Overall we found more than 300 previously unreported RNA isoforms from 31 genes in brain. Some were highly abundant or even the dominant isoform, and we could show translation of novel RNAs into novel proteoforms, including new isoforms of the depression risk gene ITIH4 (see image).

@lh3lh3.bsky.social's latest assembler has really pushed using "routine" ONT libraries (N50s 30KB ish) to get to near T2T assemblies, leveraging read length for (diploid) error correction. Heng continues to be the fountain of excellent sequence bioinformatics tools and it impressive to see.