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Johan Duchêne

@johanduchene.bsky.social

2.9K followers 3.1K following 190 posts

Dad, Husband & Scientist | Immunology, Inflammation, Chemokines & Atypical ChemoKine Receptors (ACKRs) | Research & Meta-Research | University of Munich (LMU) Molecular Inflammation Lab https://duchene.ipek-research.com

duchene.ipek-research.com

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Johan Duchêne @johanduchene.bsky.social · Apr 30

Antibodies don't work.

NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific.

Only 4 of 14 tested antibodies detected NLRP3 specifically.

Antibodies must be validated before use.

Full report by @ycharos.bsky.social : zenodo.org/records/1162...

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Johan Duchêne @johanduchene.bsky.social · 9d

Here is the Antibody Characterization Report for E3 ubiquitinprotein ligase parkin (Parkin)
zenodo.org/records/5747...

More reports are found here: ycharos.com/data/

Antibody Characterization Report for E3 ubiquitin-protein ligase parkin (Parkin)

Head-to-head comparison of available commercial antibodies against E3 ubiquitin-protein ligase parkin (Parkin) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The following s...

zenodo.org

Johan Duchêne @johanduchene.bsky.social · 9d

Indeed !

Johan Duchêne @johanduchene.bsky.social · 9d

Yes the website is super new and will be updated every month with new antibodies.

Johan Duchêne @johanduchene.bsky.social · 9d

Sorry to hear that OGA website does not work at Memorial Sloan Kettering.
Maybe try this ycharos.com
All data are available here too, but it is more tedious as you would need to download a report for each protein.

Accueil / Home

YCharOS Inc. YCharOS Inc. est une entreprise canadienne d’intérêt public, dont la mission est de caractériser les anticorps commerciaux disponibles pour chaque protéine humaine, tout en suivant les pr...

ycharos.com

Johan Duchêne @johanduchene.bsky.social · 9d

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Johan Duchêne @johanduchene.bsky.social · 9d

PARKIN labels damaged mitochondria for recycling
No PARKIN → no cleanup → neurodegeneration
Mutations in PARK2 are common in Parkinson’s disease

Accurate detection of PARKIN is crucial

Problem:
The most used antibodies are not selective
In contrast, some very specific antibodies are not used

Parkin antibody screening by immunoblot.
Lysates of SH-SY5Y (WT and PRKN KO) were prepared and 50 μg of protein were processed for immunoblot with the indicated Parkin antibodies. The Ponceau stained transfers of each blot are shown. Antibody dilution used: 702785 at 1/200; 711820 at 1/200; 39-0900 at 1/500; ab77924 at 1/200; ab15954 at 1/500; 14060-1-AP at 1/1000; MAB1438 at 1/250; MAB14381 at 1/500; GTX39745 at 1/1000; GTX65811 at 1/1000. Predicted band size: 52 kDa.

Which antibody should you choose then?

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Johan Duchêne @johanduchene.bsky.social · 9d

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Johan Duchêne @johanduchene.bsky.social · 9d

⚠️ Half of commercial antibodies miss their target!
So how do you pick the right one for your experiment?

Here’s the solution 👉
Antibody characterization data (all tested in KO cells) are now available at:
🔗 onlygoodantibodies.co.uk

Tested by @ycharos.bsky.social & @oga-community.bsky.social

OGA - Only Good Antibodies Community

onlygoodantibodies.co.uk

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Reposted by Johan Duchêne

The Conversation U.S. @us.theconversation.com · Sep 8

The myth of scientific objectivity hides the truth that every experiment is influenced by cultural values, assumptions and beliefs – and that actually hurts the search for truth

Analysis:
buff.ly/KcLU0bE
🧪 🩺

Scientific objectivity is a myth – cultural values and beliefs always influence science and the people who do it

Cultural ideas are inextricably entwined with the people who do science, the questions they ask, the assumptions they hold and the conclusions they land on.

buff.ly

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Reposted by Johan Duchêne

Adrian Liston @labliston.bsky.social · Sep 3

We've also switched from mostly transgenic mouse strains to AAV/retrovirus "transgenes". It cut mouse costs by >50%, and makes our work faster and more responsive to reviewer requests. AAVs can even be used to produce depleting antibodies.

www.sciencedirect.com/science/arti...

