Wildtype One
@wildtypeone.bsky.social
Lab content for researchers 🧫 | Join 900+ researchers getting weekly lab hacks with our newsletter (it’s free) 👉 wildtypeone.substack.com/about
All of the following do NOT count as sufficient controls (by lab technique)
(a thread)
(a thread)
February 11, 2026 at 6:29 PM
All of the following do NOT count as sufficient controls (by lab technique)
(a thread)
(a thread)
A tip from over a decade of flow cytometry experience:
(a thread)
(a thread)
February 10, 2026 at 4:25 PM
A tip from over a decade of flow cytometry experience:
(a thread)
(a thread)
Researchers,
Flow data feels objective, until two people gate it
(1/4)
Flow data feels objective, until two people gate it
(1/4)
February 7, 2026 at 10:42 AM
Researchers,
Flow data feels objective, until two people gate it
(1/4)
Flow data feels objective, until two people gate it
(1/4)
FlowJo should not be the default! 🙅♂️
How can you choose the best flow cytometry software for your case?
Here's your rule of thumb:
(1/9)
How can you choose the best flow cytometry software for your case?
Here's your rule of thumb:
(1/9)
January 30, 2026 at 9:57 AM
FlowJo should not be the default! 🙅♂️
How can you choose the best flow cytometry software for your case?
Here's your rule of thumb:
(1/9)
How can you choose the best flow cytometry software for your case?
Here's your rule of thumb:
(1/9)
Researchers, don't choose the default just because everyone uses it. Here's how to pick the ideal flow software for your case (see today's post on LinkedIn 👇)
www.linkedin.com/posts/wildty...
www.linkedin.com/posts/wildty...
January 28, 2026 at 4:09 PM
Researchers, don't choose the default just because everyone uses it. Here's how to pick the ideal flow software for your case (see today's post on LinkedIn 👇)
www.linkedin.com/posts/wildty...
www.linkedin.com/posts/wildty...
Researchers,
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
January 27, 2026 at 2:31 PM
Researchers,
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change
(1/3)
Quick tip 🔬🧑🔬
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
January 25, 2026 at 7:59 PM
Quick tip 🔬🧑🔬
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
If you do Western blots, immunofluorescence, or any technique that requires antibody staining
Remember:
Overnight incubation at 4°C is not always the best protocol ⚠️
(1/2)
Do you use SEM because it looks cleaner?
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
January 21, 2026 at 3:43 PM
Do you use SEM because it looks cleaner?
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
SEM vs. SD = one of the most quietly damaging figure-making habits in biology
Let’s clear it up
(a thread)
Researchers,
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
January 18, 2026 at 6:02 PM
Researchers,
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
Most irreproducible results don’t fail loudly
They quietly rot
(a thread)
Sadly, only 11-25% of the biology literature is reproducible
But there's a solution...
(a thread)
But there's a solution...
(a thread)
January 16, 2026 at 2:52 PM
Sadly, only 11-25% of the biology literature is reproducible
But there's a solution...
(a thread)
But there's a solution...
(a thread)
Reviewers will ask you to repeat experiments...
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
January 14, 2026 at 10:56 AM
Reviewers will ask you to repeat experiments...
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
Only because of small details that you didn't include
How do you avoid losing time and repeating experiments? 🤔
(a thread)
Could your cell line be the wrong one for your disease? 🧫
(a thread)
(a thread)
January 13, 2026 at 2:43 PM
Could your cell line be the wrong one for your disease? 🧫
(a thread)
(a thread)
Everyone’s thinking it. Few say it out loud.
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
January 11, 2026 at 9:17 AM
Everyone’s thinking it. Few say it out loud.
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
The literature looks confident
- Clean figures
- Tight conclusions
- Cropped blots that “say enough”
(1/n)
How to choose the ideal figure type to your experiment 🥼🔬:
(a thread)
(a thread)
January 10, 2026 at 2:52 PM
How to choose the ideal figure type to your experiment 🥼🔬:
(a thread)
(a thread)
How can you publish in high-impact journals? Here's advice from a Nature Editor
(a thread)
(a thread)
January 8, 2026 at 11:14 AM
How can you publish in high-impact journals? Here's advice from a Nature Editor
(a thread)
(a thread)
Researchers, how would you handle your "non-significant" statistics? 🧫📊
Vote here 👉 www.linkedin.com/feed/update/...
Vote here 👉 www.linkedin.com/feed/update/...
January 4, 2026 at 12:54 PM
Researchers, how would you handle your "non-significant" statistics? 🧫📊
Vote here 👉 www.linkedin.com/feed/update/...
Vote here 👉 www.linkedin.com/feed/update/...
The top 5 Western blots failures researchers complained about online in 2025 🧻
(a thread)
(a thread)
January 1, 2026 at 5:56 PM
The top 5 Western blots failures researchers complained about online in 2025 🧻
(a thread)
(a thread)
Your Western blot shouldn’t take two days
(a thread)
(a thread)
December 31, 2025 at 7:02 AM
Your Western blot shouldn’t take two days
(a thread)
(a thread)
What if a statistician ran a cancer cell-biology lab? 🧫 🧬 (...and what can we learn from them?)
(a thread)
(a thread)
December 27, 2025 at 7:20 PM
What if a statistician ran a cancer cell-biology lab? 🧫 🧬 (...and what can we learn from them?)
(a thread)
(a thread)
Researchers,
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
December 22, 2025 at 4:49 PM
Researchers,
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
T-tests are often misused—here’s a quick rule of thumb 👇
(a thread)
Hot take: 96-well plates are NOT inherently high throughput 🧫
(a thread)
(a thread)
December 20, 2025 at 5:23 PM
Hot take: 96-well plates are NOT inherently high throughput 🧫
(a thread)
(a thread)
Seeing the results...
December 16, 2025 at 1:14 PM
Seeing the results...
This is so important, it's worth repeating:
Isotype control isn't your best negative control in flow cytometry
(a thread)
Isotype control isn't your best negative control in flow cytometry
(a thread)
December 16, 2025 at 1:09 PM
This is so important, it's worth repeating:
Isotype control isn't your best negative control in flow cytometry
(a thread)
Isotype control isn't your best negative control in flow cytometry
(a thread)
"The reason I quit science was because of an antibody not working!"
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
December 7, 2025 at 7:43 PM
"The reason I quit science was because of an antibody not working!"
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story
She had a colleague who was working with an antibody manufacturer
Here's the story she shared
(a thread)
December 6, 2025 at 10:57 AM