@femijohn.bsky.social
Reposted
Our work expanding the Large Serine Integrase toolkit is finally out! We used alphafold-multimer to identify likely recombination directionality factors and tested them experimentally. Much credit to fantastic postdoc Heewhan Shin.
@heewhanshin.bsky.social
#Alphafold
#MobileGeneticElements
@heewhanshin.bsky.social
#Alphafold
#MobileGeneticElements
⭐NAR Breakthrough!⭐
AlphaFold2 predicted LSI–RDF interactions and experimentally validated several new functional pairs.
@phoeberice.bsky.social @femijohn.bsky.social
doi.org/10.1093/nar/...
#NARBreakthrough #SyntheticBiology #AlphaFold2 #GenomeEditing #ComputationalBiology
AlphaFold2 predicted LSI–RDF interactions and experimentally validated several new functional pairs.
@phoeberice.bsky.social @femijohn.bsky.social
doi.org/10.1093/nar/...
#NARBreakthrough #SyntheticBiology #AlphaFold2 #GenomeEditing #ComputationalBiology
July 24, 2025 at 4:26 PM
Our work expanding the Large Serine Integrase toolkit is finally out! We used alphafold-multimer to identify likely recombination directionality factors and tested them experimentally. Much credit to fantastic postdoc Heewhan Shin.
@heewhanshin.bsky.social
#Alphafold
#MobileGeneticElements
@heewhanshin.bsky.social
#Alphafold
#MobileGeneticElements
Reposted
Finally posted - our structures that explain directionality control in large serine integrases! Read all about how these amazingly clever molecular swivels are controlled:
www.biorxiv.org/content/10.1...
Much hard work by postdoc Heewhan Shin and many thanks to collaborator Femi Olorunniji.
www.biorxiv.org/content/10.1...
Much hard work by postdoc Heewhan Shin and many thanks to collaborator Femi Olorunniji.
Structural basis of directionality control in large serine integrases
Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF...
www.biorxiv.org
January 3, 2025 at 10:02 PM
Finally posted - our structures that explain directionality control in large serine integrases! Read all about how these amazingly clever molecular swivels are controlled:
www.biorxiv.org/content/10.1...
Much hard work by postdoc Heewhan Shin and many thanks to collaborator Femi Olorunniji.
www.biorxiv.org/content/10.1...
Much hard work by postdoc Heewhan Shin and many thanks to collaborator Femi Olorunniji.