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George Campbell @geobellward.bsky.social · 2d

Yes, that fixed it. I can open each one and Save as LUT and back into the LUTs folder. They now show up in LUTs manager as anything else would.

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George Campbell @geobellward.bsky.social · 2d

They don't. If I open them in Notepad, they (256 rows of 3 8-bit values) don't seem to have the same format as the other .lut files, so that must be the issue, although I can use them in all other applications. As you've made some LUTs, is there an explanation of how to format a .lut file properly?

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George Campbell @geobellward.bsky.social · 2d

one more excellent feature: set it up to run on Fiji launch by catching the command in the macro Recorder

Edit -> Options -> Startup
run("Channels and Contrast");
🥳

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George Campbell @geobellward.bsky.social · 2d

Hmm, I have a couple of manually added LUTs in my Fiji. Is there a way to get them to be read into the LUT manager also? They are in the luts folder and show up in the Lookup Table menu and in the NeuroCytoLUTs menu (after a few tweaks).

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George Campbell @geobellward.bsky.social · 2d

Yes, I was wondering how the estimated description and colors were assigned (and what happens if someone had additional LUTs). Cool, thanks!

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George Campbell @geobellward.bsky.social · 2d

one question... how is the LUTs finder annotated/organized?

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George Campbell @geobellward.bsky.social · 2d

Very cool features so far! My favorite features : grayscale preview, color groupings, favorite LUTs, merge/split/reorder menus within easy reach, perceptual uniformity scale

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George Campbell @geobellward.bsky.social · 4d

Can’t wait to give this a whirl on Monday!

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Reposted by George Campbell

Kevin Terretaz @kwolbachia.bsky.social · 4d

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

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Reposted by George Campbell

BioVoxxel (Jan Brocher) @biovoxxel.de · 14d

Bio-Imaging with Shakespeare: pseudocoloring (LUTs) — reveal hidden imaging issues and highlight important data.

✔ Spot noise, shading & saturation
✔ Map data for spatial insight
✔ Make color accessible to all
✔ Why to use a calibration bar

Full guide, tools & tutorial inside 👇
buff.ly/8x7Shiq

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George Campbell @geobellward.bsky.social · 10d

The Zeiss 63X/1.15 WI 0.6mm WD is our workhorse right now.

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Reposted by George Campbell

Rita Strack @ritastrack.bsky.social · 10d

#GEF25 the expansion microscopy community would benefit from high NA, long working distance water objectives. Who's working on these? What's out there?

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Reposted by George Campbell

Nadja M. Hümpfer @nadjahuempf.bsky.social · 10d

Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF

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Reposted by George Campbell

Christophe 🔬 L @christlet.bsky.social · 12d

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

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George Campbell @geobellward.bsky.social · 11d

Awesome, I am impressed with the flatness of the bottom surface of the cell. I see curvature of the bottom surface in some my samples, and I'm trying to identify the cause. Looks like I need to try some U-ExM!

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George Campbell @geobellward.bsky.social · 11d

Animated z-stack of fixed and expanded cell(s). Perhaps I should add the height per frame to clarify.

George Campbell @geobellward.bsky.social · 11d

I am using a TREx-1000 protocol (for ~4X expansion via 1000ug/mL bis-acrylamide) with a few small adjustments.

The actin stain is pre-gelation staining via Chrometra's modified phallodin probes (based on the TRITON probes from the Hofkens lab)

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George Campbell @geobellward.bsky.social · 11d

Thank you for correcting me, I truly appreciate it and wish that I could edit the post. Although mitosis is not my background (obviously I suppose!), I always find mitosis amazing and stop to image it. I will be more careful with my descriptions in the future.

George Campbell @geobellward.bsky.social · 12d

I am also very curious. The cell looks to be in wonderful shape!

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George Campbell @geobellward.bsky.social · 12d

Meanwhile, I'll add the info here:

Fibroblasts prepared for ExM and imaged on a spinning disk confocal microscope.
DNA in purple (BIOP-ElectricIndigo)
Outer mitochondrial membrane in blue (BIOP-Azure)
F-actin in grayscale
alpha-tubulin in orange (BIOP-Amber)
LUTs selected via NeuroCytoLUTs plugin

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George Campbell @geobellward.bsky.social · 12d

Actually, I don't see how to read the alt-text on a video. Anyone know?

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George Campbell @geobellward.bsky.social · 12d

Bringing some more ExM to #FluorescenceFriday with what appears to be a frustrated cell division in fibroblasts.

More details in Alt-text.

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Reposted by George Campbell

Nicolas Denans @nicolasdenans.bsky.social · 19d

The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

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Reposted by George Campbell

Beth Cimini 🔬💻📊 @bethcimini.bsky.social · 15d

Halfway to I2K is BACK, friends of all kinds! Last year, 650 people attended 30+ TOTALLY FREE image analysis workshops of all kinds, across many timezones.

If you make image analysis software and want to teach it, workshop submissions are open now! We'd love to have your tool highlighted.

BioImaging North America @bioimagingna.bsky.social · 19d

#HappyFluorescenceFriday!

#microscopycommunity- want to learn open source image analysis or share your knowledge to help others? We’ve got a FREE virtual workshop Nov 17-19! Now accepting workshop session applications!

Learn more & sign up: buff.ly/esGIotD

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George Campbell @geobellward.bsky.social · 18d

Amazing view, and so Talley. Jealous of those shoes but watch your ankles!

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