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www.protocols.io @protocolsio.bsky.social · 2d

3. The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management
www.protocols.io/view/the-spa...

The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management

This protocol standardizes the classroom administration, scoring, and data management of the Spatial Thinking Ability Test (STAT-24), a category-balanced instrument for secondar...

www.protocols.io

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www.protocols.io @protocolsio.bsky.social · 2d

2. Water and sediment sampling in the sea to discover marine bacteria
www.protocols.io/view/water-a...

Water and sediment sampling in the sea to discover marine bacteria

This protocol aims to show the strategy for obtaining water and sediment samples on board an oceanographic vessel at sea. The samples obtained can be used to identify marine bac...

www.protocols.io

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www.protocols.io @protocolsio.bsky.social · 2d

1. Intracellular Metabolite Extraction, Peak Detection with El-MAVEN and Quantification Using Python V.2
www.protocols.io/view/intrace...

Intracellular Metabolite Extraction, Peak Detection with El-MAVEN and Quantification Using Python

This protocol outlines a comprehensive workflow for intracellular metabolite analysis from microbial cultures. It begins with the rapid quenching and extraction of metabolites u...

www.protocols.io

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www.protocols.io @protocolsio.bsky.social · 2d

Explore our new featured protocols protocols.io/welcome

Bring structure to your research - protocols.io

A secure platform for developing and sharing reproducible methods.

protocols.io

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Lenny Teytelman @lteytelman.bsky.social · 6d

I've been dreaming about this for a decade and it's now live!

"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...

Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io

Research is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t...

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Michael Meers @mpmeers.bsky.social · 16d

We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1

Plate CUT&Tag (Meers Lab version)

This protocol describes a method for carrying out CUT&Tag-Direct in 96 well microplates for high-throughput processing of samples by a single operator with only standard lab...

dx.doi.org

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Allen Institute @alleninstitute.org · 21d

Copy our protocols!

📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22...

🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc...

#OpenScienceWeek

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Emma Ganley @emmaganley.bsky.social · Aug 14

We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager

See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...

Image of the job posting text as it appears on LinkedIn

🌟 We’re Hiring: Associate / Scientific Engagement Manager
📍 Location: New York or Jersey City (Hybrid)

I'm excited to share that we're looking for an Associate Scientific Engagement Manager to join the protocols.io team at Springer Nature

This is a fantastic opportunity for someone to lead outreach and engagement efforts across the global research community—hosting webinars, collaborating with institutions, and supporting researchers in adopting protocols.io to improve reproducibility and accelerate discovery.

🔬 If you have a background in research, love science communication, and thrive in collaborative environments, we’d love to hear from you!

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Magnetic Resonance News @nmr900.bsky.social · Jul 31

Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲

Network for Advanced NMR Materials SSNMR Data Acquisition Protocols

The NAN Materials SSNMR Data Acquisition Protocols collection is part of the NAN Materials SSNMR Knowledgebase; a collection of resources, training and educational material, pul...

www.protocols.io

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 29

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

Bring structure to your research - protocols.io

A secure platform for developing and sharing reproducible methods.

protocols.io

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Anya Suppermpool @asupp.bsky.social · Jul 22

Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org [email protected] papers.
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...

Re-using pipette tips for gel loading

Gel loading tips can be reused for most applications; there’s REALLY no need to throw them away after a single use. In our labs, we routinely reuse tips when loading gels, onl...

www.protocols.io

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Yi Yang @ohrp.bsky.social · Jul 22

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Bas...

Culture-based isolation of bacteriophages from wastewater relies on a limited set of laboratory‐cultivable host strains, causing it to recover only a small fraction of the tru...

www.protocols.io

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 23

Happy to answer any and all questions about this work. We'd love to see more people try it.
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...

CRAFTseq

Genetic studies have identified thousands of individual disease-associated non-coding alleles, but identification of the causal alleles and their functions remain critical bottl...

www.protocols.io

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 23

I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.

www.nature.com/articles/s41...

Precisely defining disease variant effects in CRISPR-edited single cells - Nature

A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants o...

www.nature.com

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www.protocols.io @protocolsio.bsky.social · Jul 8

Thank you for sharing your methods knowledge publicly!

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Anneke_P @plants-genes-bugs.bsky.social · Jul 4

The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪

dx.doi.org/10.17504/pro...

Microplate Beta Glucan Assay

This assay measures the mixed linkage beta glucan content of cereal grain flour through specific, enzyme-catalysed, step-wise hydrolysis of the polysaccharide to glucose and qua...

dx.doi.org

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Dr. Bryan H. Juarez (He/They) @bhjuarez.bsky.social · Jun 26

🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let...

The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!

Non-lethal 3-D imaging techniques for estimating tadpole morphology

The majority of protocols for imaging biological specimens are lethal and this raises both ethical and scientific concerns. One major limitation of most imaging protocols is tha...

www.protocols.io

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Marta Niedzicka @theplantvirologist.bsky.social · Jun 11

Last month we published our protocol for detection of beet yellows virus. Here it is!

RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...

RT-LAMP for BYV detection

This protocol describes the process of detecting beet yellows virus (BYV) in plant material utilising a quick and easy colorimetric assay. The starting point is total RNA extrac...

www.protocols.io

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sylvain Brisse @sylvainbrisse.bsky.social · Jun 6

qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io

Identification of Corynebacteria of the diphtheriae Species Complex and detection of the diphtheria toxin g...

This protocol describes a quadruplex real-time PCR (Dip4plex), performed with the Qiagen QuantiNova™ Multiplex PCR Kit, for simultaneous identification of Corynebacteria of th...

www.protocols.io

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Emma Ganley @emmaganley.bsky.social · Jun 10

Happy to be in Brno for the #CTLS2025 conference from the Core Technologies for Life Sciences society. I'll be talking about protocols.io tomorrow morning in the Core Facilities and Open Science session

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www.protocols.io @protocolsio.bsky.social · Jun 6

Spotlight interview with Maria Diehl regarding her PLOS ONE Lab Protocol paper: "Modifying the platform-mediated avoidance task: A new protocol to study active avoidance within a social context in rats." @realdiehllab.bsky.social

Protocol Spotlight with Maria Diehl

YouTube video by protocolsio

youtu.be

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Stephen Heard @stephenbheard.bsky.social · Jun 3

For scientific writers: Three things your Methods section shouldn't be - but three things Methods sections often end up being. You can avoid these mistakes! scientistseessquirre...

What your Methods section isn’t

If you’re a scientist like me, you read a lot of papers – and a lot of submitted manuscripts, drafts, and other larval stages of papers.* This is extremely helpful when it’s time to write your own …

scientistseessquirrel.wordpress.com

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UCL Office for Open Science and Scholarship @uclopenscience.bsky.social · Jun 2

A fantastic start to #OSFest25 this morning, looking at publishing methods and protocols with @protocolsio.bsky.social. With three different sessions tomorrow and more over the week ahead, it’s not too late to have a look at the festival schedule and register your place: buff.ly/jZsSd6U

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Aligning Science Across Parkinson's @asapresearch.parkinsonsroadmap.org · May 30

This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠

🔗 Check them out: www.protocols.io/view/behavio...

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