Light-inducible control of protein subcellular localization holds great promise for synthetic biology applications and insights into basic cell biology. Here the authors develop a genetically-encoded light-inducible nuclear export system and apply it to a synthetic repressor and p53 transcriptional activity.

Université de Montpellier, CNRS, UMR9002. This protocol describes how to efficiently set up and perform automated analyses of live cell imaging data obtained from tracking the nucleocytoplasmic trans...

McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.

Genome function requires regulated genome motion. However, tools to directly observe this motion in vivo have been limited in coverage and resolution. Here we introduce an approach to tile mammalian c...

The Max Planck Institute for Molecular Biomedicine in Münster, Germany, has an opening for a