Lightnews — Scholar-powered news

Reposted by fspasqualini.bsky.social

Rita Strack @ritastrack.bsky.social · Aug 1

I have some BIG NEWS to share today!

Today I begin my next adventure as

Chief Editor of Nature Biomedical Engineering!!

(a thread) @natmethods.nature.com @natbiomedeng.nature.com @natureportfolio.nature.com

a bunch of balloons are flying in the sky .

ALT: a bunch of balloons are flying in the sky .

media.tenor.com

93 33 690

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 13

We’re hiring! 🚀 Junior Lab Tech / PhD student (cell & mol-bio) to push our Calipers tech into 3D #gastruloids. Must already have Italy work permit (sorry, can’t sponsor). Love stem cells + CRISPR? DM or send CV by 31 Aug 2025. #hiring #StemCells

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

1 2

Reposted by fspasqualini.bsky.social

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

1 4 8

Reposted by fspasqualini.bsky.social

Alessandro Bertero @berterolab.bsky.social · Jul 8

A tour de force that elevates the #CALIPERS technology to a whole new level! A must read for all iPSCs, cell cycle, and developmental biology aficionados.

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

2 4

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

Everything is open-science here, links in the pre-print, but reach out if you don't want to wait for the final repositories to be updated. www.biorxiv.org/content/10.1...

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies. ### Competing Interest Statement The authors have declared no competing interest. European Research Council, https://ror.org/0472cxd90, 852560 European Union, https://ror.org/019w4f821, 101140192 Italian Ministry of Enterprises and Made in Italy, F13C23003260003

www.biorxiv.org

2

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

By tracking rare cycling hiPSC-derived cardiomyocytes, we can monitor cell proliferation (useful in regeneration) and multinucleation and endoreplication (useful for assessing maturation) as well as structure and function integration #CCAwarePhenotyping #CCAwareRegenerativeStudies #MethodInACellLine

1 1 3

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

New light-sheet capture of 4-color hiPSC-cardioids reveals compaction before calcium transients initiation. 90 % exit cycle by d4.5 → maturation on cue. #CCAwarePhenotyping #MethodInACellLine

1 2

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

Safe-harbor genome editing ensures expression across differentiated lineages, and WTC-11 genetic background means full compatibility with the
@alleninstitute.org Cell Collection: the largest repository of GFP/RFP-based hiPSC reporter lines www.allencell.org/cell-catalog...

1 2

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

WTC11-based hiPSCs reporter lines. We provide a 4-color CALIPERS hiPSC line with sensors for actin, calcium, and the cell cycle, as well as a blank canvas line to add your favorite sensors. CALIPERS is a #MethodInACellLine!

1 2

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

Phase-locked motility
Each nucleus gets a %-cell-cycle score that rides with its mask and trajectory. Compare shape or speed by cycle progress, not frame time. Watch HaCaTs sprint through physical space and cell cycle (CC) phases! #PhaseLockedMotility #CCAwarePhenotyping

1 2

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

Proposed solution: To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.

1 1 5

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

Problem: Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors.

1 2

fspasqualini.bsky.social @fspasqualini.bsky.social · Jul 8

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

1 4 8

Reposted by fspasqualini.bsky.social

Florian Jug @florianjug.bsky.social · Feb 11

The preprint is now available! Check it out... and... if you want to repost, why not 👇the post down here👇, so others get the bioRxiv link right away? Oh, and... 𝐭𝐡𝐚𝐧𝐤𝐬! 🙏

doi.org/10.1101/2025...

bsky.app/profile/flor...

Florian Jug @florianjug.bsky.social · Feb 11

🔬🎤 As pre-announced a few days ago... please let us proudly present to you: 𝑴𝒊𝒄𝒓𝒐𝕊𝒑𝒍𝒊𝒕 - your ticket to imaging more, imaging more gentle, and/or imaging more efficient. 🔬

doi.org/10.1101/2025...

