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BioVoxxel (Jan Brocher) @biovoxxel.de · 21h

🔬 Blinded by colors?

Color ≠ Intensity!

Quantifying intensities from RGB images (e.g. fluorescence, blots, ...) is mostly misleading!

Check your image data type carefully!

Learn more in Newsletter No. 5 - To analyze RGB images, or NOT to analyze RGB images?

Read and subscribe: bit.ly/46mYBL7

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BioVoxxel (Jan Brocher) @biovoxxel.de · 13d

Really nice. Looks cool and all without fancy equipment 👏🎬👌

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BioVoxxel (Jan Brocher) @biovoxxel.de · 14d

So, now the article also made it into the newsletter and is available!

BioVoxxel (Jan Brocher) @biovoxxel.de · 14d

Bio-Imaging with Shakespeare: pseudocoloring (LUTs) — reveal hidden imaging issues and highlight important data.

✔ Spot noise, shading & saturation
✔ Map data for spatial insight
✔ Make color accessible to all
✔ Why to use a calibration bar

Full guide, tools & tutorial inside 👇
buff.ly/8x7Shiq

BioVoxxel (Jan Brocher) @biovoxxel.de · 14d

Bio-Imaging with Shakespeare: pseudocoloring (LUTs) — reveal hidden imaging issues and highlight important data.

✔ Spot noise, shading & saturation
✔ Map data for spatial insight
✔ Make color accessible to all
✔ Why to use a calibration bar

Full guide, tools & tutorial inside 👇
buff.ly/8x7Shiq

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BioVoxxel (Jan Brocher) @biovoxxel.de · 26d

To this end, the biweekly "Bio-Imaging with Shakespeare" LinkedIn Newsletter might be of interest.
It discusses different aspects of image processing, analysis and visualization techniques and outlines solutions for some issues mentioned in the article from @jencwaters.bsky.social.

bit.ly/46mYBL7

Bio-Imaging with Shakespeare | LinkedIn

Biweekly takes on bio-image analysis, processing techniques, insight into tools and trade‑offs as well as best practices

bit.ly

Reposted by BioVoxxel (Jan Brocher)

Rylie Walsh @ryliewalsh.bsky.social · 27d

Everyone who does quantitative microscopy should read this! It’s amazing how many ways we can bias our imaging experiments without realizing we’re doing it.

Journal of Cell Biology @jcb.org · 27d

In 2019, Anna Jost & @jencwaters.bsky.social reviewed best practices for validating quantitative #microscopy methods & discuss strategies to avoid unconscious bias in imaging experiments rupress.org/jcb/article/...

📕 In #Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...

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BioVoxxel (Jan Brocher) @biovoxxel.de · 28d

No. 3 - To Script it, or not to Script it?

ImageJ's GenericDialog or ImageJ2 Script Parameter driven dialogs.
Which one's for you and fits your needs?

BioVoxxel’s latest "Bio-Imaging with Shakespeare" Newsletter explores the trade-offs:
buff.ly/8x7Shiq

#BioImageAnalysis #ImageJ #SciJava #IJMacro

BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 30

Amazing BlueSky Summer Sale

Last Chance — ONLY 1 DAY LEFT

For any of the #BioVoxxel online self-learning courses

buff.ly/o64cW9Y

Use the code "BSKY101010" at checkout to redeem your discount.

Hurry, and see if any codes are left for you!

Only 10 codes until August 31st 2025 available!🏃‍♂️

BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 27

In the 2nd edition of Bio-Imaging with Shakespeare we meet a pesky gremlin — uneven illumination (aka shading, intensity non-uniformity). 🎭🔬 💻

It can quietly bias your segmentation and image analysis results!

So, detect it early, correct it appropriately!

Subscribe on LinkedIn buff.ly/cLPh8eZ

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BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 21

Amazing BlueSky Summer Sale

Your choice: Any of the #BioVoxxel online self-learning courses

buff.ly/o64cW9Y

The first 10 participants that enroll in the next 10 days get 10% OFF

Use the code "BSKY101010" at checkout to redeem your discount.

