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JungmannLab @jungmannlab.bsky.social · 6d

www.nature.com/articles/s41...

Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution - Nature Communications

By combining left- and right-handed DNA-PAINT probes, Unterauer et al. achieve simple, robust, and highly multiplexed super-resolution. They show 13-plex neuronal maps, revealing nanoscale organization of cytoskeleton, organelles, and synapses.

www.nature.com

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JungmannLab @jungmannlab.bsky.social · 6d

Thanks to all who made this possible! @eduardunterauer.bsky.social @evaschentarra.bsky.social @ipachmayr.bsky.social @taishatashrin.bsky.social Jisoo Kwon Sebastian Strauss, @jekristina.bsky.social @rafalkowalew.bsky.social @opazo.bsky.social @forna.bsky.social @lumasullo.bsky.social (6/6)

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JungmannLab @jungmannlab.bsky.social · 6d

Within this neuronal atlas we can reveal the three synapse classes, excitatory, inhibitory and the recently discovered mixed synapse. Organelle imaging of Peroxisomes (Pmp70) and the Golgi Apparatus (Golga5) reveals rare contact sides and even fused particles. (5/6)

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JungmannLab @jungmannlab.bsky.social · 6d

To show the power of the technique, we acquired a 13-plex 200 x 200 µm2 neuronal atlas in 3D. With this atlas we map the interaction architecture of three neurons, resolving organelles, cytoskeleton, vesicles and synapses at single-protein resolution. (4/6)

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JungmannLab @jungmannlab.bsky.social · 6d

We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)

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JungmannLab @jungmannlab.bsky.social · 6d

The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)

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JungmannLab @jungmannlab.bsky.social · 6d

Highly efficient 12-color multiplexing with speed-optimized DNA-PAINT. We are excited to share our latest paper in @natcomms.nature.com, using left-handed DNA to extend speed-optimized DNA-PAINT to 12 targets in a simple and straightforward way! 🧬👈🚀https://www.nature.com/articles/s41467-025-64228-x

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Reposted by JungmannLab

Heilemann Lab @heilemannlab.bsky.social · Aug 27

Next on stage is Eduard Unterauer @eduardunterauer.bsky.social from @jungmannlab.bsky.social reporting spatial proteomics with DNA PAINT #SMLMS2025

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JungmannLab @jungmannlab.bsky.social · Jul 30

We're excited that the study is now out in Nature Nanotechnology @natnano.nature.com www.nature.com/articles/s41...

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Reposted by JungmannLab

Nature Nanotechnology @natnano.nature.com · Jul 28

New paper online:

Ångström-resolution imaging of cell-surface glycans.

The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.

#Glycotime

www.nature.com/articles/s41...

Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology

By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

www.nature.com

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JungmannLab @jungmannlab.bsky.social · Jul 28

Congratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6)
Read the full story here: www.nature.com/articles/s41...

Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI - Nature Communications

The nanoscale organization of the antigen-antibody complexes influences the therapeutic action of monoclonal antibodies. Here, the authors present a multi-target 3D RESI imaging assay for the nanomete...

www.nature.com

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JungmannLab @jungmannlab.bsky.social · Jul 28

The shift from Type II to Type I function reveals a structure–function continuum for anti-CD20 antibodies, showing that receptor arrangements dictate mechanism of action. RESI provides a platform for structure-guided antibody development, applicable far beyond CD20. (5/6)

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JungmannLab @jungmannlab.bsky.social · Jul 28

We showed a direct link between CD20 oligomerization and function by investigating OBZ-based T-cell engagers (TCEs). An increased IgG flexibility in the 2+1 TCE format lead to increased CD20 tetramerization, without higher-order clustering, resulting in a reduction of direct cytotoxicity. (4/6)

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JungmannLab @jungmannlab.bsky.social · Jul 28

In contrast, Type II antibodies like Obinutuzumab and H299 induced limited oligomerization to dimers, trimers and tetramers, consistent with their role in promoting direct tumor cell death rather than complement activation. (3/6)

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JungmannLab @jungmannlab.bsky.social · Jul 28

By imaging intact cells, we could see these therapeutic antibodies in action: Type I antibodies like Rituximab and Ofatumumab formed extended chains of CD20 hexamers or larger, creating platforms compatible with complement protein binding, mediating cancer cell killing. (2/6)

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JungmannLab @jungmannlab.bsky.social · Jul 28

Ever wondered what happens when therapeutic antibodies bind to cancer cells? In our latest study, we used multiplexed 3D-RESI to directly visualize how anti-CD20 antibodies interact with their receptors, revealing their precise arrangement at single-protein resolution. (1/6)

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Reposted by JungmannLab

Max Planck Institute of Biochemistry @mpibiochem.bsky.social · Jul 3

Congratulations to Ralf on your election as a new EMBO member:

❕Original press release from @embo.org : www.embo.org/press-releas...

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JungmannLab @jungmannlab.bsky.social · Jun 14

Great science, great company and stunning views at our Lab retreat on Schloss Ringberg 🏰🧬🔬. Big thanks to our guests Sabrina Simoncelli, Sebastian Kobold, Thomas Schlichthärle, @massivephotonics.bsky.social & students from the @lfmilles.bsky.social and @mlsb-borgwardt.bsky.social Labs for joining!