In addition to laying the groundwork for future engineering,
@rfw.bsky.social and I hope our characterization of several evolutionarily-accessible, activating mutations will provide insights into TnpB evolution and TnpB’s function within insertion sequences. Congrats to the entire amazing team!

Excited to share our new preprint on mutational scanning of the TnpB RNP! We identify several highly activating, single-position mutations in TnpB’s protein and reRNA. These datasets enabled us to engineer enhanced TnpB protein variants (>50x plant editing levels compared to WT).

now i am!