Durable lymphocyte subset elimination upon a single dose of AAV-delivered depletion antibody dissects immune control of chronic viral infection

To interrogate the role of specific immune cells in infection, cancer, and autoimmunity, immunologists commonly use monoclonal depletion antibodies (d…

www.sciencedirect.com

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Reposted by Johan Duchêne

Adrian Liston @labliston.bsky.social · Sep 3

You can also save money by cutting-costs and preventing waste. We do a low of flow cytometry, and retitrated antibodies for overnight staining, reducing costs ~100-fold while improving signal-to-noise and reproducibility

currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Do more with Less: Improving High Parameter Cytometry Through Overnight Staining

Recent advances in flow cytometry have allowed high-dimensional characterization of biological phenomena, enabling breakthroughs in a multitude of fields. Despite the appreciation of the unique prope...

currentprotocols.onlinelibrary.wiley.com

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Reposted by Johan Duchêne

World Conferences on Research Integrity @wcrifoundation.bsky.social · Jun 27

Abstract submission and travel grant application for the 9th World Conference on Research Integrity are open. Please consider to submit an abstract and to reshare this message. lnkd.in/eJ9hmaRs

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Reposted by Johan Duchêne

Reese Richardson @reeserichardson.bsky.social · Aug 4

Today, our article "The entities enabling scientific fraud at scale are large, resilient, and growing rapidly" is finally published in PNAS. I hope that it proves to be a wake-up-call for the whole scientific community.

reeserichardson.blog/2025/08/04/a...

A do-or-die moment for the scientific enterprise

Reflecting on our paper “The entities enabling scientific fraud at scale are large, resilient, and growing rapidly”

reeserichardson.blog

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Reposted by Johan Duchêne

Adam Braithwaite @braithwaiteat.bsky.social · Jul 3

Stimulating immune cells in vitro is a common experimental lab model. We profiled 150K blood immune cells treated with 11 different stimuli to compare the effects. The data are freely available for researchers. See the preprint for our findings and to access the data 🧪 doi.org/10.1101/2025...

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Johan Duchêne @johanduchene.bsky.social · Jul 7

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Johan Duchêne @johanduchene.bsky.social · Jul 7

A comparison of mouse vs human tumor microenvironments shows a major difference.

Mouse TMEs have more macrophages, while human TMEs have more T cells.

Chemokine expression patterns are also different.

Learn more: www.biorxiv.org/content/10.1...

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Johan Duchêne @johanduchene.bsky.social · Jun 27

#FluorescenceFriday & Erythroblast

Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue).

Captured via Correlative Light & Electron Microscopy by
@jemimaburden.bsky.social

Learn more: scim.ag/4kTxFZz
@antalpedro.bsky.social
@jffoley.bsky.social
@science.org

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Reposted by Johan Duchêne

Antal Rot @antalpedro.bsky.social · Jun 24

In our science.org/doi/10.1126/... we describe the hitherto overlooked erythroblast expression of CXCR4, which do not migrate in response to CXCR4 ligation by CXCL12. Instead, in these cells CXCR4 localises to the nucleus and mediates alternative cell responses in maturation and triggers enucleation

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Johan Duchêne @johanduchene.bsky.social · Jun 21

With the right link:
www.science.org/doi/10.1126/...

Intracellular and nuclear CXCR4 signaling promotes terminal erythroblast differentiation and enucleation

CXCL12-stimulated intracellular and nuclear CXCR4 signaling mediates erythroblast enucleation to generate red blood cells.

www.science.org

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Johan Duchêne @johanduchene.bsky.social · Jun 21

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Johan Duchêne @johanduchene.bsky.social · Jun 21

Not all chemokine signaling is about cell migration!

In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation.

www.science.org/doi/10.1126/...

Intracellular and nuclear CXCR4 signaling promotes terminal erythroblast differentiation and enucleation

CXCL12-stimulated intracellular and nuclear CXCR4 signaling mediates erythroblast enucleation to generate red blood cells.

www.science.org

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Johan Duchêne @johanduchene.bsky.social · Jun 21

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Johan Duchêne @johanduchene.bsky.social · Jun 21

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Reposted by Johan Duchêne

Jemima Burden @jemimaburden.bsky.social · Jun 18

Yesterday.. our #CLEM with Julia Gutjahr and Antal Rot was featured in the main Science webpage....☺️ Nice to see #correlativemicroscopy and #volumeEM up front and centre.

www.science.org/doi/10.1126/...

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