What if at the end of one of @christlet.bsky.social next talks a ready to play saxophone randomly appears... (scribbles plan)

Happy #FluorescenceFriday. Christophe Leterrier⭐in #TheMicroscopists and reflects on the
make-or-break moment at the end of his postdoc
when he came close to leaving academia before securing his CNRS position
and how these experiences shape the way he mentors others.
Stream: bit.ly/microscopist...

🌟 Announcing Prof. Arrate Muñoz-Barrutia as a Plenary Speaker at #SPAOM2025!
She’s leading the way in 🧠 AI + 🔬 bioimage analysis to transform how we detect and treat disease.

Don’t miss her in “Hot Topics in Bioimage Analysis and AI” 🤖📊

#Microscopy #AIinScience #LifeSciences #WomenInSTEM

🔬 Got new data that’s hot off the microscope?
SPAOM is accepting late-breaking abstracts for poster presentations!
🗓️ Deadline: Oct 13
📍 Present your work at the premier microscopy meeting of the Iberian community.

Submit here ➡️ spaom2025.org/registration/
#SPAOM #Microscopy #CallForAbstracts

🌟 We’re thrilled to have Esther González-Almela at #SPAOM2025!

She’s a Marie Skłodowska-Curie Fellow at CNB-CSIC, exploring how viruses manipulate nuclear architecture using advanced imaging tools 🔬

Her talk will be one to watch!

#Microscopy #Virology

Looking for your next adventure? Want to be our colleague? #NIMSB is a new institute in medical systems biology recruiting group leaders.
It's a sister institute to ours (@itqbnova.bsky.social), offering excellent opportunities for collaboration. Come be our scientific neighbours!

Passionate about #SystemsBiology and ready to lead your own research group?
Join us at the forefront of discovery — apply now to be a #GroupLeader at the NOVA Institute for Medical Systems Biology, in beautiful Lisbon, PT!

Appl. by Oct 8:
www.nature.com/naturecareer...

#ScienceJobs #JobOffer

He's either really short or the microscope is really big, get the man a ladder, stat

Check the full preprint and its movies for more details: www.biorxiv.org/content/10.1...
Congrats @wiesner-t.bsky.social and the whole team!
@inp-marseille.bsky.social @ncis-marseille.bsky.social @univ-amu.fr @cnrsbiologie.bsky.social @agencerecherche.bsky.social @neuromarseille.bsky.social 11/11

And we confirmed this by performing 2-color TIRF imaging of VAMP2-pHluorin and clathrin-mCherry along axons, showing that over 5 minutes, very few exocytic sites were also sites of clathrin pit formation 10/11

So are exocytic events taking place in the same spectrin clearings as clathrin pits formation? Postdoc Christopher helped extending our correlative approach to 2-color SMLM on the @abbelight.bsky.social scope, showing that exocytic sites are distinct from clathrin pit-containing clearings 9/11

Please note the cool detection and annotation of spectrin nanoscale periodicity in these experiments (yellow stripes) - we used the recently published SReD approach that was developed by @afonsomendes92.bsky.social and @henriqueslab.bsky.social: www.nature.com/articles/s41... 8/11

Are exocytic events happening in holes of the spectrin scaffold? To answer this question, we developed live-cell / SMLM correlative imaging to link exocytic sites to their nanoscale environment. Yes, exocytosis along the axon shaft occurs in areas devoid of the submembrane spectrin scaffold! 7/11

In our neuronal cultures, we could visualise spontaneous exocytosis of VAMP2-pHluorin containing vesicles not only at presynapses (in magenta below), but also along the axon shaft itself (outside of the pink blobs) 5/11

Seeing exocytosis is harder than endocytosis: faster, no clathrin pits visible in fixed cells, requiring live-cell imaging! We used TIRF to image the release of vesicles containing VAMP2-pHluorin along axons, and pHusion from @stephgupton.bsky.social's lab (doi.org/10.1242/jcs....) to detect it 4/11

What about exocytosis? It would also need access to the plasma membrane - it happens a lot at presynapses that happen to be devoid of the periodic submembrane scaffold… Can exocytosis happen along the axon shaft at sites determined by the actin-spectrin scaffold? 3/11

Together with @stefvass.bsky.social, we previously found that the dense submembrane axonal scaffold is regulating the formation of clathrin-coated pits along the proximal axon, by forming circular ”clearings” that allow access to the plasma membrane: www.science.org/doi/10.1126/... 2/11

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11

Awesomeness from the NeuroCyto lab (kudos @wiesner-t.bsky.social for great story)! They found exocytosis in "holes" within the axon's spectrin skeleton, separate from endocytosis sites. Our team mapped structural patterns and disruptions (via #SReD). Thanks for including us @christlet.bsky.social

The plugin can also be run in separate steps for debugging and to use different segmentation algorithms for registration. (9/9)

www.youtube.com/watch?v=cypD...

💻 You can directly try out CLEM-Reg for yourself with our @napari.org plugin where we also include a sample benchmark dataset.

Installation instructions are here 👉 github.com/krentzd/napa... (8/9)

www.youtube.com/watch?v=ud3z...

Super happy to see CLEM-Reg out in @natmethods.nature.com! A short thread on the algorithm behind it and how to try it out for yourself🧵(1/9)

www.nature.com/articles/s41...

Awesome to see this out. Thank you for bringing us in the journey @em-stp.bsky.social and @martinjones.bsky.social. Keep rocking @danielkrentzel.bsky.social!

LLM-agent never skips leg day