Like ❤️, repost 🔂, and most importantly... please send feedback ✉️ our way! 🙏

3 9 21

Reposted by fspasqualini.bsky.social

Florian Jug @florianjug.bsky.social · Feb 8

Immagine you could image two cellular structures in the same fluorescent channel and still reliably get them separated afterwards…
What would you do with this?

Now… what would you do if that also worked with 4 structures at once? 👇 #MicroSplit #preview🧵

Schematic overview on how MicroSpit operates. It can split superimposed structures in fluorescent image channels by using a suitably trained AI.

Concrete example for how MicroSplit unmixes four superimposed structures.

6 38 110

Reposted by fspasqualini.bsky.social

Florian Jug @florianjug.bsky.social · Feb 11

🔬🎤 As pre-announced a few days ago... please let us proudly present to you: 𝑴𝒊𝒄𝒓𝒐𝕊𝒑𝒍𝒊𝒕 - your ticket to imaging more, imaging more gentle, and/or imaging more efficient. 🔬

doi.org/10.1101/2025...

Like ❤️, repost 🔂, and most importantly... please send feedback ✉️ our way! 🙏

2 35 85

fspasqualini.bsky.social @fspasqualini.bsky.social · Feb 10

You are too kind! And we definitely couldn't have done this without the help of your lab and @florianjug.bsky.social's!

2

fspasqualini.bsky.social @fspasqualini.bsky.social · Feb 9

And @berterolab.bsky.social joined us here on Bluesky, give him and his amazing lab a Follow!

fspasqualini.bsky.social @fspasqualini.bsky.social · Jan 17

To turn OFF Fucciplex in hiPSC-CM, we expressed it under the EF1a promoter in hiPSCs. Then, we worked with
Ale Bertero in Turin to perform scRNAseq in cardiac organoids to show progressive downregulation along the cardiac myocyte lineage.

2

Reposted by fspasqualini.bsky.social

European Research Council (ERC) @erc.europa.eu · Feb 7

Hello, Bluesky! ☀️

We fund frontier research in Europe—bold ideas, unexpected discoveries and science that shapes the future. So it’s only fitting we’ve landed here. Sorry for being late.

Follow us for updates on ERC funding, research policy, and our grantees' discoveries.

42 380 1.1K

Reposted by fspasqualini.bsky.social

Bede Portz @ysptsps.bsky.social · Jan 28

www.nature.com/articles/s41...

A genome-wide atlas of human cell morphology - Nature Methods

An optical pooled cell profiling platform (PERISCOPE) based on Cell Painting and optical sequencing of molecular barcodes was used to develop the first unbiased genome-wide morphology-based perturbati...

www.nature.com

2 9

Reposted by fspasqualini.bsky.social

fspasqualini.bsky.social @fspasqualini.bsky.social · Jan 17

HYDRA supports fluorescence microscopy, enabling cell-cycle and cytoskeletal analyses with FUCCIplex sensors using CALIPERS (biorxiv.org/content/10.1...). From cell proliferation to mitotic spindle dynamics, this method works with cutting-edge imaging and image-analysis modalities.

1 1 2

Reposted by fspasqualini.bsky.social

fspasqualini.bsky.social @fspasqualini.bsky.social · Jan 23

And works with organoids and light sheet microscopy as well!

4 6

fspasqualini.bsky.social @fspasqualini.bsky.social · Jan 23

And works with organoids and light sheet microscopy as well!

4 6

Reposted by fspasqualini.bsky.social

Rebecca Mosher @rm0sher.bsky.social · Jan 22

I'm happy to announce a postdoctoral position is available in my lab. We have lots of great data to analyze with respect to transposons & epigenetics and cool biological questions to answer.
Please reskeet and share.

Joining | The Mosher Lab

www.mosherlab.com

3 74 71

Reposted by fspasqualini.bsky.social

Jennifer Waters @jencwaters.bsky.social · Jan 21

Applications for our Quantitative Imaging: From Acquisition to Analysis 2025 course (Mar 24-Apr 8) are due on Jan 31! Come immerse yourself in microscopy and image analysis with me, @talleylambert.bsky.social, @bethcimini.bsky.social, @florianjug.bsky.social & Hunter Elliott! Please RT

27 45