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Reposted by Laura de Rooij

Aging Science News @agingbiology.bsky.social · Aug 23

Uncovering the transcriptional hallmarks of endothelial cell aging via integrated single-cell analysis
www.biorxiv.org/content/10.1...

Uncovering the transcriptional hallmarks of endothelial cell aging via integrated single-cell analysis

Endothelial cells (ECs) are critical regulators of vascular function and exhibit specialized, organ-specific roles across tissues. During aging, these cells become dysfunctional, resulting in increase...

www.biorxiv.org

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Laura de Rooij @lderooij.bsky.social · Aug 19

We hope our atlas can go beyond and become a framework to explore how #vascular #aging unfolds across the human body. Explore the data yourself at haeca.derooijlab.org, and check out the rest of the story here: biorxiv.org/content/10.1.... And importantly, let us know what you think! 9/9

haeca.derooijlab.org

Laura de Rooij @lderooij.bsky.social · Aug 19

Led by Sarah Dobner, this is the very first derooijlab.org production, and we want to thank all lab members and collaborators for their contributions, our host institution @cemm.oeaw.ac.at, as well as our funders. 8/9

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Laura de Rooij @lderooij.bsky.social · Aug 19

5️⃣ Lastly, by integrating the atlas data with in vitro models of induced EC senescence, we uncovered a core set of genes linked to CDKN1A/p21 expression. We validated this across models, tissues and species, to further our understanding of senescence regulation in ECs. 7/9

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Laura de Rooij @lderooij.bsky.social · Aug 19

4️⃣ Age-associated ECM remodeling signatures were linked to increased activity of #mechanotransduction regulators YAP/TAZ and TEAD, particularly in skin, lung, and liver. Immunofluorescence confirmed increased nuclear YAP in aged human skin ECs. 6/9

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Laura de Rooij @lderooij.bsky.social · Aug 19

3️⃣ Venous aging is different:
Vein ECs lose expression of multiple angiogenic regulators with age, while increasing genes linked to extracellular matrix (ECM) remodeling and altered glycosylation, hinting at effects on #angiogenesis, barrier function, and immune trafficking. 5/9

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Laura de Rooij @lderooij.bsky.social · Aug 19

2️⃣ Inflammaging is everywhere:
Across tissues, aging ECs showed increased expression of inflammatory signaling, immune cell adhesion markers, and #senescence-associated factors. 4/9

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Laura de Rooij @lderooij.bsky.social · Aug 19

1️⃣ A human vascular “Rosetta Stone”:
We identified conserved molecular markers for each endothelial subtype across tissues. These can be used to pinpoint arterial, venous, capillary, or lymphatic ECs in any healthy human tissue context. 3/9

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Laura de Rooij @lderooij.bsky.social · Aug 19

By integrating and harmonizing endothelial cells (ECs) from 34 publicly available #single-cell and single-nucleus #RNAseq datasets, we found 🧵(2/9):

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Laura de Rooij @lderooij.bsky.social · Aug 19

🚨Preprint alert!🚨What happens to our blood vessels as we age? To begin answering this at the transcriptome level, we built the Human Aging Endothelial Cell Atlas (HAECA), profiling >375,000 endothelial cells from 12 tissues across adulthood. www.biorxiv.org/content/10.1... (1/9)

| bioRxiv

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

www.biorxiv.org

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Reposted by Laura de Rooij

Rae Radford @raeradford.bsky.social · Jul 19

#BookSky

550 7.8K 39K

Reposted by Laura de Rooij

Manlio De Domenico @manlius.bsky.social · Jul 19

"our results indicate that academia pays a price by focusing attention and resources on superstars"

Nice to see this supported by data analysis, now.

H/T: @cxdig.bsky.social

www.nature.com/articles/s41...

The innovation trade-off: how following superstars shapes academic novelty - Humanities and Social Sciences Communications

Humanities and Social Sciences Communications - The innovation trade-off: how following superstars shapes academic novelty

www.nature.com

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Reposted by Laura de Rooij

CeMM @cemm.oeaw.ac.at · Jun 24

🔬 As we near the end of #EquationsForLife, today we spotlight @ar-abdelfattah.bsky.social's Group!
They use the #ReactionDiffussion equation to characterize the process of tissue organization and maintenance in physiology, and how disease disrupts it.
🔎 bit.ly/4ef2Pbe

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Reposted by Laura de Rooij

CeMM @cemm.oeaw.ac.at · Jun 16

We kicked off the week with Roger Kamm (@mitdeptofbe.bsky.social) for a #CeMMinar on brain neurovascular models & its applications to modeling drug & toxin transport. Thanks to Roger for the great insights & hosts @lderooij.bsky.social & @ar-abdelfattah.bsky.social

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Reposted by Laura de Rooij

CeMM @cemm.oeaw.ac.at · Jun 5

A very insightful Impromptu Seminar today at #CeMM by Kumar Sharma (@uthealthsa.bsky.social), hosted by our PI @ar-abdelfattah.bsky.social! His talk on spatial mass spectrometry & precision therapeutics offered powerful insights into disease mechanisms and inter-organ crosstalk. Thank you, Kumar!

