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Anthony Leung @leunglab.bsky.social · 8d

As the Chair of Innovation Translation Council, venturing beyond lab science at the Bloomberg American Health Summit 2025.
A lot to absorb — and much to reflect on about scaling public health innovation to reach millions.

Grateful to Josh Sharfstein for making it possible to attend 🙏

Reposted by Anthony Leung

Johns Hopkins Department of Biochemistry and Molecular Biology @johnshopkinsbmb.bsky.social · 22d

The @johnshopkinssph.bsky.social Office of Research launched the Innovation Translation Council, chaired by BMB Professor Anthony Leung (@leunglab.bsky.social)! The ITC will connect researchers at the School with resources to translate their innovative research into community impacts. Learn more ⤵️

research@BSPH Launches Innovation Translation Council | Johns Hopkins Bloomberg School of Public Health

publichealth.jhu.edu

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Anthony Leung @leunglab.bsky.social · Jun 3

Congrats, Kevin!!

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Anthony Leung @leunglab.bsky.social · May 10

Thanks Waters, SCIEX, Seer, Proteintech and Avantor for sponsorship!!

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Anthony Leung @leunglab.bsky.social · May 10

What a fantastic day of science at FCBIS! @friedlab.bsky.social and I had a blast co-organizing this exciting dive into the chemistry-biology interface—great conversations, amazing energy, and a standout keynote by
@edwardmarcotte.bsky.social. Thanks to all who joined us!

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Reposted by Anthony Leung

Review Commons @reviewcommons.org · Apr 30

Interferon function couples immune signaling to the ubiquitin–proteasome system.

Study from the @leunglab.bsky.social @johnshopkinsbmb.bsky.social

🆕 #preprintpeerreview @biorxivpreprint.bsky.social

Referee reports and author's response letter available here

--> doi.org/10.1101/2024...

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Reposted by Anthony Leung

Hartmut Vodermaier @hvodermaier.bsky.social · Apr 8

Great to get to publish another one on the emerging connections between ADP-ribosylation, immune signaling, ubiquitin and protein aggregation/degradation

Anthony Leung @leunglab.bsky.social · Apr 8

🥳 Out now @embojournal.org!
Interferon induces a new class of p62 bodies, with #antiviral & #immunotherapy implications.
p62 is usually linked to #autophagy — but not here.
These are driven by PARP14-mediated ADP-ribosylation & rely on #ubiquitin #proteasome system. www.embopress.org/doi/full/10....

Interferon-induced PARP14-mediated ADP-ribosylation in p62 bodies requires the ubiquitin-proteasome system | The EMBO Journal

imageimageInterferon induces the formation of ADP-ribosylation (ADPr)-enriched p62 bodies, requiring PARP14 and p62. Unlike canonical p62 bodies, these ADPr-enriched structures are independent of auto...

www.embopress.org

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Anthony Leung @leunglab.bsky.social · Apr 8

Thanks Mike - also we saw ubiquitin involvement here - it will be interesting to see whether it related to your nice work on protein-conjugated ADP-ribose directly linked to ubiquitin. Need another nice bakery to chat more :-)

doi.org/10.1038/s443...

Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal

imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...

doi.org

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Anthony Leung @leunglab.bsky.social · Apr 8

For #condensate folks 👀
Our study tackles a key gap: how condensate composition is regulated.
These p62 bodies require PARP14—but it’s not just presence that matters.
It’s PARP14’s enzymatic activity that drives formation.
In fact, more PARP14 without activity ≠ condensation.

Anthony Leung @leunglab.bsky.social · Apr 8

Why it matters 👇
p62 bodies are key hubs for protein degradation & immune signaling—long linked to #cancer & #neurodegeneration.
Our study shows interferon + PARP14-driven ADP-ribosylation rewires their formation, revealing a new immune-regulated condensate class.

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Anthony Leung @leunglab.bsky.social · Apr 8

🥳 Out now @embojournal.org!
Interferon induces a new class of p62 bodies, with #antiviral & #immunotherapy implications.
p62 is usually linked to #autophagy — but not here.
These are driven by PARP14-mediated ADP-ribosylation & rely on #ubiquitin #proteasome system. www.embopress.org/doi/full/10....

Interferon-induced PARP14-mediated ADP-ribosylation in p62 bodies requires the ubiquitin-proteasome system | The EMBO Journal

imageimageInterferon induces the formation of ADP-ribosylation (ADPr)-enriched p62 bodies, requiring PARP14 and p62. Unlike canonical p62 bodies, these ADPr-enriched structures are independent of auto...

www.embopress.org

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Anthony Leung @leunglab.bsky.social · Apr 1

In the midst of funding uncertainty in D.C., our lab took a moment to see the cherry blossoms.

We’re not naive—we know the challenges.

But we believe in science, in community, and in each other.

We’re in this together.

