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Zebrafish Rock! @zebrafishrock.bsky.social · 13h

ICYMI... We are back on Bluesky and LinkedIn with #scicomm posts about your favorite model organism. And look out for #sciart every Sunday as #ZebrafishZunday continues! #RockOn

Zebrafish Rock! @zebrafishrock.bsky.social · 17h

Published October 25 years ago, “Effective targeted gene ‘knockdown’ in zebrafish” by Nasevicius & Ekker detail use of antisense morpholino-modified oligonucelotides or #morpholinos that inhibit translation. This landmark spurred a revolution in targeted gene knockdown in vivo. #ZebrafishFunFacts 🧪

Structures of DNA and morpholino oligonucleotides. R and R' denote continuation of the oligomer chain in the 5' or 3' direction, respectively. Credit to Corey & Abrams (2001) Genome Biology.

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Zebrafish Rock! @zebrafishrock.bsky.social · 17h

Link to paper: (nature.com/articles/ng1...)

Credit to @scekker.bsky.social 🤠

Effective targeted gene ‘knockdown’ in zebrafish - Nature Genetics

Nature Genetics - Effective targeted gene ‘knockdown’ in zebrafish

nature.com

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Zebrafish Rock! @zebrafishrock.bsky.social · 17h

Published October 25 years ago, “Effective targeted gene ‘knockdown’ in zebrafish” by Nasevicius & Ekker detail use of antisense morpholino-modified oligonucelotides or #morpholinos that inhibit translation. This landmark spurred a revolution in targeted gene knockdown in vivo. #ZebrafishFunFacts 🧪

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Zebrafish Rock! @zebrafishrock.bsky.social · 2d

Looking to help a vibrant community? Follow @askzebrafish.bsky.social and help answer some of the community’s most pressing questions/queries! #AskZebrafish

Ask Zebrafish? @askzebrafish.bsky.social · 2d

Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish

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Zebrafish Rock! @zebrafishrock.bsky.social · 2d

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Link to Spence et al: onlinelibrary.wiley.com/doi/10.1111/...

Link to Parichy: elifesciences.org/articles/05635

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Zebrafish Rock! @zebrafishrock.bsky.social · 2d

#ZebrafishFunFacts: Cited more than 1500 times, 'The behaviour and ecology of the zebrafish, Danio rerio' by Spence et al. (2007) highlights the special abilities of a small cyprinid found on the Indian subcontinent. A more recent take by Parichy (2015) expands on this in context of evolution. 1/2 🧪

Zebrafish and their geographic range.
(A) Historic and more recent sites where zebrafish have been reported in India, Nepal, Bangladesh and possibly Myanmar (Spence et al., 2006; Engeszer et al., 2007b; Spence et al., 2008; Whiteley et al., 2011; Arunachalam et al., 2013). (B) Zebrafish from several populations in northeastern India (Engeszer et al., 2007b). The upper two fish are males and the lower two fish are females; males tend to have a slightly yellow cast ventrally. (C) A group of zebrafish (a single fish is highlighted with the arrow) in a stream-side pool in Meghalaya, India, north of Bangladesh. Scale bar: 5 mm (B). Image and text credits: D Parichy.

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Zebrafish Rock! @zebrafishrock.bsky.social · 6d

Cell nuclei movements (color-coded for direction) during early zebrafish development. Credit to Dr. Philipp Keller @hhmijanelia.bsky.social. #ZebrafishZunday

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Zebrafish Rock! @zebrafishrock.bsky.social · 6d

Vasculature in the blood-brain-barrier of a zebrafish larva. Credit to @natashaobrown.bsky.social. #ZebrafishZunday 🧪

Dorsal view of a zebrafish larva with blood vessels in magenta. Anterior to left.

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Reposted by Zebrafish Rock!

Kevin Thiessen, PhD @kevinthiessen.bsky.social · 7d

I'm treading lightly with this one but the science communication bit of @zebrafishrock.bsky.social is back for the foreseeable future. I'll be sharing my plethora of fun facts (including some new ones) with the community. Enjoy!

