Excited to share our latest preprint. Work led by PhD student @jordancjwilson.bsky.social with @profmarciniak.bsky.social, @villungerlab.bsky.social & our @erc.europa.eu partners @jcornlab.bsky.social & @loizoulab.bsky.social, that sheds light on the action of WEE1 inhibitors. bit.ly/4bDpxZl

Do you want to know about the invisible secrets of #HDR during #CRISPR genome editing? Check out our preprint at www.biorxiv.org/content/10.1.... New methods, crazy biology, and theft from the genome! From PhD student @charlesyeh.bsky.social

I’m still learning Bluesky, so now tagging people I mis-tagged. Sorry! @charlesyeh.bsky.social @albertomarin.bsky.social @fedeteloni.bsky.social @gerlichlab.bsky.social

If you’re interested in how loop-extruding cohesin drives the homology search during HR, check out also this new preprint from albertomarin.bsky.social in the Taekjip Ha lab (doi.org/10.1101/2025...)

Curious about how cohesin guides homology search via loops & sister chromatid linkages? Check out this new preprint by fedeteloni.bsky.social & gerlichlab.bsky.social! 👉 tinyurl.com/DSBcohesin

This project was driven by amazing graduate student charlesyeh.bsky.social, with lots of amazing collaborators over the years.

Altogether, proximity is key and search normally stays around the DSB. When it comes choosing a donor, only these sequences are candidates.

During genome editing, exogenous donor DNAs soak up homology search during HDR. Even if they have no sequence relationship to the cut site at all. They actually steal search from the genome!

Only 3D-nearby those sequences that are searched (i.e., candidates) are chosen as HDR donors! Donors too far away are never seen and never used.

Search is constrained by chromatin conformation. Check out the related preprints from fedeteloni.bsky.social
and albertomarin.bsky.social for a much deeper dive (see later in thread).

We call this "RaPID-seq". It unveils a new dimension of DNA repair, telling us what is searched during HDR. In other words, what are the candidates?

New #preprint from the lab! Do you want to see the invisible? So did we! When DNA genomes get broken, cells somehow find related sequences to fix the break. But how do they find it? We developed a way to look at sequence *search*, not just sequence usage.
doi.org/10.1101/2025...

This project was led by
@matthiasmuhar.bsky.social, @jakobfarnung.bsky.social Martina Cernakova, and Raphael Hofmann.

Thanks to amazing collaborators in the Schulman, Jinek, and Jessberger labs. And most of all to Jeff Bode's lab, who has been with this every step of the way. @micharapelab.bsky.social wrote a News and Views that explains our work far better than I ever could. www.nature.com/articles/d41...

Cells are filled with toxic stuff that damages healthy proteins. Is that garbage just left to rot on the curb? No way! Ubiquitin ligases have evolved to recognize chemical damage and clean it up! www.nature.com/articles/s41...

In Berkeley: www.darkcarnival.com

Join Helle D. Ulrich & experts this April to explore DNA replication errors, breaks, repair, and cancer! Submit abstracts and scholarships by Jan. 28 (11:59pm MST)! keysym.us/KSDNARep25

#KSDNARep25 #DNAreplicationgaps @cantorlab.bsky.social @albertociccia.bsky.social @labvincenzo.bsky.social