A laser flash melting procedure, followed by rapid revitrification, provides a simple approach to help mitigate the preferred orientation problem that plagues cryo-EM analysis.

www.nature.com/articles/s41...

Interested in our recent norovirus NS3 work? 👇

Join us to dig deeper!

I'm recruiting a PhD candidate to investigate NS3's role in viral replication and assembly using #cryoEM, #cryoET, and other complementary techniques.

🔗 tinyurl.com/4f3t3aae

Please share! 🙏

Powassan virus is a scary emerging tick-borne virus that causes neurological disease. It is great to see that my @ciddpsu.bsky.social colleagues are on top of it.

www.psu.edu/news/researc...

Huge thanks to my co-authors, the Jose Lab at PSU, Dana Mitzel at USDA, and my advisor @suhafenstein.bsky.social , whose support and guidance made this possible.
Couldn’t have asked for a better team to start this journey.
Excited for what’s next. 💥 (5/5)

This is the first-ever high-resolution structure of the Powassan virus.
It’s basic science, but it opens the door to safer virus research, new vaccines, and antiviral strategies.
Proud to call this the first chapter of my PhD. (4/5)

#ScienceAdvances #CryoEM #Virology #Vaccine #Flavivirus #Powassan

We found three lipid-binding pockets.
Mutating a single tryptophan (W467) in one of them killed the virus.
That residue is conserved across many flaviviruses—Zika, dengue, TBEV...
🚨 A potential universal drug target. (3/5)

We made a chimera: Powassan surface proteins on a yellow fever vaccine backbone.

Then froze it & solved the structure at 2.8 Å via #cryoEM.
Full resolution of E-M proteins, transmembrane helices, and glycosylation. (2/5)

New in @science.org
We built a safe, atomic-resolution version of the Powassan virus—a deadly tick-borne #flavivirus with no vaccine.
✅ First structure ever
✅ Safe to handle (BSL-2)
✅ Platform for vaccines & antivirals

Let me show you 🧵 (1/5)

Link: www.science.org/doi/10.1126/...