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Reposted by Laura de Rooij

Project MOTION @crgmotion.bsky.social · May 16

🌐 Introducing MOTION WorkPackage 2 by @reibergerthomas.bsky.social 🌐

In this particular WP the team is focusing on decompensated cirrhosis..
#liver #PortalHypertension #clinicaltrails #dACLD

🔽 Find out more in the video below 📷🔽

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Reposted by Laura de Rooij

CeMM @cemm.oeaw.ac.at · May 5

Let’s kick off the week with #EquationsForLife! @lderooij.bsky.social’s group studies how #aging affects endothelial cells in blood vessels. Using #MurraysLaw & 3D “vessel-on-a-chip” models, they aim to decode & combat cardiovascular aging.
🔍 tinyurl.com/CeMMRR2024-d...
#CeMMResearchReport2024

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Laura de Rooij @lderooij.bsky.social · Apr 23

🚨 Exciting Opportunity! 🚨
Join us on June 9th in Brussels for a satellite conference to the 4th MPS World Summit, where we will address advancements & challenges in #aging research using #microphysiological systems and #organoids.
Sign up here: lnkd.in/dCu_yCBr

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Laura de Rooij @lderooij.bsky.social · Feb 23

All of this in a super supportive research environment in one of the world's most liveable cities!

For further details and to apply, please follow the link below: lnkd.in/d_K4NdJA

Check out our website for more info about our group and ongoing projects: derooijlab.org

CeMM hiring Laboratory Technician in Vascular Heterogeneity (m/f/d) in Vienna, Austria | LinkedIn

Posted 11:40:28 AM. The laboratory of Laura de Rooij at CeMM, the Research Center for Molecular Medicine of the…See this and similar jobs on LinkedIn.

lnkd.in

Laura de Rooij @lderooij.bsky.social · Feb 23

🚨Job alert🚨! We are looking for a highly motivated research technician to join us in our quest to dissect the transcriptomic make-up and function of endothelial cells across the human body, using cutting-edge omics technologies, clinical samples, and vessel-on-chip models. Please share & apply! 👇

CeMM hiring Laboratory Technician in Vascular Heterogeneity (m/f/d) in Vienna, Austria | LinkedIn

Posted 11:40:28 AM. The laboratory of Laura de Rooij at CeMM, the Research Center for Molecular Medicine of the…See this and similar jobs on LinkedIn.

lnkd.in

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Laura de Rooij @lderooij.bsky.social · Jan 6

Only two weeks left to apply, don't miss out!! 👇

Laura de Rooij @lderooij.bsky.social · Dec 19

Looking for a fully funded PhD position in wonderful Vienna? The CeMM PhD program is now open for applications! 🎓 🥼 🧫

We are seeking passionate and driven students interested in helping us understand #endothelial cell heterogeneity in both healthy and pathological #aging. See below for more info 👇

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Reposted by Laura de Rooij

Hongkui Zeng @hongkuizeng.bsky.social · Jan 2

Excited to share our single-cell study on aging mouse brain! We found 2,449 cell-type specific age-DE genes, and an aging hotspot in specific hypothalamic cell types involved in energy homeostasis, which show both decreased neuronal function and increased immune response. doi.org/10.1038/s415...

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Laura de Rooij @lderooij.bsky.social · Dec 19

Learn more about our research: derooijlab.org
Find out how to apply: cemm.at/phd-program

Laura de Rooij @lderooij.bsky.social · Dec 19

We make use of state-of-the-art #omics technologies and human in vitro models, including exciting #vesselonchip systems. Wet & dry lab enthusiasts are thus both very welcome to apply! Relevant links 👇

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Laura de Rooij @lderooij.bsky.social · Dec 19

Looking for a fully funded PhD position in wonderful Vienna? The CeMM PhD program is now open for applications! 🎓 🥼 🧫

We are seeking passionate and driven students interested in helping us understand #endothelial cell heterogeneity in both healthy and pathological #aging. See below for more info 👇

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