epithelial mechanics fan club
@epimechfc.bsky.social
📚 We're your source for papers on various #EpithelialMechanics topics 🔍 & platform to share your research and passion 🧫🔬
Like to write a thread? Please DM us!
💬 @onenimesa.bsky.social & @juliaeckert.bsky.social
👉 https://epithelialmechanics.github.io
Like to write a thread? Please DM us!
💬 @onenimesa.bsky.social & @juliaeckert.bsky.social
👉 https://epithelialmechanics.github.io
Caldarelli, P., Chamolly, A., Villedieu, A., Alegria-Prévot, O., Phan, C., Gros, J., & Corson, F. (2024). Self-organized tissue mechanics underlie embryonic regulation. Nature, #EpithelialMechanics www.nature.com/articles/s41...
February 11, 2026 at 8:01 AM
Caldarelli, P., Chamolly, A., Villedieu, A., Alegria-Prévot, O., Phan, C., Gros, J., & Corson, F. (2024). Self-organized tissue mechanics underlie embryonic regulation. Nature, #EpithelialMechanics www.nature.com/articles/s41...
Afting, C., Walther, T., Drozdowski, O. M., Schlagheck, C., Schwarz, U. S., Wittbrodt, J., & Göpfrich, K. (2024). DNA microbeads for spatio-temporally controlled morphogen release within organoids. Nature Nanotechnology www.nature.com/articles/s41... #EpithelialMechanics
February 10, 2026 at 8:00 AM
Afting, C., Walther, T., Drozdowski, O. M., Schlagheck, C., Schwarz, U. S., Wittbrodt, J., & Göpfrich, K. (2024). DNA microbeads for spatio-temporally controlled morphogen release within organoids. Nature Nanotechnology www.nature.com/articles/s41... #EpithelialMechanics
Flipper-TR help introduce membrane mechanics to measurable cell biology experiments. Its future lies in combining composition, mechanics, and protein interactions, and interpreting it with care.
Contact me if you need help, advice, or discussion!
Thanks to @epimechfc.bsky.social for this platform💙
Contact me if you need help, advice, or discussion!
Thanks to @epimechfc.bsky.social for this platform💙
February 8, 2026 at 7:59 AM
Flipper-TR help introduce membrane mechanics to measurable cell biology experiments. Its future lies in combining composition, mechanics, and protein interactions, and interpreting it with care.
Contact me if you need help, advice, or discussion!
Thanks to @epimechfc.bsky.social for this platform💙
Contact me if you need help, advice, or discussion!
Thanks to @epimechfc.bsky.social for this platform💙
📄 MANY cool applications for the probe are on the way or have been published... as in mitochrondrial fission
🔗 bsky.app/profile/epim...
from @sulianamanley.bsky.social or to assess peptide interaction to in vitro lipid bilayers
🔗 bsky.app/profile/epim...
from @javierespadas.bsky.social
🔗 bsky.app/profile/epim...
from @sulianamanley.bsky.social or to assess peptide interaction to in vitro lipid bilayers
🔗 bsky.app/profile/epim...
from @javierespadas.bsky.social
February 8, 2026 at 7:59 AM
📄 MANY cool applications for the probe are on the way or have been published... as in mitochrondrial fission
🔗 bsky.app/profile/epim...
from @sulianamanley.bsky.social or to assess peptide interaction to in vitro lipid bilayers
🔗 bsky.app/profile/epim...
from @javierespadas.bsky.social
🔗 bsky.app/profile/epim...
from @sulianamanley.bsky.social or to assess peptide interaction to in vitro lipid bilayers
🔗 bsky.app/profile/epim...
from @javierespadas.bsky.social
📄 A major effort is carried out by the community to benchmark Flipper-TR alongside other membrane probes. This elegant paper from @sciezgin.bsky.social quantified the FLIM response of several probes in parallel
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
📄 A major effort is carried out by the community to benchmark Flipper-TR alongside other membrane probes. This elegant paper from @sciezgin.bsky.social quantified the FLIM response of several probes in parallel
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
📄 Methods: A practical FLIM analysis-focused guide: acquisition, fitting strategies, phasors, and interpretation. It clearly explains how lifetime reflects lipid packing, and where over-interpretation of “tension” can go wrong.
