… while for larger widths, cells lost the ability to migrate down the gradient due to smoother polarity re-orientation compared to the effective 1D dynamics observed at narrower widths

16/20

As predicted, we found that for intermediate widths, complex trajectories such as circles emerged…

15/20

How can cells migrate down the gradient 🤔?

To figure it out we measure actin retrograde flow, lamellipodia edge extension and polymerization velocity of cells migrating up and down the gradient

10/20

Cells continued to migrate down the gradient!

8/20

But what happened when cells reached the fibronectin peak?

7/20