Alison Chaves
@alisonchaves.bsky.social
170 followers
220 following
240 posts
Doing proteomics until I still can.
https://github.com/41ison
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Alison Chaves
@alisonchaves.bsky.social
· Aug 15
PSManalyst: A Dashboard for Visual Quality Control of FragPipe Results
FragPipe is recognized as one of the fastest computational platforms in proteomics, making it a practical solution for the rapid quality control of high-throughput sample analyses. Starting with version 23.0, FragPipe introduced the “Generate Summary Report” feature, offering .pdf reports with essential quality control metrics to address the challenge of intuitively assessing large-scale proteomics data. While traditional spreadsheet formats (e.g., tsv files) are accessible, the complexity of the data often limits user-friendly interpretation. To further enhance accessibility, PSManalyst, a Shiny-based R application, was developed to process FragPipe output files (psm.tsv, protein.tsv, and combined_protein.tsv) and provide interactive, code-free data visualization. Users can filter peptide-spectrum matches (PSMs) by quality scores, visualize protease cleavage fingerprints as heatmaps and SeqLogos, and access a range of quality control metrics and representations such as peptide length distributions, ion densities, mass errors, and wordclouds for overrepresented peptides. The tool facilitates seamless switching between PSM and protein data visualization, offering insights into protein abundance discrepancies, samplewise similarity metrics, protein coverage, and contaminants evaluation. PSManalyst leverages several R libraries (lsa, vegan, ggfortify, ggseqlogo, wordcloud2, tidyverse, ggpointdensity, and plotly) and runs on Windows, MacOS, and Linux, requiring only a local R setup and an IDE. The app is available at (https://github.com/41ison/PSManalyst.
pubs.acs.org
Alison Chaves
@alisonchaves.bsky.social
· Aug 31
Alison Chaves
@alisonchaves.bsky.social
· Aug 31
Fungal Biodiversity From the Atlantic Forest With Bioactive Metabolites Against Cutaneous Leishmaniasis
Biodiversity offers a rich source of bioactive compounds for drug discovery. We isolated Aspergillus fumigatus from compost in the Atlantic Forest and found its extract active against Leishmania (Lei...
onlinelibrary.wiley.com
Alison Chaves
@alisonchaves.bsky.social
· Aug 29
Alison Chaves
@alisonchaves.bsky.social
· Aug 28
Alison Chaves
@alisonchaves.bsky.social
· Aug 23