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dfachinetti.bsky.social @dfachinetti.bsky.social · 2d

Thanks to @erhardtlab.bsky.social for the news and views on our paper on DNA methylation¢romere www.nature.com/articles/s41....

Dipping into the function of DNA methylation at centromeres - Nature Genetics

Human centromeres contain a small chromatin region with low levels of DNA cytosine methylation that resides with CENP-A. Salinas-Luypaert et al. find a role of DNA methylation in maintaining the size and function of centromeres by controlling the binding affinity of key centromere components.

www.nature.com

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dfachinetti.bsky.social @dfachinetti.bsky.social · 4d

10-year celebration of the Fachinetti team!
Thank you to all my amazing current and former members for making these two days so special for me! I feel so lucky to have met you all!

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Reposted

CNRS Biologie @cnrsbiologie.bsky.social · 29d

#ResultatScientifique🔎| La méthylation de l’ADN agit comme un code caché des centromères, garantissant une division cellulaire stable et freinant l’instabilité génomique 🧬
🤝@cnrs-paris.bsky.social @institutcurie.bsky.social
✍️ @dfachinetti.bsky.social
📕 @natgenet.nature.com |https://buff.ly/hxb1yxh

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Reposted

Nature Genetics @natgenet.nature.com · Sep 5

📢ONLINE @natgenet.nature.com

📰DNA methylation influences human centromere positioning and function.

By Catalina Salinas-Luypaert, @dfachinetti.bsky.social, @naltemose.bsky.social and colleagues.

⬇️

www.nature.com/articles/s41...

DNA methylation influences human centromere positioning and function - Nature Genetics

Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

www.nature.com

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Reposted

Institut Curie @institutcurie.bsky.social · Sep 4

▶️ Pour en savoir plus
⏩ Lire l'actu : curie.fr/actualite/di...
⏩ Lire la publication : www.nature.com/articles/s41...

DNA methylation influences human centromere positioning and function - Nature Genetics

Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

www.nature.com

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

Finally we want to thank our amazing collaborators @foijer-lab.bsky.social , Geert Kops and team, Claire Francastel and team and @khmiga.bsky.social and team.

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

#6 In summary, this work carried out at @institutcurie.bsky.social @cnrs.fr @stanforduniversity.bsky.social and supported by @agencerecherche.bsky.social @fondationarc.bsky.social @chanzuckerberg.bsky.social ‪provides the first evidence that DNA methylation directly influences centromere function

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

#5 We also found that gradual methylation loss allows most cells to adapt and survive, whereas rapid loss causes aneuploidy and cell death. This slow adaptation mirrors what often happens during cancer progression.

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

#4 We show that loss of DNAme leads to disruption of the centromere’s bipartite structure and also controls the presence of another centromere-associated protein, CENP-B. Excessive CENP-B makes centromeric DNA more fragile and prone to breakage.

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

#3 Using DiMeLo-seq, Rosa Lee and Danilo Dubocanin from the Altemose team mapped CENP-A binding with high precision. They found that when DNA methylation is lost, CENP-A spreads to inappropriate regions, causing centromere alterations and chromosomal imbalances.

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

#2 During her PhD, Catalina Salinas-Luypaert developed human cell models that allowed us to directly alter DNA methylation at centromeres and observe the consequences. We show that DNA methylation is critical to keep CENP-A correctly positioned and centromeres functioning.

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dfachinetti.bsky.social @dfachinetti.bsky.social · Sep 4

#1 Centromeres are epigenetic loci defined by CENP-A, positioned in unmethylated DNA flanked by highly methylated regions. Our work, published in @natgenet.nature.com in collaboration with @naltemose.bsky.social investigates the role of DNAme at human centromeres www.nature.com/articles/s41...

DNA methylation influences human centromere positioning and function - Nature Genetics

Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

www.nature.com

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dfachinetti.bsky.social @dfachinetti.bsky.social · Aug 15

And great time with our long-term friends, the Holland!

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dfachinetti.bsky.social @dfachinetti.bsky.social · Aug 15

Not to forget a great dinner and time with my ex neighbor labs @odlab.bsky.social . San Diego is always the best place to be ♥️

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dfachinetti.bsky.social @dfachinetti.bsky.social · Aug 15

Finally, superb time at UPENN with my friends Alessandro Gardini, Ben Black and @glennislogsdon.bsky.social and great discussion with the faculty members. I felt at home!

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dfachinetti.bsky.social @dfachinetti.bsky.social · Aug 15

Amazing visit also to @stanfordpress.bsky.social with my friend @naltemose.bsky.social and great time with @astraight.bsky.social team members. What a campus and what great labs! You guys rock!

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dfachinetti.bsky.social @dfachinetti.bsky.social · Aug 15

Great time with @hirofunabiki.bsky.social @rockefelleruniv.bsky.social with amazing discussion with @prasad.bsky.social , @johnmaciejowski.bsky.social @agnelsfeir.bsky.social and Simona Giunta. Thanks also to Hiro’s lab for all the great chat! Happy to have seen my PhD friend Fabio Vanoli

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dfachinetti.bsky.social @dfachinetti.bsky.social · Jun 3

Thank Nico. We feel the same way! More exciting research to come between our teams!

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dfachinetti.bsky.social @dfachinetti.bsky.social · Jun 2

Congrats to the team! Happy that we could help!

Nicolas Manel Lab @manellab.bsky.social · Jun 2

Thrilled to share our latest @cp-cell.bsky.social.
We present VICAR (VIral-induced Centromeric DNA Amplification and Recognition), a new defense system to detect viruses in the nucleus based on nuclear cGAS.
www.cell.com/cell/abstrac...

Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS

Herpesvirus proteins disrupt centromeres, triggering centromeric DNA amplification and local nuclear activation of the nucleic acid sensor cGAS. This reveals an immune surveillance mechanism in the nu...

www.cell.com

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dfachinetti.bsky.social @dfachinetti.bsky.social · May 21

Hi all, my team institut-curie.org/team/fachine... @institutcurie.bsky.social is looking for a Bioinformatician.
You can find more details about the position here: euraxess.ec.europa.eu/jobs/344508 Is anyone looking for a job in the lovely Paris?