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Reposted by Analytical and Quantitative Light Microscopy Course

Rogério Gomes @roggomes.bsky.social · May 13

AQLM was an intense 9-day dive into advanced microscopy—blending core concepts, cutting-edge methods, and inspiring MBL history. I gained valuable skills and connections to better support LSU researchers and strengthen our imaging community. #AQLM2025 #MBL #LightMicroscopy #LSU #SIF #Microscopy 🔬🦑

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 9

And just like that, #AQLM2025 is over. Hopefully we’ll all find ourselves in the same windowless microscope room sometime soon, bead slide in hand.

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 9

This year’s esteemed Inoue Lecture was given by French optical physicist Dr. Sophie Brasselet of The Fresnel Institute. Dr. Brasselet spoke of her team’s efforts towards applying 6 degrees of PSF engineering to super resolution polarization microscopy. #AQLM2025

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 9

As a final exercise everyone got to build open source microscopes using kits available from UC2 @openuc2.bsky.social #AQLM2025

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 8

The machines learned this morning. Dr. Daniel Cortes led a lab on machine learning for image processing.

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Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · May 8

Closing @aqlm.bsky.social like it started, building a #microscope, with @openuc2.bsky.social

#microscopy #aqlm2025 @mblscience.bsky.social

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openUC2 @openuc2.bsky.social · May 7

Beautiful plant! Captured with our #lightsheet microsocpe at the #AQLM2025 @mblscience.bsky.social! Curious? openuc2.com/light-sheet-3/

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 7

Our last imaging lab was this morning (!) On to analysis and then we build more scopes and celebrate the end 🥲

Wendy Salmon 🐟🔬🧫 @fishcscells.bsky.social · May 7

Day 8 midway. It goes so fast.
#AQLM2025 @aqlm.bsky.social

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Reposted by Analytical and Quantitative Light Microscopy Course

Okolab @okolab.bsky.social · May 7

🔬 Glad to support #AQLM2025 at the #MBL.

On our partners’ workstations:
✅ UNO+ – user-friendly & high value
✅ BL3 – with Full Sample Feedback for real-time control of Temp, Humidity & CO₂ at the sample level.

👉 Already using our latest tech? Tell us what u think!

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Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · May 7

Shinya Inoue’s video #microscope at @mblscience.bsky.social

#microscopy #aqlm2025 #bioimaging

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Reposted by Analytical and Quantitative Light Microscopy Course

Wendy Salmon 🐟🔬🧫 @fishcscells.bsky.social · May 7

The AQLM Queens of Super-res lead evening discussion. Dr. Gustavsson and Dr. Lidke help us figure out how to look REALLY close. #AQLM2025

@gustavssonlab.bsky.social
@aqlm.bsky.social

Two women sit in large wooden arm chairs worn leather cushions in a high ceiling room. To the right a projector screen shows multicolor images of cells.

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 6

Arabidopsis labeled with EB1b-mNeon in green (microtubule plus end) and tubulin in magenta (microtubules). Timelapse image on the Leica Stellaris 8 laser scanning confocal. @leicamicrosystems.bsky.social @mblscience.bsky.social #AQLM2025

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 6

Ooof, those PSFs are looking *a little* asymmetric...sample prep is key here! (AKA keep Cheeto dust out of your bead sample.)
#AQLM2025

Nat Prunet @nat-prunet.bsky.social · May 6

Just a bunch of demonic point-spread functions 👹😈👺🔬

#aqlm2025 #microscopy #confocal #microscope #fluorescence

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Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · May 6

Tracking #microtubule tips in plant cells on a @zeiss-microscopy.bsky.social LSM880 #confocal #microscope 🌱🔬

#aqlm2025 #microscopymonday #microscopy #plantscience #botany #cytoskeleton #cellbio

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 6

Dr. Palmer gives an amazing lecture on dyes, fluorescent proteins, and FRET/FLIM. A chemist who makes non-chemists understand the chemistry! #AQLM2025

AmyPalmerZinc @amypalmerzinc.bsky.social · May 6

Lots of fun and great conversation about fluorescent proteins, probes, biosensors and Fret at @aqlm.bsky.social course at MBL. Also great to be in such close proximity to Shimomura GFP!

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 5

Tracking Arabidopsis microtubule tips on the NSPARC 🌈

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 5

Arabidopsis with EB1b-mNEON labeling the plus end of microtubules 🌱 Imaged on the Nikon AX-R laser scanning confocal with NSPARC. #AQLM2025

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 5

Live cell lab: understanding the trade offs between acquisition time, SNR, spatial resolution, and cell survival on different microscope systems. You can’t have it all, but ideally you can have enough for meaningful analysis. #AQLM2025

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Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · May 4

The back focal plane! Today Dr. Chris Bjornsson demonstrated Abbe’s theory of resolution by manipulating the light passing through the back focal plane. Here he is using a lens to focus the back focal plane onto the camera for us to see. #AQLM2025