Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.

'Innovation does not occur in a vacuum. It requires raw materials: the findings of basic research. We are currently burning through a stockpile of knowledge accumulated during a more enlightened era of public investment, while allocating insufficient resources for replenishing it.'

Well … we are both definitely in there

Yes, especially for flat things like cell monolayers or your hand 😜

No, this was me messing around independently. Only deskew; no rotation. It is actually surprising how little difference it makes.

Dilute more than recommended. At least 1:4000. Probably even more for the SPY-DNA dyes.

... and a projection perpendicular to the light sheet, so not truly a view from the top but at 38 (or 52) degrees ... this is still giving me a headache.

SPY-555 DNA and SPY-650 FastActX ...

well ... maybe, if someone invites me 🤪. I am realizing that one full scan of three channels of a relatively flat volume is 1GB ... this is going to present some challenges for time-lapse.

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

I’m heartened to see this.

Yep. That’s the question. Tempted to test with nanocages because it it is really this bright and stable would be very useful… however, @erin-rod.bsky.social, is this thing a dimer?

Intriguing …

Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social

Vaccine samples from the 1880s in Louis Pasteur’s laboratory in Arbois … Vaccines work!

Bluetorial: Non-Competitive Renewal (Continuation) Awards

Normally, multiyear NIH grants are paid with awards each year, pending administratively reviewed progress reports. “NIH” has been slow in making these awards. This has led to $1.83 B being withheld from institutions through February 2025.

Got stentor … thanks @wallaceucsf.bsky.social

At the North Bay Science Discovery Day today in Santa Rosa

Crowd is forming in SF #standupforscience

WHERE WILL YOU BE ON MARCH 7TH!?

Head to www.standupforscience2025.org/local-event-information/ or the link in our bio to find your closest event—or add one if you're hosting one!

#standupforscience2025

“The idea is that the public has the right to know who’s giving advice to the federal government and when they’re meeting,” said Jeremy Berg, a genetic scientist who has overseen NIH funding in the past.

1/3

[I am a genetic scientist in the sense that I have genes, but I am a biochemist]

New York Times article now up on Federal Register stuff.

www.nytimes.com/2025/02/21/s...

1/n

Thank you! It is really f***** up.