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Reto Fiolka

@retof.bsky.social

2.8K followers 1K following 630 posts

Optical imaging, interested in STEM topics. First generation graduate from ETH Zürich Associate Professor at UT Southwestern Medical Center

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Reto Fiolka @retof.bsky.social · 22h

Always keep PSA Flight 182 in mind😞:

en.m.wikipedia.org/wiki/Pacific...

Pacific Southwest Airlines Flight 182 - Wikipedia

en.m.wikipedia.org

Reto Fiolka @retof.bsky.social · 23h

Retro Pacific Southwest Airlines livery on an AA Airbus 320.

Reto Fiolka @retof.bsky.social · 1d

Yes, can be built upright.

Reto Fiolka @retof.bsky.social · 1d

3D tracking of mitochondria DNA (green dots) in a HeLa cell.

Reto Fiolka @retof.bsky.social · 1d

I grew up in Unterseen, not far away from Wengen. Went there for many hikes and skiing in winter. A truly magnificent place!

Thanks for sharing the pictures!

Reto Fiolka @retof.bsky.social · 2d

Volumetric imaging of 384 wells in a contact free format using an air lens.

Importantly, the 3D imaging remains diffraction limited and distortion-free, despite the large refractive index mismatch from air to water.

Reto Fiolka @retof.bsky.social · 2d

Segmented nuclei (gray) and nucleoli (color) in an expanded liver slice:

Reto Fiolka @retof.bsky.social · 2d

Movies:

Microvili formation and dynamics in T-cells.

Reto Fiolka @retof.bsky.social · 2d

Lastly, the zoom lens allows to adapt miOPM to samples with refractive indices (R.I.) different from water, such as cleared tissues.
Below is a comparison of doing CLARITY cleared imaging, with and without adapting the system to the different R.I.

Reto Fiolka @retof.bsky.social · 2d

We can even use an air objective and still perform distortion free and diffraction limited imaging in watery samples.
This can be leveraged to perform contact-free multi-well imaging.

Reto Fiolka @retof.bsky.social · 2d

The same system equipped with a water immersion objective can image larger samples, like an expanded liver slice, organoids or neurons on an EM grid.

Reto Fiolka @retof.bsky.social · 2d

miOPM can use a high numerical oil immersion objective, and perform sensitive live-cell imaging, which we have leveraged to look at T-cell dynamics and track mitochondria DNA in 3D.

Reto Fiolka @retof.bsky.social · 2d

@amsikking.bsky.social invented AIRR; it is an extension of remote focusing theory (Botcherby) in the presence of a refractive index mismatch. In practical terms, one can image distortion-free into a medium different from the immersion of the objective. This gives miOPM its objective flexibility.

Reto Fiolka @retof.bsky.social · 2d

Unlike prior light-sheet systems, miOPM can use a variety of objectives, can be adapted to a wide range of refractive indices and is compatible with standard sample mounting. This comes from two innovations; the any immersion remote refocusing concept, and a zoom lens to adapt the system.

Reto Fiolka @retof.bsky.social · 2d

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

Reposted by Reto Fiolka

eurobioimaging.bsky.social @eurobioimaging.bsky.social · 7d

Meet Andres Bodén! In an interview with @virsicas.bsky.social, he explains how managing the Microscopy Innovation Centre at King's allows him to balance tech development with user projects — to shape the future of microscopy & broaden its impact. 🔬
More⤵️
www.eurobioimaging.eu/news/meet-an...

Reto Fiolka @retof.bsky.social · 6d

Ahh, North Texas is just beautiful in Fall.

Soaking in the views from the Lewisville Landfill.

Reto Fiolka @retof.bsky.social · 7d

Best of luck!

Reto Fiolka @retof.bsky.social · 8d

12 years at UT Southwestern Medical Center, and I still occasionally get lost on its campus.

This time I ended up at the unintentional Halloween portal.

Reposted by Reto Fiolka

(GIRISH)MALI LAB @dyneinassembly.bsky.social · 9d

Happy to share the inaugural paper from the lab. We describe a molecular mechanism for the activation of outer dynein arm motors that power the vital motion of cilia.

Open access link below:
www.nature.com/articles/s41...

Here's a cool animated summary

Reto Fiolka @retof.bsky.social · 9d

Famous writer Roald Dahl on the death of his eldest daughter Olivia.

Somehow this breaks me every time I read it.

Reto Fiolka @retof.bsky.social · 13d

The more I think about it, the more I agree.

So many misunderstandings (malentendus) with it…

Reto Fiolka @retof.bsky.social · 13d

My PhD adviser had this line:

The Optical Etendue is for many “un maletendue”!

😅

Reto Fiolka @retof.bsky.social · 13d

1 MHz line rate is very fast😳!

Time to rename optical invariant to optical flexible😀.

Reto Fiolka @retof.bsky.social · 14d

Awesome news!

www.bbc.com/news/article...

Huntington's disease successfully treated for first time

One of the most devastating diseases finally has a treatment that can slow its progression and transform lives, tearful doctors tell BBC.