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Anthony Leung @leunglab.bsky.social · Mar 24

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Anthony Leung @leunglab.bsky.social · Mar 13

Great work from Dr. Isabel Uribe, based on her PhD thesis research! 🎓 With collaborations across three top proteomics teams led by Chan Hyun Na, Benjamin A. Garcia @garcialabms.bsky.social & her co-mentor, Benjamin C. Orsburn @proteomicsnews.bsky.social. 🎉

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Anthony Leung @leunglab.bsky.social · Mar 13

New Paper💡PARP enzymes regulate ADP-ribosylation, a protein modification linked to cancer, infections & neurodegeneration. But it comes with two forms—MARylation (single) & PARylation (multiple). Meet dELTA-MS, a mass spec method that identifies both in one experiment!
pubs.acs.org/doi/full/10....

dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms

ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. Little is known about the specific contribut...

pubs.acs.org

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Reposted by Anthony Leung

Trends in Biochemical Sciences @cp-trendsbiochem.bsky.social · Mar 6

Be sure to also read about:
☘️ #Nrf2 activity regulation by #UbLigases (Albena Dinkova-Kostova et al)
☘️ roles of structured domains in #biomolecularcondensates (@jerelleaj.bsky.social et al)
☘️ PARPs and ADP-ribosylation-mediated #biomolecularcondensates (@leunglab.bsky.social et al)

...

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Reposted by Anthony Leung

Mike Cohen @michaelnadbio.bsky.social · Feb 25

I'm super excited to share our study in collaboration with @jnpruneda.bsky.social, published today in @embojournal.org! We show that PARPs are modified with a non-canonical ester-linked mono-ADP-ribosylated ubiquitin species for the first time in cells: www.embopress.org/doi/full/10....

Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal

imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...

www.embopress.org

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Anthony Leung @leunglab.bsky.social · Feb 22

Celebrating 14 years of science, teamwork, and resilience! To mark our lab’s anniversary, we traded pipettes for pottery—turns out shaping clay is just as therapeutic as shaping experiments. With NIH funding uncertainties, a little calm goes a long way. Grateful for this team! #LabLife

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Anthony Leung @leunglab.bsky.social · Feb 12

Excited to share our new review on biomolecular condensates mediated by PARPs and ADP-ribosylation @ Trends in Biochemical Sciences!

Despite the uncertainty, science must march forward. Grateful for the support that makes discovery possible. #FundScience #KeepScienceStrong

Trends in Biochemical Sciences @cp-trendsbiochem.bsky.social · Feb 10

Available online - the Review "PARPs and ADP-ribosylation-mediated #biomolecularcondensates: determinants, dynamics, and disease implications" from @leunglab.bsky.social and colleagues.

#phaseseparation #proteincondensation #proteinaggregation

Free access at: authors.elsevier.com/a/1kZlO3S6Gf...

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Reposted by Anthony Leung

Jeremy Berg @jeremymberg.bsky.social · Feb 8

A Primer on Indirect Cost Rates

a cartoon says hey everybody an old man 's talking while bart simpson looks on

ALT: a cartoon says hey everybody an old man 's talking while bart simpson looks on

media.tenor.com

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Anthony Leung @leunglab.bsky.social · Jan 17

Thanks for highlighting our work, Marcin!

Marcin J. Suskiewicz @msuskiewicz.bsky.social · Jan 16

I read 2 condensate papers today, the 1st consistent with the focus on folded domains. It discussed a protein called PARP13 that contributes to growth by having folded domains capable of various interactions (binding to poly(ADP-ribose), homodimerisation, binding to RNA) - the first being key.

Regulation of stress granule maturation and dynamics by poly(ADP-ribose) interaction with PARP13 - Nature Communications

Stress granules, cellular structures essential for stress response, require poly(ADP-ribose) as a multivalent scaffold. Here, the authors show that disrupting poly(ADP-ribose) binding to PARP13 alters...

www.nature.com

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Anthony Leung @leunglab.bsky.social · Jan 15

With ~2,000 PAR-binding proteins identified, many enriched in condensates and bind RNA, our work provides a new mechanistic framework for the role of PAR and its crosstalk with the chemically similar counterpart, RNA, in condensate biology

Anthony Leung @leunglab.bsky.social · Jan 15

Notably, PARP13 mutations reduce stress granule fluidity, resembling changes seen in neurodegeneration. These mutations could serve as a valuable tool to study how altered granule dynamics might increase toxicity in neurodegenerative disease models.

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Anthony Leung @leunglab.bsky.social · Jan 15

We propose PARP13—the only inactive PARP in stress granules—acts as a stress-dependent “amplifier” of multivalency, making new connections between protein-RNA and RNA-RNA interactions to stress-induced PARylated proteins, facilitating the maturation of stress granules.

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Anthony Leung @leunglab.bsky.social · Jan 15

🔥 Most studies on condensates focus on their formation—but what about their maturation? Our research reveals PARP13 binds poly(ADP-ribose) (PAR) to drive stress granule maturation.

Mutate the binding sites? Granules fragment like crazy!

Read the full story here:
www.nature.com/articles/s41...

Regulation of stress granule maturation and dynamics by poly(ADP-ribose) interaction with PARP13 - Nature Communications

Stress granules, cellular structures essential for stress response, require poly(ADP-ribose) as a multivalent scaffold. Here, the authors show that disrupting poly(ADP-ribose) binding to PARP13 alters...

www.nature.com

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