Zebrafish Rock! @zebrafishrock.bsky.social · 7d

#ZebrafishFunFacts: As the most-cited #zebrafish cancer research paper, Amsterdam et al screened hundreds of heterozygous lines for embryonic lethal mutations & found elevated cancer incidence in ribosomal genes. This previously underappreciated finding was published @plosbiology.org 21 years ago. 🧪

Figure 1. Spindle Cell Tumors Resembling MPNSTs in Zebrafish Heterozygous for Mutations in RP Genes
(A and B) Fish with apparent masses, as indicated by the arrows, or other evident pathology were selected for histological analysis: (A) a hi2582 fish, (B) a hi1034B fish. (C–H) Histopathology of representative tumors stained with hematoxylin and eosin reveals patterns consistent with the diagnosis of MPNST in hi10 fish (C and D), hi1974 fish (E–G), and hi1807 fish (H). (D) A large tumor with central necrosis is seen emanating from the optic nerve (n) (e, eye) (20×).

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Zebrafish Rock! @zebrafishrock.bsky.social · 7d

🔗 to paper: journals.plos.org/plosbiology/...

Many Ribosomal Protein Genes Are Cancer Genes in Zebrafish

A screen for tumour suppressor genes in zebrafish has identified several genes encoding ribosomal proteins, indicating that these genes might be an unappreciated class of cancer genes.

journals.plos.org

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Zebrafish Rock! @zebrafishrock.bsky.social · 7d

#ZebrafishFunFacts: As the most-cited #zebrafish cancer research paper, Amsterdam et al screened hundreds of heterozygous lines for embryonic lethal mutations & found elevated cancer incidence in ribosomal genes. This previously underappreciated finding was published @plosbiology.org 21 years ago. 🧪

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Zebrafish Rock! @zebrafishrock.bsky.social · 9d

Not to get your hopes up but we are posting again… This time we are asking questions from our Slack group using @askzebrafish.bsky.social. Follow, interact, share or answer to your heart’s content and help the fabulous #zebrafish community. You rock! 🧪

Ask Zebrafish? @askzebrafish.bsky.social · 9d

🤔 #AskZebrafish: Looking for Prom1a and/or Prom1b mutant lines and/or reporter lines that someone would be willing to share? Preferably in the USA.

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Zebrafish Rock! @zebrafishrock.bsky.social · 21d

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Zebrafish Rock! @zebrafishrock.bsky.social · 24d

Have you heard of the Zebrafish Rock! Slack Pro tankspace? Sponsored by ZFIN, it acts as the premier source for questions and queries for the #zebrafish community and beyond! If you are keen to join, please fill out this Google form at our website: linktr.ee/zebrafishrock

Zebrafish Rock! | Linktree

Award-winning home tank for all things zebrafish!

linktr.ee

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Zebrafish Rock! @zebrafishrock.bsky.social · Sep 10

De-lurking to promote this neat piece of #zebrafish memorabilia from Rosie Lavis! Now available in its 3rd-edition, this linocut is sure to impress (especially with blue embellishments, if you fancy). Give as a gift or reserve one for yourself. Get yours at www.thewildprintstudio.com/shop/p/zebra...

Hand holding linocut print of a blue and white striped zebrafish in front of more, similar prints.

Zebrafish linocut with blue embellishments. You can note them on the stripes and the tail. It looks even better in person.

