4/4 This work was a true team effort between @amolf-nl.bsky.social, IHB Basel, and the @Hubrechtinstitute.bsky.social — led by @danielkrueger.bsky.social and Kasper Spoelstra. Huge thanks to all collaborators for making it possible.

3/4 This reveals a safeguard mechanism: epithelial cells constantly test each other’s mechanical strength. Too little (or too much) force destabilizes the barrier, linking extrusion to diseases like tufting enteropathy. Gut renewal is an active, force-regulated process.

2/4 Using live imaging, CRISPR myosin reporters, organoid villus substrates & optogenetics, we found that myosin-driven tissue tension—not compression—controls extrusion. Cells with reduced contractility are preferentially removed.

1/4 New in Science: Gut renewal is not passive “crowd control.” Instead of being pushed out by crowding or dying from apoptosis, cells compete in a mechanical tug-of-war. Weaker cells are eliminated—reframing intestinal homeostasis as a force-regulated process.
www.science.org/doi/10.1126/...

2/4 Using live imaging, CRISPR myosin reporters, organoid villus substrates & optogenetics, we found that myosin-driven tissue tension—not compression—controls extrusion. Cells with reduced contractility are preferentially removed.

Do you want to learn how to make mouse thymus organoids?

Our lab recently published a STAR Protocol on generating thymic epithelial organoids from adult mouse thymus tissue.

Read it here: doi.org/10.1016/j.xp...

#Thymus #Organoids #STARProtocol #TEC

Our PhD student @samwillemsen.bsky.social presented our work on thymus organoids at this year’s Global Thymus Network meeting, ThymUS 2025.

#ThymUS2025 #Organoids #Thymus

The Clevers lab is in Spain! Our postdocs Hanxiao, @sarinashabso.bsky.social, @amandaanderssonr.bsky.social and a PhD student @katarinabalazova.bsky.social are taking part in an @embo.org Workshop on Liver and Pancreas in Metabolic Diseases in Girona 🌞 #embo #liver #pancreas #girona

IFN-responsive intestinal BEST4/CA7+ cells are targets of bacterial diarrheal toxins @cp-cellstemcell.bsky.social @thecleverslab.bsky.social
www.cell.com/cell-stem-ce...

Organoid drug profiling identifies methotrexate as a therapy for SCCOHT, a rare pediatric cancer
www.science.org/doi/10.1126/...
@thecleverslab.bsky.social

Check out this new article highlighting our postdoc @amandaanderssonr.bsky.social ’s work on a novel pancreatic organoid model that includes all three key cell types, offering new insights into pancreatic development: www.the-scientist.com/a-novel-thre...

📢New human fetal pancreatic organoid model generates all three key cell types of the pancreas. New paper in @cellpress.bsky.social by @amandaanderssonr.bsky.social , @hansclevers.bsky.social and collaborators. See the article here: www.sciencedirect.com/science/arti...