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
📄 Methods: A practical FLIM analysis-focused guide: acquisition, fitting strategies, phasors, and interpretation. It clearly explains how lifetime reflects lipid packing, and where over-interpretation of “tension” can go wrong.
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
📄 Methods: Experimental best practices covering labelling, imaging, and controls across systems. Essential reading to avoid technical artifacts and misleading comparisons.
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
📄 Methods: Experimental best practices covering labelling, imaging, and controls across systems. Essential reading to avoid technical artifacts and misleading comparisons.
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
📄 Tension gradients during migration. Using Flipper-TR FLIM, we showed that membrane tension gradients exist in all adherent cells and are actively maintained by actin and adhesion, not passively equilibrating. @rouxlab.bsky.social and Aumeier lab
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
📄 Tension gradients during migration. Using Flipper-TR FLIM, we showed that membrane tension gradients exist in all adherent cells and are actively maintained by actin and adhesion, not passively equilibrating. @rouxlab.bsky.social and Aumeier lab
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
📄 In cells, osmotic shocks shift Flipper-TR lifetime consistently with increased or decreased membrane tension, provided composition is controlled. This paper also compared quantitatively tether force and F-TR lifetime measurements @chloeroffay.bsky.social
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
📄 In cells, osmotic shocks shift Flipper-TR lifetime consistently with increased or decreased membrane tension, provided composition is controlled. This paper also compared quantitatively tether force and F-TR lifetime measurements @chloeroffay.bsky.social
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
💡 By tuning lipid composition and applied tension in GUVs, Flipper-TR lifetime could be quantitatively related to membrane mechanical state, establishing the physical basis for cellular measurements made later.
February 8, 2026 at 7:59 AM
💡 By tuning lipid composition and applied tension in GUVs, Flipper-TR lifetime could be quantitatively related to membrane mechanical state, establishing the physical basis for cellular measurements made later.
📄 The first study from @rouxlab.bsky.social in cells showed that planarizable Flipper probes report lipid composition, packing and phase state in GUVs, and respond to tension via changes in lipid order, both in model membranes and cells. @colomlab.bsky.social
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
📄 The first study from @rouxlab.bsky.social in cells showed that planarizable Flipper probes report lipid composition, packing and phase state in GUVs, and respond to tension via changes in lipid order, both in model membranes and cells. @colomlab.bsky.social
🔗 bsky.app/profile/epim...
🔗 bsky.app/profile/epim...
💡 Core principle:
Flipper-TR reports changes in the lipid packing / lateral pressure of the bilayer, which is shaped by the physico-chemical environment. These are many factors: composition, phase behaviour, curvature, and tension. Tension acts through lipid packing at the nanoscale.
Flipper-TR reports changes in the lipid packing / lateral pressure of the bilayer, which is shaped by the physico-chemical environment. These are many factors: composition, phase behaviour, curvature, and tension. Tension acts through lipid packing at the nanoscale.
February 8, 2026 at 7:59 AM
💡 Core principle:
Flipper-TR reports changes in the lipid packing / lateral pressure of the bilayer, which is shaped by the physico-chemical environment. These are many factors: composition, phase behaviour, curvature, and tension. Tension acts through lipid packing at the nanoscale.
Flipper-TR reports changes in the lipid packing / lateral pressure of the bilayer, which is shaped by the physico-chemical environment. These are many factors: composition, phase behaviour, curvature, and tension. Tension acts through lipid packing at the nanoscale.
The planarization changes the energies of the excited and ground states of the fluorophore. This means that the fluorescence properties of Flipper-TR depend on its conformation: fluorescence lifetime, quantum yield, and excitation wavelength.
February 8, 2026 at 7:59 AM
The planarization changes the energies of the excited and ground states of the fluorophore. This means that the fluorescence properties of Flipper-TR depend on its conformation: fluorescence lifetime, quantum yield, and excitation wavelength.
How does Flipper-TR work?
Flipper-TR inserts in the lipid bilayer. Its mechanosensitive core rotates, sensing the lipid packing. The electron-rich head group confers the plasma membrane specificity... but other versions exist for alternative targeting!
