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danielhurdiss.bsky.social
Daniel Hurdiss
@danielhurdiss.bsky.social
2.8K followers 1.4K following 820 posts
Assistant Professor at Utrecht University 🇳🇱 We use #cryoEM and molecular #virology techniques to study the infection mechanisms of +ssRNA viruses. https://www.uu.nl/medewerkers/DLHurdiss Views are my own.
www.uu.nl
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danielhurdiss.bsky.social
Daniel Hurdiss @danielhurdiss.bsky.social · Jun 18
Latest preprint from the lab, many years in the making!

By combining #cryoEM with #AlphaFold3 modelling, we propose that norovirus NS3 forms a transmembrane RNA translocase.

This could have big implications for our understanding of viral replication & assembly (🧵)

www.biorxiv.org/content/10.1...
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Reposted by Daniel Hurdiss
pilhoferlab.bsky.social
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · 6h
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social[email protected] trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
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Reposted by Daniel Hurdiss
denkenny.bsky.social
Kenneth Ehses @denkenny.bsky.social · 11h
✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv

Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo

🔗 www.biorxiv.org/content/10.1...
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Reposted by Daniel Hurdiss
cryocloudio.bsky.social
CryoCloud @cryocloudio.bsky.social · 1d
CryoCloud is heading to Women in Electron Microscopy #WeM2025 this week!

We’re proud to sponsor this inaugural event supporting women in electron microscopy.

Our own @ievadr.bsky.social will speak on cloud-native automation to enable high-throughput #cryoEM for drug discovery.

See you there!
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Reposted by Daniel Hurdiss
thijskuiken.bsky.social
Thijs Kuiken @thijskuiken.bsky.social · 1d
Highly pathogenic avian influenza (HPAI) virus detected in a commercial poultry farm (71,000 broiler breeders) in the Netherlands (Gasselternijveenschemond, province Drenthe). All of them will be killed to try to stop the virus from spreading.
www.rijksoverheid.nl/actueel/nieu...
Vogelgriep vastgesteld in Gasselternijveenschemond
In Gasselternijveenschemond (gemeente Aa en Hunze, provincie Drenthe) is vogelgriep vastgesteld op een bedrijf met vleeskuikenouderdieren. Om verspreiding van het virus te voorkomen, worden de circa 7...
www.rijksoverheid.nl
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Reposted by Daniel Hurdiss
daumlab.bsky.social
The Daum Lab @daumlab.bsky.social · 2d
Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail
This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.
www.science.org
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Reposted by Daniel Hurdiss
rcastellsg.bsky.social
Roger Castells @rcastellsg.bsky.social · 3d
We’re looking for an enthusiastic postdoc to join our team. 😊

The project focuses on protein design and cryo-EM applied to cancer research. 🔬🧪

The position is fully funded for 3 years.

Please share 🙏

Job description and application details (deadline: October 11th): www.cnio.es/en/empleo/po...
Postdoctoral Research Assistant for the Biomolecular Design and Structural Nanomedicine Group
DescriptionWe are looking for a Postdoctoral Researcher to join Roger Castells-Graells’ lab at the Spanish National Cancer Research Centre (CNIO). The successful candidate will work on innovative biom...
www.cnio.es
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Reposted by Daniel Hurdiss
harvardcellbio.bsky.social
Harvard Med Cell Biology @harvardcellbio.bsky.social · 6d
Historically, viruses were thought to primarily use host cell's translational machinery. New work from @harvardcellbio.bsky.social faculty Amy Lee reveals that a giant DNA virus encodes its own IF4F initiation complex, suggesting an unexpected evolutionary innovation. www.biorxiv.org/content/10.1...
Giant DNA viruses encode a hallmark translation initiation complex of eukaryotic life
In contrast to living organisms, viruses were long thought to lack protein synthesis machinery and instead depend on host factors to translate viral transcripts. Here, we discover that giant DNA virus...
www.biorxiv.org
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 4d
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail pubmed.ncbi.nlm.nih.gov/41042861/ #cryoem
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Reposted by Daniel Hurdiss
joostsnijder.bsky.social
Joost Snijder @joostsnijder.bsky.social · 7d
We’re hosting the MS+M conference in Utrecht today, lots of great talks ahead at the intersection of mass spec and microscopy. Here’s @hecklab.bsky.social kicking things off.
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Reposted by Daniel Hurdiss
augustsilale.bsky.social
Augustinas Silale @augustsilale.bsky.social · 6d
Our paper on the Bacteroidota BAM complex is out in @natmicrobiol.nature.com! With @madejmar.bsky.social

We found that BAM in Bacteroides and Porphyromonas gingivalis has a distinct architecture from BAM in Proteobacteria.

