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voigtvision.bsky.social @voigtvision.bsky.social · 9d

Philipp Keller, Benquan Wang, and Dan Flickinger at Janelia have designed a mirror objective for expansion samples that has NA 1 over a 12 mm FOV - a superimpressive setup! The only thing that's out about it is a patent application: www.freepatentsonline.com/y2023/000398...

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voigtvision.bsky.social @voigtvision.bsky.social · 9d

Thomas Naert recently published a paper on a toolbox for DNA editing with some Schmidt objective data: www.nature.com/articles/s41...

Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates - Nature Biotechnology

Genomic integration of DNA templates is made more precise through microhomology-focused design.

www.nature.com

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voigtvision.bsky.social @voigtvision.bsky.social · Aug 22

One of the people I'm indebted the most for creating stunningly beautiful #mesoSPIM samples was Martina Schaettin - who tragically passed away in March this year. To honor her memory, Elkhan Yusifov created an award - submit your best #microscopy images & videos! www.linkedin.com/company/mart...

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voigtvision.bsky.social @voigtvision.bsky.social · Aug 22

Thanks to @nvladimus.bsky.social the @mesospim.bsky.social #lightsheet #Microscope community is now on forum.image.sc/tag/mesospim !

mesoSPIM: Clear Images. Big samples. Open source. @mesospim.bsky.social · Aug 22

📣We have opened our official User Forum: forum.image.sc/tag/mesospim
In this forum you can connect with 🔬developers and the image analysis experts. Ask your burning questions about the #mesoSPIM hardware, software, and image processing, or help the beginners to get started!
Tip: use project tags!

Topics tagged mesospim

forum.image.sc

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voigtvision.bsky.social @voigtvision.bsky.social · Aug 21

Definitely the most unexpected @mesospim.bsky.social #lightsheet #microscopy application of the past years: Hunting dark matter with a lightsheet microscope! #astronomy 🔭🔬https://arxiv.org/abs/2503.20732

mesoSPIM: Clear Images. Big samples. Open source. @mesospim.bsky.social · Aug 18

💥How can light-sheet help reveal dark matter?💥
Join Prof. Laura Baudis @lbaudis.bsky.social at #mesoSPIM Symposium, Oct 13–15 in Zurich or online, to see how light-sheet fluorescence microscopy 🔬 is used to image nuclear ☢️ recoil tracks to detect elusive particles!
Register now: t.uzh.ch/1Ss

mage presenting one of the speakers for the 10 Years of mesoSPIM symposium. Background of the image is a microscopy picture showing blood vessels in magenta and glial cells in cyan on a black field. On the left, the photo of the speaker is contained in a white-rimmed circle, on the right text reads: "Laura Baudis, University of Zurich, Switzerland" followed by the talk title "Probing dark matter and neutrinos with light sheet microscopy". On the bottom right reads "10 Years of mesoSPIM Symposium”

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voigtvision.bsky.social @voigtvision.bsky.social · Aug 13

#Deeplearning-based #CRISPR DNA integration and editing by Thomas Naert & Sören Lienkamp & Co with lots of @mesospim.bsky.social #lightsheet #Microscopy data of tadpoles (and some taken with the #Schmidt #multiimmersion objective prototype!) 🔬🐸 www.nature.com/articles/s41...

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voigtvision.bsky.social @voigtvision.bsky.social · Jul 14

The @thorlabs.bsky.social LCPA2 lines are a little more straight, but a little wider as well...

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voigtvision.bsky.social @voigtvision.bsky.social · Jul 14

⚠️ #alignment #warning: When aligning with the @thorlabs.bsky.social LCPA1 & LCPA2 "T-Shirts" for 60mm cage systems: Be aware that they can have quite incorrect hole & line positioning for fine alignment work! #lightsheet #Microscopy (black lines/numbers are from an reticle in the stereo microscope)

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Reposted

Le Neuro (Institut-Hôpital neurologique de Montréal) @theneuro.bsky.social · Jul 8

Are you leading an #OpenScience initiative advancing the field of #neuroscience? Don’t miss this opportunity to be recognized for your contributions. Submit your application for the Neuro-Irv and Helga Cooper Foundation Open Science Prizes today!
👉Apply now! -link in comments
#neurology #datasharing

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voigtvision.bsky.social @voigtvision.bsky.social · Jul 2

Looks like Paula bringing macroscopic amounts of microscopic joy 🤩!

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voigtvision.bsky.social @voigtvision.bsky.social · Jun 30

Out of curiosity: What's encoded by the color code?

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voigtvision.bsky.social @voigtvision.bsky.social · Jun 4

Cool new @mesospim.bsky.social chamber design!

Jason Kirk 🔬📷 @jasonmileskirk.bsky.social · Jun 3

New chamber mount design for our @mesospim.bsky.social - magnetic kinematic bases from @thorlabs.bsky.social for the win! 3D printed (for now) bases hold the chamber snugly for precise positioning between experiments. Fill port on the front allows for media addition without disturbing imaging.