🔗 bsky.app/profile/epim...
Flipper-TR inserts in the lipid bilayer. Its mechanosensitive core rotates, sensing the lipid packing. The electron-rich head group confers the plasma membrane specificity... but other versions exist for alternative targeting!
🔗 bsky.app/profile/epim...
February 8, 2026 at 7:59 AM
How does Flipper-TR work?
Flipper-TR inserts in the lipid bilayer. Its mechanosensitive core rotates, sensing the lipid packing. The electron-rich head group confers the plasma membrane specificity... but other versions exist for alternative targeting!
🔗 bsky.app/profile/epim...
Flipper-TR inserts in the lipid bilayer. Its mechanosensitive core rotates, sensing the lipid packing. The electron-rich head group confers the plasma membrane specificity... but other versions exist for alternative targeting!
🔗 bsky.app/profile/epim...
First, why Flipper-TR? It comes from swimming fins, also called swimming flippers, because of their shape! coined by the first author of the original paper, Marta Dal Molin, at @matilegroup.bsky.social. TR stands for ‘tension reporter’.
Here’s a bit of history: www.unige.ch/campus/numer...
Here’s a bit of history: www.unige.ch/campus/numer...
February 8, 2026 at 7:59 AM
First, why Flipper-TR? It comes from swimming fins, also called swimming flippers, because of their shape! coined by the first author of the original paper, Marta Dal Molin, at @matilegroup.bsky.social. TR stands for ‘tension reporter’.
Here’s a bit of history: www.unige.ch/campus/numer...
Here’s a bit of history: www.unige.ch/campus/numer...
Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells.
Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!
Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!
February 8, 2026 at 7:59 AM
Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells.
Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!
Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!
Espadas, J., Souza, D. P., Hakala, M., García-Arcos, J. M., Tran, J., Kumar, A., Marcuello, C., Merino, A., ..., Toret, C. P., & Roux, A. (2025). Molecular basis for cellular compartmentalization by an ancient membrane fission mechanism. bioRxiv. #EpithelialMechanics
buff.ly/OQv9qMw
buff.ly/OQv9qMw
February 6, 2026 at 11:01 AM
Espadas, J., Souza, D. P., Hakala, M., García-Arcos, J. M., Tran, J., Kumar, A., Marcuello, C., Merino, A., ..., Toret, C. P., & Roux, A. (2025). Molecular basis for cellular compartmentalization by an ancient membrane fission mechanism. bioRxiv. #EpithelialMechanics
buff.ly/OQv9qMw
buff.ly/OQv9qMw
Mandal, T., Roux, A., & García-Arcos, J. M. (2025). Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM. bioRxiv. #EpithelialMechanics
buff.ly/NAytZaR
buff.ly/NAytZaR
February 6, 2026 at 8:01 AM
Mandal, T., Roux, A., & García-Arcos, J. M. (2025). Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM. bioRxiv. #EpithelialMechanics
buff.ly/NAytZaR
buff.ly/NAytZaR
Roffay, C., García-Arcos, J. M., Chapuis, P., López-Andarias, J., Schneider, F., ...., Roux, A., & Mercier, V. (2024). Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes. Nature protocols, 19(12), 3457–3469. #EpithelialMechanicsReview
buff.ly/mTlcVwn
buff.ly/mTlcVwn
February 5, 2026 at 8:00 AM
Roffay, C., García-Arcos, J. M., Chapuis, P., López-Andarias, J., Schneider, F., ...., Roux, A., & Mercier, V. (2024). Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes. Nature protocols, 19(12), 3457–3469. #EpithelialMechanicsReview
buff.ly/mTlcVwn
buff.ly/mTlcVwn
