Jovana Kaljević ⬜️
@jovanakaljevic.bsky.social
Postdoctoral scientist | JIC, UK
PhD | de Duve institute, Belgium
Passionate about killing E. coli and watching them die under the🔬
PhD | de Duve institute, Belgium
Passionate about killing E. coli and watching them die under the🔬
Reposted by Jovana Kaljević ⬜️
The bacterial #SOSresponse unfolds in a defined temporal order, but how does this arise? @adityakamat.bsky.social @anjbadri.bsky.social &co show that intrinsic #promoter strength, modulated by #SigmaFactor usage, governs timing of SOS gene activation in Caulobacter @plosbiology.org 🧪 plos.io/4oAKf0Q
![Comparative analysis of the Caulobacter SOS response under mitomycin-C damage. Top left: Schematic summarizing the RNA-sequencing experiment. Caulobacter cells were treated with 0.25 μg/ml mitomycin-C for 20 and 40 min (Created in BioRender). Samples were collected for transcriptomic analysis before (0 min, control), and at 20 and 40 min post damage induction. Top right: Venn diagram representing the genes that meet the listed criteria: 1. Genes induced in wild type cells under MMC damage at 40 min (white circle). 2. Genes induced in ΔlexA in the absence of damage (blue circle). 3. Genes not induced in ΔrecA background under MMC damage at 40 min (gray circle). Genes fulfilling all three criteria are in the gray circle. Number of genes in each category is indicated. Bottom left: Bar graph indicating whether promoters of the shortlisted genes exhibit binding by the LexA protein as assessed from ChIP-seq analysis [10]. Bottom right: LexA ChIP-seq profile for genes belonging to the SOS response. Normalized reads (in rpm) are represented for 500 bp upstream and downstream of the gene CDS.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:5522ztebtekoor5efelihqhb/bafkreigerw7qsbl4br367qyqg3mouvx3va6s7f3pcqugpf2m24adc3wa6y@jpeg)
December 5, 2025 at 10:03 AM
The bacterial #SOSresponse unfolds in a defined temporal order, but how does this arise? @adityakamat.bsky.social @anjbadri.bsky.social &co show that intrinsic #promoter strength, modulated by #SigmaFactor usage, governs timing of SOS gene activation in Caulobacter @plosbiology.org 🧪 plos.io/4oAKf0Q
Shout-out to Antoine and our amazing collaboration!
Our study of the evolution of the ParB NTP binding domain across the Tree of Life is now published! An awesome collaboration with @tunglejic.bsky.social led by @jovanakaljevic.bsky.social at the John Ines Center. Thanks to editors and reviewers. www.pnas.org/doi/10.1073/...
www.pnas.org
December 5, 2025 at 9:19 AM
Shout-out to Antoine and our amazing collaboration!
Reposted by Jovana Kaljević ⬜️
Outstanding work from @jovanakaljevic.bsky.social as together with the team they explore the depths of ParB world🤩
On my list!
On my list!
Our preprint is now published in PNAS! This came together thanks to a great collaboration with Antoine Hocher and a strong team effort from the Le Lab. Thank you to the reviewers and to everyone who helped improve it. I hope ParB aficionados will enjoy it.
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation | PNAS
Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular
processes by cycling between active and inactive states thro...
www.pnas.org
December 4, 2025 at 10:48 PM
Outstanding work from @jovanakaljevic.bsky.social as together with the team they explore the depths of ParB world🤩
On my list!
On my list!
Reposted by Jovana Kaljević ⬜️
doi.org/10.1038/d41586…
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
November 28, 2024 at 6:14 PM
doi.org/10.1038/d41586…
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Reposted by Jovana Kaljević ⬜️
Thrilled to share our new bioRxiv preprint!✨ Huge credit to first author Yujin Chen and thanks to all co-authors. Extra special: my first paper as co-corresponding author (with Lieve Gheysen).
Check how nematodes uncouple FLS2–BSK signalling in 🌱👇
www.biorxiv.org/content/10.1...
Check how nematodes uncouple FLS2–BSK signalling in 🌱👇
www.biorxiv.org/content/10.1...
September 1, 2025 at 9:53 AM
Thrilled to share our new bioRxiv preprint!✨ Huge credit to first author Yujin Chen and thanks to all co-authors. Extra special: my first paper as co-corresponding author (with Lieve Gheysen).
Check how nematodes uncouple FLS2–BSK signalling in 🌱👇
www.biorxiv.org/content/10.1...
Check how nematodes uncouple FLS2–BSK signalling in 🌱👇
www.biorxiv.org/content/10.1...
Our preprint is now published in PNAS! This came together thanks to a great collaboration with Antoine Hocher and a strong team effort from the Le Lab. Thank you to the reviewers and to everyone who helped improve it. I hope ParB aficionados will enjoy it.
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation | PNAS
Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular
processes by cycling between active and inactive states thro...
www.pnas.org
December 4, 2025 at 8:10 PM
Our preprint is now published in PNAS! This came together thanks to a great collaboration with Antoine Hocher and a strong team effort from the Le Lab. Thank you to the reviewers and to everyone who helped improve it. I hope ParB aficionados will enjoy it.
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Reposted by Jovana Kaljević ⬜️
Happy to finally show our story of the connection between nucleoid conformation, transcription & predation in our favorite predator Bdellovibrio bacteriovorus. 🧵 1/n
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 26, 2025 at 9:09 AM
Happy to finally show our story of the connection between nucleoid conformation, transcription & predation in our favorite predator Bdellovibrio bacteriovorus. 🧵 1/n
Thrilled to see this work out. I enjoyed our collaboration @antoinehocher and loved working on this project. Congrats to the entire team! @tunglejic.bsky.social
new preprint from our group & Antoine Hocher: www.biorxiv.org/content/10.1...
A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.
A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.
Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation
Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states through nucleotide binding and hydrolysis. These mechanis...
www.biorxiv.org
October 11, 2025 at 1:12 PM
Thrilled to see this work out. I enjoyed our collaboration @antoinehocher and loved working on this project. Congrats to the entire team! @tunglejic.bsky.social
Reposted by Jovana Kaljević ⬜️
ParB NTPase fold might:
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
October 11, 2025 at 11:36 AM
ParB NTPase fold might:
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
This was fun!
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
academic.oup.com
October 6, 2025 at 8:44 PM
This was fun!
Reposted by Jovana Kaljević ⬜️
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 25, 2025 at 7:12 AM
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
doi.org/10.1038/d41586…
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
November 28, 2024 at 6:14 PM
doi.org/10.1038/d41586…
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social