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Josh Dickerson

@joshdcryoem.bsky.social

190 followers 220 following 6 posts

PostDoc in Lucas Lab at UC Berkeley. Formerly PhD student in Russo group at MRC-LMB and Master's student in Garman group at Univ. Oxford.

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Josh Dickerson @joshdcryoem.bsky.social · Apr 23

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS

The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

www.pnas.org

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Reposted by Josh Dickerson

cryoEM papers @cryoempapers.bsky.social · 21d

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem

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Josh Dickerson @joshdcryoem.bsky.social · Aug 30

Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/

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Reposted by Josh Dickerson

cryoEM papers @cryoempapers.bsky.social · Aug 2

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules www.biorxiv.org/content/10.1101/2025.07.29.667395v1 #cryoem

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Reposted by Josh Dickerson

Bronwyn Lucas @bronwynalucas.bsky.social · Jul 25

@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS

The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

www.pnas.org

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Reposted by Josh Dickerson

Bronwyn Lucas @bronwynalucas.bsky.social · Jul 25

If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....

To tilt or not to tilt? Strategies for in situ cryo-EM data collection

Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

www.sciencedirect.com

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Josh Dickerson @joshdcryoem.bsky.social · Jun 28

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

To tilt or not to tilt? Strategies for in situ cryo-EM data collection

Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) and protein structure prediction have transformed our ability to resol…

www.sciencedirect.com

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Reposted by Josh Dickerson

Lewys Jones @lewysj.bsky.social · May 20

At the 2024 IMC meeting, Richard Henderson laid down a challenge. Design a #TEM single-particle #cryoEM lens with 1mm Cc at 100kV. I'm thrilled to reveal our new Arxiv preprint delivering a fully retrofittable pole-piece swap out for the @jeoleurope.bsky.social 1400 TEM.
arxiv.org/abs/2505.11458

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Reposted by Josh Dickerson

Alister Burt @alisterburt.bsky.social · May 12

In the Bay Area? Love computational cryo-EM/ET?

Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community!

Details and registration at frostbyte-cryoem.github.io

Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

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Josh Dickerson @joshdcryoem.bsky.social · Apr 23

For a shorter read, @mrclmb.bsky.social and @science.org also have articles about the helium story. www.science.org/content/arti...

Josh Dickerson @joshdcryoem.bsky.social · Apr 23

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS

The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

www.pnas.org

2 23 66

Reposted by Josh Dickerson

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Apr 6

Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1

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Reposted by Josh Dickerson

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Mar 31

In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS https://www.biorxiv.org/content/10.1101/2025.03.29.646093v1

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Reposted by Josh Dickerson

bioRxiv Biophysics @biorxiv-biophys.bsky.social · Mar 12

Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1

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Josh Dickerson @joshdcryoem.bsky.social · Jan 18

I've been wanting to see a paper using off-axis electron holography for low-dose biological imaging since the start of my PhD. Spatially resolved charge measurements👌

cryoEM papers @cryoempapers.bsky.social · Jan 18

Off-axis electron holography of unstained bacteriophages: Toward electrostatic potential measurement of biological samples pubmed.ncbi.nlm.nih.gov/39818354/

Reposted by Josh Dickerson

IUCrJ @iucrj.iucr.org · Jan 18

Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching ##3DReconstructionAndImageProcessing##SingleParticleCryoEM##Imaging@umassboston...##IUCrhttps://journals.iucr.org/paper?S2052252524011771

A new statistical metric for robust target detection in cryo-EM using 2D template matching

A novel statistical metric for 2D template matching (2DTM), the 2DTM p-value, has been developed to improve the detection of targets in cryo-EM images under various imaging and sample conditions, particularly for smaller and aspherical targets.

journals.iucr.org

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Reposted by Josh Dickerson

cryoEM papers @cryoempapers.bsky.social · Nov 22

Laser Flash Melting Cryo-EM Samples to Overcome Preferred Orientation biorxiv.org/cgi/content/short/2024.11.21.624652v1

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Josh Dickerson @joshdcryoem.bsky.social · Jan 22

It should be relatively simple with the Bio.PDB package in Biopython. It has both pdb and mmtf IO modules.

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