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Michalis Gounis

@gounismichalis.bsky.social

160 followers 230 following 27 posts

Postdoc @Ivaskalab.bsky.social @BioscienceTurku (previously @CRUK-SI.bsky.social) with an interest in tumour microenvironment, metabolism and invasion #tumourmicroenviroment#metastasis

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Michalis Gounis @gounismichalis.bsky.social · Jun 9

1/ Beyond thrilled to see my #PhD work now published in @jcb.org
A short thread (🧵) for #metastasis #metabolism #ExtracellularVesicles (EVs) aficionados

Journal of Cell Biology @jcb.org · Jun 9

@gounismichalis.bsky.social‬ et al. show that cells that seed early lung #metastasis in #BreastCancer have altered #metabolism. These metabolic alterations lead them to release extracellular vesicles which engender a microenvironment conducive to invasive growth. rupress.org/jcb/article/...

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Reposted by Michalis Gounis

Cassie Clarke @cassiejclarke.bsky.social · Jul 23

Delighted to share that our work “Uridine Phosphorylase-1 supports metastasis by altering immune and extracellular matrix landscapes” was published today in @emboreports.org, describing how neutrophil metabolism influences niche priming to support metastasis (1/14) www.embopress.org/doi/full/10....

Uridine phosphorylase-1 supports metastasis by altering immune and extracellular matrix landscapes | EMBO reports

imageimageMetastatic primary tumours upregulate Upp1 in neutrophils, which elevates circulating uracil. UPP1-expressing neutrophils accumulate in the lung where they inhibit CD8+ T cells, promote fibr...

www.embopress.org

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Reposted by Michalis Gounis

Journal of Cell Science @jcellsci.bsky.social · Jul 16

Rijuta Lamba, Alexandra Naba @nabalabuic.bsky.social, @valerioizzi.bsky.social and team present MatriCom, a single-cell RNA-sequencing data mining tool to infer cell–ECM interactions.
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...
#OpenAccess

Figure showing the building MatriComDB, an omnibus database of matrisome protein interactions.

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Reposted by Michalis Gounis

Joseph Hetmanski @mankie06.bsky.social · Jul 14

Pleased that our latest paper is now out in @jcellsci.bsky.social, in collaboration with @drmcjones.bsky.social and others from Manchester and Plymouth: Differential roles of cyclin–CDK1 complexes in cell migration and invasion url: journals.biologists.com/jcs/article/...

Differential roles of cyclin–CDK1 complexes in cell migration and invasion

Highlighted Article: Cyclin–CDK1 complexes have a previously undiscovered role in regulating normal and cancer cell migration in 3D matrices.

journals.biologists.com

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Reposted by Michalis Gounis

Ivaska Lab @ivaskalab.bsky.social · Jul 16

Latest from Ivaska Lab ▶️ Niklas Jäntti et al delve deep into the roles of EPLINα/β on integrin trafficking and breast cancer outcomes!

Congratulations to all the authors!

Check the paper out here: www.sciencedirect.com/science/arti...

EPLINα controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration

Epithelial protein lost in neoplasm (EPLIN), an actin-binding protein, has been described as both a tumor promoter and tumor suppressor in different c…

www.sciencedirect.com

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Reposted by Michalis Gounis

Johanna Ivaska @johannaivaska.bsky.social · Jul 16

Excited to share our most recent study on #integrintraffic #cancer #migration #focaladhesion by @ivaskalab.bsky.social @drpaulinamoreno.bsky.social Niklas Jäntti et al www.sciencedirect.com/science/arti...

EPLINα controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration

Epithelial protein lost in neoplasm (EPLIN), an actin-binding protein, has been described as both a tumor promoter and tumor suppressor in different c…

www.sciencedirect.com

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Reposted by Michalis Gounis

MRC Laboratory of Molecular Biology @mrclmb.bsky.social · Jun 23

Great opportunity for a Research Support Officer to join @cellbiol-mrclmb.bsky.social, supporting @deriverylab.bsky.social in their studies of the molecular mechanisms behind receptor sorting in endosomes.
More info: www.nature.com/naturecareer...
Apply by 10 JUL
#ScienceJobs

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Reposted by Michalis Gounis

Guillaume Jacquemet @guijacquemet.bsky.social · Jun 23

Are you looking for a postdoc?
I will be looking for 2-4 people after the summer to join the lab !!
Do not hesitate to reach out and disseminate !!

