New 105 MP Sony IMX927 sensor (global shutter) capable of streaming data at 100 fps... 10.5 gigavoxels/sec.

www.sony-semicon.com/en/products/...

Latest imaging data from our completely re-designed ExA-SPIM system.

Expansion-assisted imaging (3X expansion, 0.3 NA) enables brain-wide imaging with high-contrast and <400 nm effective resolution.

That is a good data point to know. We hypothesize (no data yet) that aberrations will reduce quickly with expansion factor (RI gradients proportional to density, density gradients will reduce with expansion factor ^3). Whereas the imaging path length only increases linearly with expansion factor.

ExA-SPIM2 will provide 35 mm WD with 0.5 NA.

I think there are definitely applications where higher NA is needed. If we designed a 3rd variant, I think aiming for ~0.8 - 0.85 NA (which allows for symmetric 90 deg objectives) with 20 mm WD would be very valuable.

"Expansion-assisted" is all about balancing light collection (NA^2) with practicality of engineering lenses with both high NA and long WD. We are collecting data now to quantify this, but we also believe aberrations in ExM will scale with NA^3.

So IMO go as low in NA as specimen brightness allows!

First 3200 MP images from the LSST at the Vera C. Rubin Observatory!

www.nature.com/articles/d41...

High-throughput line scanning confocal microscopy
www.biorxiv.org/content/10.1...

None of the above captures the long WD (35 mm). Perhaps we should introduce a volumetric SBP... that divides the usable WD by either the Rayleigh axial resolution, or an isotropic resolution assumption that divides by the lateral resolution -> would be very high for this lens.

Thanks for resolving the numbers.

Agreed, this was the primary motivation for ExA-SPIM in the first place. More pixel parallelization with a larger SBP, either in 2D (global shutter) or even along 1D for rolling shutter (i.e. for ASLM). Also removes some scanning bottlenecks with a low FPS sensor.

That is right, I used the equation from doi: 10.1117/1.ap.3.4.044001. The required number of samples to fully resolve the signal - also their equation uses 0.5 instead of 0.61. Lens has more information than any current standard camera sensor but potentially could be fully used in the near future.

Totally agree with you 😆 space-bandwidth product > etendue (!)

For 420 - 850 nm light the SBP is 175 - 825 megapixels. Around 500 megapixels at 500 nm.

We are excited to share our development of a new 10X lens with NA=0.50 over a 7.2 mm FOV and a 35 mm working distance (water), fully corrected from 400-850 nm. This lens will power our upcoming ExA-SPIM "2" and is available for dissemination - please get in touch if interested!

Looking forward to the @mesospim.bsky.social #lightsheet #Microscopy anniversary symposium in October with @preibischs.bsky.social @adamkglaser.bsky.social @christellelangevin.bsky.social & many more! And to the next mesoSPIM decade! 🔬 Thanks @nvladimus.bsky.social & Co for organizing it!

To look into: a Python alternative to ZEMAX... github.com/HarrisonKram...

New review out on imaging 3D cell cultures. Congratulations to Huai-Ching and @jonliu123 on leading this excellent new review! www.nature.com/articles/s41...

Curved light-sheet microscopy
www.nature.com/articles/s41...

Thermal Lensing Effects in Light-Sheet Microscopy
arxiv.org/abs/2504.02969

Sound-sheet microscopy - in this study, we introduce nonlinear sound-sheet microscopy (NSSM), a method to image targeted biological functions across cubic centimeters of opaque living tissue.

www.science.org/doi/10.1126/...

New SPIM system underway!

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue

www.biorxiv.org/content/10.1...

It’s across the board. All grants from all agencies, future ones on pause and current ones also on pause :/

Can maybe estimate based on the 35 mm full frame sensor size (36x24 mm). So probably around 1.46 micron pixels? The max rate is 3280 megapixels/sec 🤯

New 410 MP camera sensor coming in 2025 from Canon global.canon/en/news/2025...