winterhalterlab.bsky.social
Charles Winterhalter
@winterhalterlab.bsky.social
90 followers 130 following 4 posts
Wellcome Early-Career Fellow investigating novel DNA damage repair mechanisms in bacteria at Newcastle university (UK)
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winterhalterlab.bsky.social
Charles Winterhalter @winterhalterlab.bsky.social · Mar 11
Excited to share with you the story that enabled me to start my own lab: what happens when the bacterial DNA replication machinery encounters single-strand discontinuities on template DNA? What is required for break repair and replication restart?
www.biorxiv.org/content/10.1...
Rescuing bacterial genome replication: essential functions to repair a double-strand break and restart DNA synthesis
Many antibiotics indirectly generate reactive oxygen species (ROS) that can damage bacterial genomes. Oxidised nucleobases become genotoxic when they are targeted for repair through excision, generati...
www.biorxiv.org
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Reposted by Charles Winterhalter
chillzaa.bsky.social
Chris H. Hill @chillzaa.bsky.social · Mar 21
New cryo-EM/ET story - a brilliant collaboration with Sergey Melnikov's lab in Newcastle @sergeymelnikov.bsky.social and Stefan Pfeffer's lab in Heidelberg @pfeffercryolab.bsky.social. Great work in particular by Karla Helena-Bueno and @sophiekopetschke.bsky.social

www.nature.com/articles/s41...
Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells - Nature Communications
Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that distinct ribosomes cooperate rath...
www.nature.com
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winterhalterlab.bsky.social
Charles Winterhalter @winterhalterlab.bsky.social · Mar 20
Still time to apply and join my team on a fully funded PhD project looking at dynamics of the bacterial DNA damage response in live cells using genomics.
cbcb-newcastle.bsky.social
CBCB Newcastle @cbcb-newcastle.bsky.social · Mar 20
Engineering anti-CRISPR/Cas systems to control the DNA damage response in live bacterial cells. Supervised by @winterhalterlab.bsky.social, @acdarby.bsky.social, and Katarzyna Mickiewicz.

www.findaphd.com/phds/project...
Engineering anti-CRISPR/Cas systems to control the DNA damage response in live bacterial cells at Newcastle University on FindAPhD.com
PhD Project - Engineering anti-CRISPR/Cas systems to control the DNA damage response in live bacterial cells at Newcastle University, listed on FindAPhD.com
www.findaphd.com
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Reposted by Charles Winterhalter
gruberlab.bsky.social
Stephan Gruber @gruberlab.bsky.social · Mar 16
This was a fantastic collaboration with our neighbors @mblokesch.bsky.social & David Adams and Mark Szczelkun and @steven-shaw.bsky.social in Bristol. Special thanks to the DCI for invaluable support with cryo-EM of a rather difficult target! @drhonsworth.bsky.social
www.biorxiv.org/content/10.1...
Structure and Activation Mechanism of a Lamassu Phage Defence System
Lamassu is a diverse family of defence systems that protect bacteria, including pandemic strains of Vibrio cholerae, against phage infection. They target essential cellular processes, aborting infecti...
www.biorxiv.org
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winterhalterlab.bsky.social
Charles Winterhalter @winterhalterlab.bsky.social · Mar 11
With a very big thank you to Heath from @heathmurraylab.bsky.social who has been an incredible mentor over the years and offered me amazing support throughout my journey towards research independence
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winterhalterlab.bsky.social
Charles Winterhalter @winterhalterlab.bsky.social · Mar 11
Beautiful work from the Dewar lab and colleagues diving into replication fork rescue in vertebrates. Together, our papers highlight critical similarities/differences in DNA damage repair processes occurring in prokaryotes vs eukaryotes when the replisome encounters a single-strand discontinuity.
jamesmdewar.bsky.social
James Dewar @jamesmdewar.bsky.social · Mar 10
🧬⛓️‍ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Resolution of collapsed forks is separate from completion of DNA synthesis
Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...
www.biorxiv.org
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winterhalterlab.bsky.social
Charles Winterhalter @winterhalterlab.bsky.social · Mar 11
Excited to share with you the story that enabled me to start my own lab: what happens when the bacterial DNA replication machinery encounters single-strand discontinuities on template DNA? What is required for break repair and replication restart?
www.biorxiv.org/content/10.1...
Rescuing bacterial genome replication: essential functions to repair a double-strand break and restart DNA synthesis
Many antibiotics indirectly generate reactive oxygen species (ROS) that can damage bacterial genomes. Oxidised nucleobases become genotoxic when they are targeted for repair through excision, generati...
www.biorxiv.org
1 14 21