I see this with the VPN at work as well: Cloudflare tends to throw captchas much more frequently. It is what it is.

yeah, that’s weird. But if something goes wrong, you can try the one I’m using.

Speedwise, Proton VPN is fine for that. Just constant checks of my humanity.

I am slowly wondering if they might be on to something there 😉

Not reassuring this early separation already in 2 days

Fits perfectly with this not being a study on the effects of drinking coffee but of sudden withdrawal of coffee. The fact that this was published without the actual control group (people who don't drink coffee at all) is quite schocking. Doesn't speak for the journal.

Sorry @erictopol.bsky.social it doesn't. All participants were normal coffee drinkers. Therefore, the coffee-drinker arm is the Control arm. The intervention of going cold turkey increased the hazard of AF by 64% (100/0.61). The whole report is written from the wrong perspective.

Purified proteins? In cultured cells? In an intact organism?

On cells, I faintly recall the mitochondrial outer membrane assay for cultured cells. Just to add more options to this thread 😁

Isn't the biggest issue with BiFC that the probes themselves have a binding affinity that is non-zero?

I would lean towards some form of FRET assay. Ideally using fluorescence lifetime as readout. But I might be a bit biased 😉

You haven't by chance started using a VPN?

This is the price for the last DC SfN having sunshine and temps in the sixties 🫣

Don't let them check the weather report until the plane doors are closed ;-)

Whenever I needed a strongly fluorescent sample that had some structure and depth, I simply grabbed a fresh leaf outside. This was multiphoton, but there should be strong autofluorescence in 1P as well.