Some fiery prefrontal cortical neurons projecting to the locus coeruleus that are labeled in the mouse brain

#FluorescenceFriday 🧠🔬🔥

Image credit to @rehg-j.bsky.social and the @lieberinstitute.bsky.social Circuits Team

Glad to have contributed to this cool project!

spaTransfer: transfer learning for single- #cell and spatial #transcriptomics data using non-negative matrix factorization
www.biorxiv.org/content/10.6...

So happy to share this preprint and so proud of @rehg-j.bsky.social and his hard work and determination. Here we showed circuit-specific effect after activation of mPFC-LC projectors to sustained attention during rCPT.

Huge congratulations and kudos to RA @rehg-j.bsky.social @jorge-miranda.bsky.social who led this study that is a continuation of ongoing collaboration between the Circuits group in our division with the @lieberinstitute.bsky.social Behavioral Pharmacology group led by Greg Carr! 🧠🧪

Finally on Bluesky and I’m really excited to share this latest preprint with @jorge-miranda.bsky.social in which we functionally examined the role of a medial prefrontal cortex (mPFC)- Locus Coeruleus (LC) circuit in regulating sustained attention.

1/7

www.biorxiv.org/content/10.6...

For #FluorescenceFriday - cortical neurons projecting to the locus coeruleus 🔵 in the mouse brain 🔬🧠🧪👩‍🔬

Multiple times this week in one of our conference rooms…

HR/leadership peep: That’s weird, who’s trapping who?

Me (visibly excited): Oh!!! That’s probably us!! It’s cells, like some CELLS get trapped, or we trap all the cells!

Blank stares

Me: Like with viruses, in the brain!

Raised eyebrows

Well, for this experiment we definitely TRAPPED some!

Fresh data off the scope from Hopkins Neuro grad student Suhaas Adirajiu for a 2nd #FluorescentFriday from me :)

In honor of some really exciting new data manipulating this cell population - image for #FluorescenceFriday shows activated neurons in the mouse amygdala that project to the lateral septum. Image credit to @rphillips3.bsky.social 🔬🧠😍

The 20X zoom might be even prettier 🤩

Image credits to the Circuits team in the @lieberinstitute.bsky.social Translational Neuroscience division

#FluorescenceFriday

For #FluorescenceFriday - projection neurons from the mouse prefrontal cortex to the locus coeruleus 🔴 with Fos labeling activated cells 🟢. Image credit to the Circuits team in the Translational Neuroscience Division @lieberinstitute.bsky.social 🧠🔬🧪

Me lurking in people's folders on the server for #FluorescenceFriday. Image is so fresh off the scope that I don't even know what it is so I can't even tell you what the channels are - but, somebody (RA Jason Rehg) is doing something in the VTA and whatever it is, it looks super cool 🤩🧠🔬🧪

One more #FluorescenceFriday to give some ❤️ to the mouse 🧠 - I really like the orientation of this slice - locus coeruleus is labeled here with tyrosine hydroxylase in PINK with some virally-labeled axons traversing through in ORANGE 🔬🧪

Image credit to RA Daniel Olivares on our Circuits team!

For #FluorescenceFriday - experiment was apparently a stereotaxic injection "miss", but resulted in a lovely image nevertheless. Credit to @rphillips3.bsky.social and JHU undergrad Natalia Gonzalez-Perez 🧠🔬🧪