproducible (7 steps to protect your data)
Dear Researcher, When we read studies, we assume they should be taken at face value and can be trusted Yet not all data are equal (..
www.linkedin.com
December 8, 2025 at 1:58 PM
Not all articles reproduce. How do you make sure yours does? Read today's Wildtype Weekly (3 minutes or less 👇)
"The reason I quit science was because of an antibody not working!"
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
December 7, 2025 at 7:43 PM
"The reason I quit science was because of an antibody not working!"
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)