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 29

A parting gift to stay connected ❤️ 🧪

Starter Packs for #zebrafish Community:

ZF Resources: go.bsky.app/JcC5df3
#WomenInScience V1: go.bsky.app/4BegEjv
#WomenInScience V2: go.bsky.app/VoebYrt
ZF PIs V1: go.bsky.app/2cHYybY
ZF PIs V2: go.bsky.app/4zBuxAF
ZF PIs V3: go.bsky.app/JvjuSZg

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 26

FYI

Kevin Thiessen, PhD @kevinthiessen.bsky.social · Jun 26

After 7 years and a lot of memories, I've finally decided to retire Zebrafish Rock! on social media. I really want to say it was always positive but I've had a tumultuous experience with the account. Those that make the community more toxic continue to be promoted to positions of power. 1/4

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 25

For those interested! #ZebrafishMeeting

balciunaslab.bsky.social @balciunaslab.bsky.social · Jun 25

We are organizing a knock-in-focused workshop at Vilnius University Life Sciences Center. We would love to put together a session with graduate students and postdocs presenting! Preliminary program has links to registration and getting around. www.gmc.vu.lt/en/events-an...

Genome Editing in Zebrafish: Knock-ins and their applications

VU Gyvybės mokslų centras jungia tris VU padalinius – Biomokslų, Biotechnologijos ir Biochemijos institutus. Centras įkurtas vystant integruotą mokslo, studijų ir verslo centrą (slėnį) „Saulėtekis“.

www.gmc.vu.lt

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 24

Intriguing 🤔 but have you ever seen a fish with trainers on?

a man in a leather jacket is making a funny face while looking at the camera .

Alt: a man in a leather jacket is making a funny face while looking at the camera .

media.tenor.com

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 24

ICYMI from @scekker.bsky.social et al.

Communications Biology @commsbio.nature.com · Jun 24

🐟This recent review emphasises the advantages of using #zebrafish in research, outlines key differences in experimental approaches, & offers guidelines for designing studies in a way that enhances experimental rigor and reproducibility #3Rs doi.org/10.1038/s420...

Zebrafishology, study design guidelines for rigorous and reproducible data using zebrafish - Communications Biology

A review emphasizes the advantages of using zebrafish in research, outlines key differences in experimental approaches, and offers guidelines for designing studies in a way that enhances experimental ...

doi.org

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 24

We already have (Cre-)lox fish, that good enough? 😋

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 24

🔗 to manuscript: journals.biologists.com/dev/article/...

Hubrecht Institute @hubrechtinstitute.bsky.social · Jun 24

The Den Hertog group uncovered a surprising mechanism in zebrafish tail development: when cells from Kupffer’s vesicle fail to migrate, a second tail-like structure can form. The discovery emerged during a screen of fungal metabolites. www.hubrecht.eu/tail-develop... @dev-journal.bsky.social

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 23

And 4 out of 5 all part of the #zebrafish community!

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 23

Peng Huang @penghuang031.bsky.social · Jun 23

Excited to share our latest work led by my PhD student Linjun Xie on sclerotome development: Dynamic BMP signaling regulates sclerotome induction and lineage diversification in zebrafish, now published on @dev-journal.bsky.social! #zebrafish #devbio 1/8 doi.org/10.1242/dev....

Dynamic BMP signaling regulates sclerotome induction and lineage diversification in zebrafish

Summary: Loss- and gain-of-function manipulations reveal that dynamic regulation of BMP signaling is essential for the induction and diversification of the sclerotome lineage in zebrafish.

doi.org

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Zebrafish Rock! @zebrafishrock.bsky.social · Jun 23

Bethanie Carney Almroth #PlasticsTreaty @bcarneyalmroth.bsky.social · Jun 23

📢 NEW PAPER on #Chemicals in #RecycledPlastics! 📢

We collected recycled polyethylene (PE) pellets from small scale mechanical #recycling facilities, measured chemicals leaching out of the plastics into water and impacts in zebrafish.
#PlasticsTreaty #INC5-2

www.sciencedirect.com/science/arti...

Effects of leachates from black recycled polyethylene plastics on mRNA expression of genes involved in adipogenesis and endocrine pathways in zebrafish embryos

Black recycled polyethylene pellets were collected from India, Nigeria and Taiwan. These pellet samples were used to create water leachates (in zebraf…

www.sciencedirect.com