Mahecic, D., Carlini, L., Kleele, T., Colom, A., Goujon, A., Matile, S., Roux, A., & Manley, S. (2021). Mitochondrial membrane tension governs fission. Cell reports, 35(2), 108947. #EpithelialMechanics
buff.ly/BtTomnv
buff.ly/BtTomnv
February 4, 2026 at 11:01 AM
Mahecic, D., Carlini, L., Kleele, T., Colom, A., Goujon, A., Matile, S., Roux, A., & Manley, S. (2021). Mitochondrial membrane tension governs fission. Cell reports, 35(2), 108947. #EpithelialMechanics
buff.ly/BtTomnv
buff.ly/BtTomnv
García-Arcos, J. M., Mehidi, A., Sanchez-Velasquez, J., Guillamat, P., Tomba, C., Houzet, L., ..., Aumeier, C., & Roux, A. (2025). Adherent cells sustain membrane tension gradients independently of migration. Nature communications, 16(1), 10539. #EpithelialMechanics
buff.ly/bVxNtN5
buff.ly/bVxNtN5
February 4, 2026 at 8:00 AM
García-Arcos, J. M., Mehidi, A., Sanchez-Velasquez, J., Guillamat, P., Tomba, C., Houzet, L., ..., Aumeier, C., & Roux, A. (2025). Adherent cells sustain membrane tension gradients independently of migration. Nature communications, 16(1), 10539. #EpithelialMechanics
buff.ly/bVxNtN5
buff.ly/bVxNtN5
Ragaller, F., Sjule, E., Urem, Y. B., ..., Blom, H., & Sezgin, E. (2024). Quantifying Fluorescence Lifetime Responsiveness of Environment-Sensitive Probes for Membrane Fluidity Measurements. The journal of physical chemistry. B, 128(9), 2154–2167. #EpithelialMechanics
buff.ly/YjCWSfP
buff.ly/YjCWSfP
February 3, 2026 at 11:00 AM
Ragaller, F., Sjule, E., Urem, Y. B., ..., Blom, H., & Sezgin, E. (2024). Quantifying Fluorescence Lifetime Responsiveness of Environment-Sensitive Probes for Membrane Fluidity Measurements. The journal of physical chemistry. B, 128(9), 2154–2167. #EpithelialMechanics
buff.ly/YjCWSfP
buff.ly/YjCWSfP
Roffay, C., Molinard, G., ..., & Roux, A. (2021). Passive coupling of membrane tension and cell volume during active response of cells to osmosis. Proceedings of the National Academy of Sciences of the United States of America, 118(47), e2103228118. #EpithelialMechanics
buff.ly/r1Hj7F6
buff.ly/r1Hj7F6
February 3, 2026 at 8:01 AM
Roffay, C., Molinard, G., ..., & Roux, A. (2021). Passive coupling of membrane tension and cell volume during active response of cells to osmosis. Proceedings of the National Academy of Sciences of the United States of America, 118(47), e2103228118. #EpithelialMechanics
buff.ly/r1Hj7F6
buff.ly/r1Hj7F6
Straková, K., López-Andarias, J., Jiménez-Rojo, N., ..., Sakai, N., & Matile, S. (2020). HaloFlippers: A General Tool for the Fluorescence Imaging of Precisely Localized Membrane Tension Changes in Living Cells. ACS central science, 6(8), 1376–1385. #EpithelialMechanics
buff.ly/sJvPI4R
buff.ly/sJvPI4R
February 2, 2026 at 11:01 AM
Straková, K., López-Andarias, J., Jiménez-Rojo, N., ..., Sakai, N., & Matile, S. (2020). HaloFlippers: A General Tool for the Fluorescence Imaging of Precisely Localized Membrane Tension Changes in Living Cells. ACS central science, 6(8), 1376–1385. #EpithelialMechanics
buff.ly/sJvPI4R
buff.ly/sJvPI4R
Licari, G., Strakova, K., Matile, S., & Tajkhorshid, E. (2020). Twisting and tilting of a mechanosensitive molecular probe detects order in membranes. Chemical science, 11(22), 5637–5649. #EpithelialMechanics
buff.ly/JiKLFzY
buff.ly/JiKLFzY
February 2, 2026 at 8:01 AM
Licari, G., Strakova, K., Matile, S., & Tajkhorshid, E. (2020). Twisting and tilting of a mechanosensitive molecular probe detects order in membranes. Chemical science, 11(22), 5637–5649. #EpithelialMechanics
buff.ly/JiKLFzY
buff.ly/JiKLFzY