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Marie Winz

@mariewinz.bsky.social

170 followers 260 following 13 posts

RNA biologist at Johannes Gutenberg University Mainz, Germany: co-translational quality control - RNA modification - and beyond. Lab url: ak-winz.pharmazie.uni-mainz.de

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Reposted by Marie Winz

Kusterlab @kusterlab.bsky.social · 29d

New preprint: We isolate peptide–RNA photo-crosslinks with tunable RNA chains from living cells for mass spec. This maps over 4,700 crosslinking sites across 744 proteins and offers the first glimpse into the RNA sequences in crosslinks by MS. Read here: doi.org/10.1101/2025...

Peptide-RNA photo-crosslinks with tunable RNA chain map protein-RNA interfaces

Photo-crosslinking mass spectrometry enables the identification of protein-RNA interactions in living cells, pinpointing interaction interfaces at single-amino acid resolution. However, current isolat...

Reposted by Marie Winz

Marie Winz @mariewinz.bsky.social · 29d

Thrilled to announce our #preprint on a new factor in the last line of defense in #translation quality control out on #bioRXiv! Spearheaded by fantastic PhD student @kaushikiyer.bsky.social, supported by Chloé Walter, Alina Kraft, Max Müller and Lena Tittel.

bioRxivpreprint @biorxivpreprint.bsky.social · Sep 6

Jlp2 is an RQC complex-independent release factor acting on aberrant peptidyl-tRNA, protecting cells against translation elongation stress https://www.biorxiv.org/content/10.1101/2025.09.04.673968v1

Marie Winz @mariewinz.bsky.social · 29d

Curiously, Jlp2 acts not only on peptidyl-tRNA on large ribosomal subunits, but also on full monosomes. However, we also show that Jlp2 requires ribosome splitting for activity, suggesting that the monosomes represent re-assembled subunits. Read our preprint for full story!

Marie Winz @mariewinz.bsky.social · 29d

Instead, we find that Jlp2 acts as a release factor enabling peptide release from ribosome-bound peptidyl-tRNA. We show that Jlp2 acts independently of the RQC complex and of other, known or expected release factors. Through peptide release, Jlp2 maintains cellular fitness in translation stress.

Marie Winz @mariewinz.bsky.social · 29d

We identify yeast Jlp2 as a translation quality control factor that regulates CAT tailing upon RQC dysfunction, counteracting CAT tail-dependent protein aggregation. However, NOT by physically competing with or influencing abundance or function of Rqc2, the factor that catalyzes CAT tailing.

Marie Winz @mariewinz.bsky.social · 29d

Stalled or collided ribosomes are split during quality control, leaving aberrant nascent peptides conjugated to tRNA bound to large ribosomal subunits. Those peptides are targeted by #RQC, and ubiquitinated and/or CAT tailed to facilitate degradation. If RQC fails, who comes to the rescue?

Marie Winz @mariewinz.bsky.social · 29d

Thrilled to announce our #preprint on a new factor in the last line of defense in #translation quality control out on #bioRXiv! Spearheaded by fantastic PhD student @kaushikiyer.bsky.social, supported by Chloé Walter, Alina Kraft, Max Müller and Lena Tittel.

bioRxivpreprint @biorxivpreprint.bsky.social · Sep 6

Jlp2 is an RQC complex-independent release factor acting on aberrant peptidyl-tRNA, protecting cells against translation elongation stress https://www.biorxiv.org/content/10.1101/2025.09.04.673968v1

Reposted by Marie Winz

Clementine Delan-Forino @clemdf.bsky.social · Aug 18

Thrilled to share my first last-author paper, just out in #mSystems! A work about #RBPs, #RNA and #sporulation in #subtilis.
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...

Remodeling of RNA-binding proteome and RNA-mediated regulation as a new layer of control of sporulation | mSystems

Understanding how bacteria survive extreme conditions is key to tackling challenges in health, food safety, and industry. This study reveals a previously unexplored layer of control in Bacillus subtil...

journals.asm.org

Marie Winz @mariewinz.bsky.social · Sep 5

#proudPI moment yesterday, seeing my PhD student @kaushikiyer.bsky.social ace his talk at #EMBLProtein conference, presenting his work on new translation quality control factor Jlp2. Really did a great job holding up our lab's flag!!! 😄

Reposted by Marie Winz

Eva Kowalinski @kowaeva.bsky.social · Jul 17

Postdoc positions in structural biology of RNA processing complexes.

An ERC-funded position will dissect pre-mRNA processing pathways in the infective organism Trypanosoma brucei. An ANR-funded position will characterise large complexes in RNA modification.

Details: tinyurl.com/postdoc-RNA-...

Postdoc in Structural Biology of RNA processing complexes

Postdoctoral positions in structural biology of macromolecular complexes are available in the laboratory of Dr. Eva Kowalinski at the EMBL Grenoble, France. We are looking for highly motivated and amb...

Marie Winz @mariewinz.bsky.social · May 23

Congrats!

Marie Winz @mariewinz.bsky.social · Apr 17

Congrats!!!

Reposted by Marie Winz

Clementine Delan-Forino @clemdf.bsky.social · Apr 15

🚨 New preprint! 🚨
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social

Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation

Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has ...

www.biorxiv.org

Reposted by Marie Winz

Molecular Cell @cp-molcell.bsky.social · Mar 26

Online Now: UbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains Online now:

UbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains

Ubiquitin chains determine the fates of their modified proteins, including proteasomal degradation. Kiss et al. present UbiREAD, a technology to monitor cellular degradation and deubiquitination at high temporal resolution after intracellular delivery of ubiquitinated proteins. This reveals a degradation code for ubiquitin chains varying by linkage, length, and topology.

Marie Winz @mariewinz.bsky.social · Feb 25

4. Backtracked RNA can be polyadenylated (AAA) on the 3' end by the TRAMP complex. We speculate that AAA-marked nascent RNA could either be degraded or trigger reverse torpedo termination.

Although this work focuses on RNAPI, many findings apply broadly to RNAPs

Marie Winz @mariewinz.bsky.social · Feb 25

3. We proposed an alternative model for torpedo termination, where a 5'→3' exonuclease trims the nascent RNA to RNAP boundaries, followed by backtracking-mediated cleavage.

Marie Winz @mariewinz.bsky.social · Feb 25

2. We uncovered multiple mechanisms contributing to RNAPI transcription termination, including road-block termination, end-nucleolytic cleavage, and Rat1 torpedo termination.

Marie Winz @mariewinz.bsky.social · Feb 25

Summary by Tomasz:
Some key takeaways:
1. The first experimental evidence supporting the model of immobilized RNAPs and spinning DNA—validated by experimental confirmation of theoretical predictions.

Marie Winz @mariewinz.bsky.social · Feb 25

Paper alert 🚨 Check out our new paper about RNA polymerase I termination! Great collaboration with
Liz Petfalski, Katarzyna Grelewska-Nowotko and David Tollervey!
online yesterday in @CellReports! It took a while but finally come to life! t.co/5RPpxAhRvU
It was a pleasure to contribute!

https://www.cell.com/cell-reports/fulltext/S2211-1247(25)00096-8