Stainless Imaging chamber for mesoSPIM with 3D printed kinematic mounts

voigtvision.bsky.social @voigtvision.bsky.social · May 27

The culmination of years of hard work by Anna Maria Reuss & Co: aDISCO - a protocol for clearing & antibody-staining human formalin-fixed paraffin-embedded (FFPE) tissue! With lots of @mesospim.bsky.social #lightsheet #Microscopy @ptrrupprecht.bsky.social & many more!
www.biorxiv.org/content/10.1...

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voigtvision.bsky.social @voigtvision.bsky.social · May 16

This is so sad to see 😢

Ryan Boileau @bffswithbiology.bsky.social · May 16

Pausing all

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voigtvision.bsky.social @voigtvision.bsky.social · May 16

Looking forward to the @mesospim.bsky.social #lightsheet #Microscopy anniversary symposium in October with @preibischs.bsky.social @adamkglaser.bsky.social @christellelangevin.bsky.social & many more! And to the next mesoSPIM decade! 🔬 Thanks @nvladimus.bsky.social & Co for organizing it!

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voigtvision.bsky.social @voigtvision.bsky.social · May 13

For scientists interested in exploring job opportunities in Europe and especially France: @cnrs.fr started an initiative "Choose CNRS": www.cnrs.fr/en/update/ch...

'Choose CNRS' – the opportunities for scientists from other countries

Four schemes are available to encourage talented young researchers and internationally-renowned scientists to consider moving to France and

www.cnrs.fr

voigtvision.bsky.social @voigtvision.bsky.social · May 8

Thanks to expansion microscopy, clever labeling, and modern segmentation approaches, doing connectomics with #light #microscopy has become feasible - huge congratulations Mojtaba & the Danzl lab at @istaresearch.bsky.social !
www.nature.com/articles/s41...

Light-microscopy-based connectomic reconstruction of mammalian brain tissue - Nature

A technique called LICONN (light-microscopy-based connectomics) allows mapping of brain tissue at synapse level and simultaneous measurement of molecular information, thus enabling quantification of c...

www.nature.com

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voigtvision.bsky.social @voigtvision.bsky.social · Apr 17

Sending lots of energy & all the best for the submissions! 💪🦾💪

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voigtvision.bsky.social @voigtvision.bsky.social · Mar 11

If you happen to need a #lightsheet #Microscope for cleared tissue on a budget: There is a Lavision Ultramicroscope on Ebay right now for $6.5k: www.ebay.com/itm/38806209... It's missing the laser unit and the PC but could serve as an excellent source of parts to build a #mesoSPIM 😉: mesospim.org

Olympus MVX10 Stereo Zoom Microscope MVX 10 & Parts BioTec Andor Neo sCMOS | eBay

Was able to do and have not received one complaint.

www.ebay.com

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voigtvision.bsky.social @voigtvision.bsky.social · Feb 13

Amazing paper! Congrats @daetwylerstephan.bsky.social @retof.bsky.social & Co 🥳🎉🎉🎉!

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voigtvision.bsky.social @voigtvision.bsky.social · Feb 12

It was great to see you again @edwardlachica3.bsky.social & sorry for the punch!

Special Ed @edwardlachica3.bsky.social · Feb 12

I love attending workshops. I see old friends. I make new friends. And then there’s stuff that punches my face. @voigtvision.bsky.social told me about his scallop objective last year but I missed this slide. NA of 1.08 in DBE with a full 11mm of working distance. Wow!

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voigtvision.bsky.social @voigtvision.bsky.social · Jan 31

There is some cool new #photophysics in town! Check out tripletimaging.co, the startup created by wongcamposd.bsky.social Dalia Ornelas-Huerta & Mackenzie Dion to explore #multiphoton #Microscopy via long-lived intermediate states:: www.tripletimaging.co/s/dyes and www.tripletimaging.co/s/proteins

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Reposted

David Schoppik @schoppik.com · Jan 30

Some really beautiful developmental biology looking at how to build an arthropod body. Legs for days! www.biorxiv.org/content/10.1...

Wild-type morphology of the Parhyale thorax and abdomen. T1-8, thoracic body segments 1-8 with corresponding legs. A1-6, abdominal body segments 1-6 with corresponding legs. A. Whole hatchling.

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voigtvision.bsky.social @voigtvision.bsky.social · Jan 27

In 2019, the #mesoSPIM and #ctASLM #lightsheet #Microscopy papers appeared back-to-back in @naturemethods.bsky.social - now Jinlong Lin, @zacsimile.bsky.social & @kevin-dean.bsky.social & Co introduce the MCT-ASLM - a chimera of both for smart multiscale #imaging!
www.biorxiv.org/content/10.1...

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voigtvision.bsky.social @voigtvision.bsky.social · Jan 27

Seems like there is a new ultracompact #femtosecond #laser source for #multiphoton #microscopy in town! 50 MHz rep rate means a 2-3 m cavity if there is a single pulse circulating in the cavity - folding that into that compact of a form factor is quite impressive!
www.laseratwork.com/products-ove...

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