doi.org/10.1038/s415...
Structure of a distinct β-barrel assembly machinery complex in the Bacteroidota - Nature Microbiology
Structural and functional characterization of the β-barrel assembly machinery complex in Bacteroidota reveals a distinct, seven-component complex with a large extracellular domain that may enable β-barrel–surface lipoprotein complex assembly.
doi.org
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 7d
Understanding the infection mechanism of {Phi}8 using infection method in Pseudomonas syringae pv. Phaseolicola strain: In-situ CryoET analysis www.biorxiv.org/content/10.1101/2025.09.29.678632v1 #cryoem
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 7d
Disulfide-constrained Fabs overcome target size limitation for high-resolution single particle cryoEM pubmed.ncbi.nlm.nih.gov/41027970/ #cryoem
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 7d
Cardiolipin dynamics promote membrane remodeling by mitochondrial OPA1 pubmed.ncbi.nlm.nih.gov/41027961/ #cryoem
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 7d
CXCR4 mediated recognition of HIV envelope spike and inhibition by CXCL12 pubmed.ncbi.nlm.nih.gov/41027939/ #cryoem
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danielhurdiss.bsky.social
Daniel Hurdiss @danielhurdiss.bsky.social · 11d
Epstein–Barr virus exploits desmocollin 2 as the principal epithelial cell entry receptor | @natmicrobiol.nature.com

sco.lt/6i4kd6
Epstein–Barr Virus Uses Desmocollin 2 to Infect Cells | Virus World
In a groundbreaking advancement in virology, scientists have unveiled the elusive mechanism by which Epstein–Barr virus (EBV), a pervasive human herpesvirus, infects epithelial cells. This discovery c...
sco.lt
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danielhurdiss.bsky.social
Daniel Hurdiss @danielhurdiss.bsky.social · 12d
Rounding off an incredibly busy week with the excellent third installment of the Dutch Structural Biology Meeting! Many thanks to Arjen for the kind invitation to present our lab’s work.
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Reposted by Daniel Hurdiss
olibclarke.bsky.social
Oli Clarke @olibclarke.bsky.social · 13d
Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).

A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 14d
gapTrick - Structural characterisation of protein-protein interactions using AlphaFold pubmed.ncbi.nlm.nih.gov/40985612/ #cryoem
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Reposted by Daniel Hurdiss
cryocloudio.bsky.social
CryoCloud @cryocloudio.bsky.social · 14d
September Map of the Month: Small Data, Big Insights ⚡️

From just 233 movies, CryoCloud delivered a 3.2 Å SARS-CoV-2 spike map in 3.5 h.

Fast and efficient #cryoEM, exactly what vaccine design teams need when screening dozens of antigen variants per day.

📬 DM or [email protected] for more info!
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Reposted by Daniel Hurdiss
shyamalm.bsky.social
Shyamal @shyamalm.bsky.social · 15d
Ever wondered how to position organelles in bacteria? Wonder no more!
Happy to have contributed to this fantastic story from @cellforganized.bsky.social' lab.

The avenues this opens in the synthetic biology space are enormous!

Movie: Carboxysomes positioned in E. coli!

Preprint: shorturl.at/D6xVg
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Reposted by Daniel Hurdiss
computingcaitie.bsky.social
Caitie McCafferty @computingcaitie.bsky.social · 15d
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
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Reposted by Daniel Hurdiss
Thelonevirologist @thelonevirologist.bsky.social · 16d
Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation

journals.plos.org/plospathogen...
Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation
Author summary Human astroviruses (HAstV) are understudied, globally prevalent pathogens capable of causing potentially fatal infections of the central nervous system in children, the elderly, and imm...
journals.plos.org
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 15d
The structure of the Human Adenovirus 7 virus-like particles reveals that pentons and core-genome promote hexon-pIIIa interactions during capsid maturation www.biorxiv.org/content/10.1101/2025.09.22.677823v1 #cryoem
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Reposted by Daniel Hurdiss
Thelonevirologist @thelonevirologist.bsky.social · 15d
Hepatitis C virus NS3/4A protease cleaves SPG20, a key regulator of lipid droplet turnover, to promote lipid droplet formation journals.asm.org/doi/10.1128/...
Hepatitis C virus NS3/4A protease cleaves SPG20, a key regulator of lipid droplet turnover, to promote lipid droplet formation | Journal of Virology
HCV infection often promotes the formation of large LDs in HCV-infected cells. However, the molecular mechanism underlying large LD formation is poorly understood. LD turnover is regulated by SPG20, I...
journals.asm.org
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Reposted by Daniel Hurdiss
cryoempapers.bsky.social
cryoEM papers @cryoempapers.bsky.social · 18d
Rapid elicitation of a new class of neutralizing N332-glycan independent V3-glycan antibodies against HIV-1 in nonhuman primates pubmed.ncbi.nlm.nih.gov/40964314/ #cryoem
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