And now we snuggle with our "micro dog", Bertie, reading Magnificent Makers by @doctheagrif.bsky.social .

Blanket's an axolotl snuggie (my Halloween costume), which always makes be think of @rogerslabucd.bsky.social .

So thankful for these amazing scientists sharing their passion.

I am so lucky.

In all it's meanings.

@jeremymberg.bsky.social Any chance you can get some insider clarification?

Sorry to tangentialize this subject. An unvaccinated family with measles traveled from SC to WA state over the holidays. They went to a huge church, SeaTac ✈️, rode ferries, went to restaurants & an indoor trampoline park. As of yesterday, we now have an outbreak in my community because of it.

Thank you!!!!!

That is precisely what I'm looking for. Just need to find the ones that are the "right" size.

Any chance you happen to know some specific cell lines?

Ooohhh, good call! It's really easy to get RPE cells to make those on the coverslip. Put coverslips in complete media w FBS o/n (no cells), plate cells, grow to confluence, remove serum for 24-48hr (need to check). Some 1ary cilia stick up off the apical surface but lots are underneath the cells.

Gag!
...hunt for that *SPOT where things separate by SR...

🤦🏼‍♀️🤣

But I wonder if I can deglycerinate muscle samples from Carolina biological and stain them with phalloidin to measure sarcomeres... Have to try that at aqlm this year.

Actin is my second favorite protein. While it's great at showing that things look better, I don't find it to be convincing in showing quantitative changes to anyone who's not familiar with actin structures. Having to hunt for that shit where things separate with SR isn't as robust as I drea of.

The dye is great when used with the poroton pump inhibitor, but different cell types have different mitochondria morphology. Would love suggestions on cell type and culture conditions to maximize cristae visualization.

Fascinating!

USA person here: I was _extremely_ confused for a minute (still needed caffeine) because it's the opposite here. In the US South (East Coast) everyone says hi to you and often engage in polite banter at the store register, etc. In New England, a large percentage of folks act as if you don't exist.

They are in the middle of a high cost of living area, which is why they get enough traffic to be viable. Good service requires good employees = $$

Internet companies are greedy and there's likely aren't too many for them to choose from.