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Reposted by Michalis Gounis

Nature Cell Biology @natcellbio.nature.com · Jun 12

☕Conway et al. show that #integrin β1 phosphorylation is regulated through balanced activities of tyrosine kinases, such as Src and Arg, and phosphatases, such as PTP-PEST and Shp2, facilitating invadopodia formation and invasion of #BreastCancer cells.
bit.ly/4n4v3JS

Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells - Nature Cell Biology

Conway et al. show that integrin β1 phosphorylation is regulated through balanced activities of tyrosine kinases, such as Src and Arg, and phosphatases, such as PTP-PEST and Shp2, facilitating invadop...

bit.ly

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Michalis Gounis @gounismichalis.bsky.social · Jun 12

Congratulations @louisefets.bsky.social and team, beautiful work!! 👏👏
www.biorxiv.org/content/10.1...

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Michalis Gounis @gounismichalis.bsky.social · Jun 11

Thank you so much Kirsteen 😊

Michalis Gounis @gounismichalis.bsky.social · Jun 10

Vassili, thank you very much for your kind words 😊

Michalis Gounis @gounismichalis.bsky.social · Jun 9

Martha, thank you for your support and kind words 😊

Michalis Gounis @gounismichalis.bsky.social · Jun 9

@cassiejclarke.bsky.social, thank you very much for the wonderful supervision 😊

Michalis Gounis @gounismichalis.bsky.social · Jun 9

Thank you very much Paulina 😄

Michalis Gounis @gounismichalis.bsky.social · Jun 9

Thank you very much Guillaume 😊

Michalis Gounis @gounismichalis.bsky.social · Jun 9

10/ Massive thanks to @cassiejclarke.bsky.social and Jim for the wonderful supervision and @cancerresearchuk.org for the funding.
@uofglasgow.bsky.social @uofgcancersciences.bsky.social

Hope you enjoy reading!!

a cartoon dog is reading a book in a bed

ALT: a cartoon dog is reading a book in a bed

media.tenor.com

Michalis Gounis @gounismichalis.bsky.social · Jun 9

9/ Grateful to everyone that made this possible, especially members of the Norman lab for their great contribution @vanecitav.bsky.social @ruhi-d.bsky.social and members of the @ivaskalab.bsky.social for the unwavering support.

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Michalis Gounis @gounismichalis.bsky.social · Jun 9

8/ Profoundly thankful to D. Sumpton, E. Shokry, S.Tardito for their metabolic expertise, K. Blyth and @louisemitchell21.bsky.social for their invaluable insights into the in vivo models. All the core facilities and services @cruk-si.bsky.social for the amazing work.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

7/ Interestingly, disruption of Rab27s opposes EV release from primary tumour cells, but not from micrometastatic cells. Lastly, we show that the switch to nSM2-dependent EV release enables micrometastatic cells to influence #ECM deposition by fibroblasts to foster an invasive #microenvironment.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

6/ Following the observation that ceramides are enriched in micrometastatic cells, we disrupted the expression of n. sphingomyelinases known to influence EV release. We found that reduction of nSM2, but not nSM1, significantly reduces ceramide levels and EV release from micrometastatic cells.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

5/ In addition to their altered polar metabolism, we uncover that micrometastatic cells display elevated levels of distinct #ceramide species, which are also enriched in their EVs and are present at significantly higher levels in the plasma of metastatic breast cancer patients.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

4/ Given the importance of #EVs in metastatic niche priming, we sought to examine the relationship of EV release from micrometastatic cells to their altered metabolism. We found that decreased ability to synthesise glutathione is associated with increased EV release.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

3/We further demonstrate that proline and glutathione pathways compete for glutamine-derived carbons in micrometastatic cells. The adoption of this metabolic profile, in which proline and glutathione levels are, respectively, elevated and suppressed, is a feature unique to micrometastatic cells.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

2/ We generated isogenic cells from primary mammary tumours and their corresponding lung micrometastases. We show that micrometastatic cells increase proline production and circulating proline is significantly elevated in mice and patients with metastatic disease.

Michalis Gounis @gounismichalis.bsky.social · Jun 9

a cartoon dog is reading a book in a bed

ALT: a cartoon dog is reading a book in a bed

media.tenor.com