Hurry, only 10 chances until August 31st 2025!🏃‍♂️🏃‍♀️

BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 20

BlueSky Summer Sale starting tomorrow 8:00 AM (CET) for all individual online courses.

Make sure you are ready for the run: 10% Off for the first 10 that enroll in the next 10 days.

Check out the courses, so you are ready to choose faster then your competitors 🏃‍♀️ 🏅

buff.ly/o64cW9Y

BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 20

Embark on your journey to become an #ImageAnalysis champion today! 🏅 🏆

Sign up here: buff.ly/o64cW9Y

1. Basic Microscopic Image Analysis
2. Automate Analyses with #ImageJ #Macro #Scripting
3. Transform Pixels to Patterns with #MachineLearning

#Fiji #BioVoxxel

Any Questions? -> DM me Bsky

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BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 13

I am stunned!
First edition of the "Bio-Imaging with Shakespeare" LinkedIn Newsletter (buff.ly/8x7Shiq) is out for 15h and already 130 subscribers.

If you are interested in the topic FOLLOW ME on LinkedIn and Bsky and SUBSCRIBE
on LinkedIn buff.ly/cLPh8eZ

#bioimageanalysis #biovoxxel #fiji

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BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 13

Eager to stir curiosity and foster dialogue on varied viewpoints? Connect with me on LinkedIn, subscribe to the newsletter (Follow here: buff.ly/cLPh8eZ), and spread the word within your network. Let's engage and exchange ideas!

#BioImaging #ImageAnalysis #BioVoxxel #Conversation

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BioVoxxel (Jan Brocher) @biovoxxel.de · Aug 13

To interpolate or not to interpolate ? That is the question!

1st edition of the Bio-Imaging with Shakespeare Newsletter I start a biweekly series containing takes on bio-image analysis, processing techniques, insight into tools and trade‑offs as well as best practices.

Subscribe buff.ly/cLPh8eZ

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BioVoxxel (Jan Brocher) @biovoxxel.de · Jul 27

🧗 Free Climber: From Pixels to Patterns: Applied Machine Learning Tools for Advanced Image Analysis

Reach the summit with ease and savely by applying state of the art #machinelearning and #deeplearning tool (almost) without programming (all GUI based) and connect them in #Fiji

youtu.be/lee4OiV5E6g

From Pixels to Patterns: Applying Machine Learning Tools for Advanced Image Analysis

YouTube video by Jan Brocher

youtu.be

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BioVoxxel (Jan Brocher) @biovoxxel.de · Jul 27

🏔 Climber: Image Analysis Automation with ImageJ Macro Scripting

Take image analysis automation to the next level. Without any prior programming knowledge, this course will take you the fear if hights and complexity of scripting step-by-step

youtu.be/OaXdxMdaFUE

#ImageJ #Fiji #Macros #Programming

ImageJ Macro Scripting for Automatic Image Analysis Online Course

YouTube video by Jan Brocher

youtu.be

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BioVoxxel (Jan Brocher) @biovoxxel.de · Jul 27

🥾 Hiker: Basic Microscopic Image Processing and Analysis

Learn all the basics of #imagesegmentation to manage flexible #automation of area and object extraction including #qualitycontrol for less bias and reduced errors.

youtu.be/s2T3B5Q8A7c

#ImageJ #Fiji #bioimageanalysis #microscopy

Online Course: Basic Microscopic Image Processing and Analysis Online Course

YouTube video by Jan Brocher

youtu.be

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BioVoxxel (Jan Brocher) @biovoxxel.de · Jul 27

In #imageanalysis like in hiking, preparation and training is key to master challenges ahead.

🥾 Hiker: Basic Microscopic Image Processing and Analysis
🏔 Climber: ImageJ Macro Scripting
🧗 Free Climber: Applied #machinelearning

Choose your challenge 👉 www.biovoxxel.de/individual-w...

#Fiji #ImageJ