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Brochado Lab

@brochadolab.bsky.social

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Microbe lover, Assistant professor @Uni_Tue @CoE_CMFI working on system biology of bacteria & antibiotics. @EMBL alumni, @Uni_WUE alumni www.brochadolab.com

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Reposted by Brochado Lab

PLOS Biology @plosbiology.org · Jul 23

How are bacterial transport machineries regulated by external cues? @brochadolab.bsky.social &co identify environmental chemicals that regulate #transporter transcription, identifying the TF Rob as a key modulator of #EffluxPump expression in #Ecoli #AMR @plosbiology.org 🧪 plos.io/46pUM9Q

Left: Compound-promoter interaction network. Fifty-three significant compound-promoter interactions are shown as edges in a Sankey diagram connecting the compounds (left, source nodes) to the promoters (right, target nodes). Edge thickness represents mean Z-scores (n = 8), while node size represents the total number of interactions. Right: Proposed model for the molecular mechanism of caffeine-ciprofloxacin antagonism. Caffeine triggers expression of MicF small RNA in a Rob-dependent manner, which then binds to the 5′-UTR of ompF mRNA to inhibit and decrease OmpF protein levels. This ultimately prevents ciprofloxacin from entering the cell, resulting in caffeine-ciprofloxacin antagonism

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Brochado Lab @brochadolab.bsky.social · Jul 23

Finally out! A small screen helps understand a little more about how E. coli regulates major transporters, and reveals how caffeine induces phenotypical antibiotic resistance. Huge thanks to collab @andrenmateus.bsky.social (Umeå) and Christian L. Müller (Munich)!
journals.plos.org/plosbiology/...

Systematic screen uncovers regulator contributions to chemical cues in Escherichia coli

Transport machineries are crucial for antibiotic resistance, but their regulation by external cues is poorly understood. This study identifies environmental chemicals that regulate transporter transcr...

journals.plos.org

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Reposted by Brochado Lab

EMBL @embl.org · Feb 27

EMBL will coordinate the MSCA Doctoral Network – Exploring Novel Drivers of Anti-Microbial Resistance (ENDAMR). 🦠

The network will fund 10 PhD positions, providing training to better equip researchers in Europe to develop new strategies for tackling antimicrobial resistance.

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Reposted by Brochado Lab

EMBL @embl.org · Feb 27

The ENDAMR Supervisory Board held its kick-off meeting at EMBL Heidelberg, where they reviewed scientific and strategic plans.

👉 Stay up-to-date on ENDAMR network-wide opportunities here: endamr.eu

#MSCA #DoctoralNetwork #AntimicrobialResistance #ENDAMR #ResearchTraining

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Brochado Lab @brochadolab.bsky.social · Feb 20

Brochado and Maier labs in Germany are looking for a PhD student to study horizontal gene transfer in bacteria. ENDAMR Marie Curie doctoral network (endamr.eu) - an amazing opportunity to work with top scientists in the field all around Europe!
www.cmfi.uni-tuebingen.de/mariecuriephd

endamr.eu

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Reposted by Brochado Lab

Susanne Brenzinger @subrenz.bsky.social · Jan 3

Can a phage defence system interfere with antibiotic action? 💊 Yes, it can! CBASS is triggered by antifolates and turns these mostly bacteriostatic drugs into bactericidal ones in bugs like V. cholerae. 🦠 www.nature.com/articles/s41564-023-01556-y Read full here: rdcu.be/du20r

The Vibrio cholerae CBASS phage defence system modulates resistance and killing by antifolate antibi...

High-throughput growth assays show that cyclic-oligonucleotide-based anti-phage signalling systems modulate Vibrio cholerae sensitivity to antifolates, and ultimately trigger cell lysis by these drugs...

www.nature.com

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