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xipeng1.bsky.social @xipeng1.bsky.social · 4d

Very proud to be the first PKU Talks interviewed professor!😀😀😀

Reposted

Uri Manor @manorlaboratory.bsky.social · Aug 19

Absolutely beautiful paper with some awesome imaging of our AC-ER probe for monitoring ER-associated actin (PMID: 32778832) 🤯

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xipeng1.bsky.social @xipeng1.bsky.social · 4d

Very proud to be the first PKU Talks interviewed professor!😀😀😀Come and take a look!

Peking University @pku1898.bsky.social · 11d

🔬Ever wondered what a microscopy pioneer would pack for a one-way Mars trip?

Dive into our debut episode of “Walk This Way: PKU Talks” with Prof. Xi Peng—strolling through his lab, sharing SIM breakthroughs, his favorite movie, and more...

📺: www.youtube.com/watch?v=tq-q...

@xipeng1.bsky.social

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xipeng1.bsky.social @xipeng1.bsky.social · Aug 15

Nat. Photonics🔬 Meet 3I-SIM!
Instead of stripes, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 frames, 13 h neuronal cell movies, ER–lipid-droplet kiss-n-run and millisecond tango. All hard&software open.
doi.org/10.1038/s415... @pku1898.bsky.social

Triangle-beam interference structured illumination microscopy - Nature Photonics

Triangle-beam interference structured illumination microscopy leverages radially polarized beams to generate two-dimensional lattice illumination patterns. The technique enables a temporal resolution ...

doi.org

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xipeng1.bsky.social @xipeng1.bsky.social · Aug 4

C²SD-ISM combines physical defocus rejection (spinning disk) with adaptive algorithm (DPA-PR), breaking depth limits in tissue super-resolution (↑180 μm), enhances fidelity (92% linear correlation), and supports high-throughput imaging. doi.org/10.1038/s413... Github: github.com/Chauncey-Leu....

High-fidelity tissue super-resolution imaging achieved with confocal2 spinning-disk image scanning microscopy - Light: Science & Applications

C²SD-ISM integrates an SD and a DMD to form a dual confocal configuration, enabling deep-tissue super-resolution imaging with DPA-PR reconstruction.

doi.org

xipeng1.bsky.social @xipeng1.bsky.social · Jun 19

Ultra-stable, ultra bright, congratulations!

Reposted

piatkevich.bsky.social @piatkevich.bsky.social · Jun 18

mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

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xipeng1.bsky.social @xipeng1.bsky.social · Jun 19

Congratulations to Suyi Zhong, Wei Ren, Ziyi Yang, and Hongyu Wang!

xipeng1.bsky.social @xipeng1.bsky.social · May 31

If you have a dual channel, it can certainly be implemented on either one, or both.😁

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xipeng1.bsky.social @xipeng1.bsky.social · May 31

Oh no, it does not need any hardware modification. It is like any Fiji plugin - it removes the fluorescence background through algorithm.😁

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xipeng1.bsky.social @xipeng1.bsky.social · May 30

Congratulations Stephan!🎉🎉🎉

xipeng1.bsky.social @xipeng1.bsky.social · May 30

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social

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Reposted

Zhixing Chen @zhixingchen2.bsky.social · May 20

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com

A palette of bridged bicycle-strengthened fluorophores - Nature Methods

A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...

www.nature.com

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xipeng1.bsky.social @xipeng1.bsky.social · May 30

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social

xipeng1.bsky.social @xipeng1.bsky.social · May 30

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social

xipeng1.bsky.social @xipeng1.bsky.social · May 30

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social

Zhixing Chen @zhixingchen2.bsky.social · May 20

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com

A palette of bridged bicycle-strengthened fluorophores - Nature Methods

A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...

www.nature.com

xipeng1.bsky.social @xipeng1.bsky.social · May 30

Thrilled to introduce our Cover review: High-spatiotemporal-resolution structured illumination microscopy: principles, instrumentation, and applications dx.doi.org/10.3788/PI.2...

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xipeng1.bsky.social @xipeng1.bsky.social · May 29

Beautiful mitochondria!👍👍👍

xipeng1.bsky.social @xipeng1.bsky.social · May 29

Hi Rita, nice to see you at Bluesky! 😁😁😁

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xipeng1.bsky.social @xipeng1.bsky.social · May 29

Unlock the secrets of cellular dynamics with high-spatiotemporal-resolution structured illumination microscopy! Our latest review explores the principles, instrumentation, and applications of SIM&ISM in live-cell imaging. #Microscopy #SuperResolution #CellBiology dx.doi.org/10.3788/PI.2...

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Reposted

Peking University @pku1898.bsky.social · May 27

Seeing Deeper, Clearer than Ever!🔬Prof. Xi Peng’s team from #PKU College of Future Technology introduces Dark Sectioning in Nature Methods—a breakthrough for fluorescence microscopy!

A peek into the future of imaging:
www.nature.com/articles/s41...

#PKUResearch #AcademicSky @natmethods.nature.com

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xipeng1.bsky.social @xipeng1.bsky.social · May 18

Dark matter, Dark channel matters! 😀
Link to our paper and commentary: www.nature.com/articles/s41... www.nature.com/articles/s41...

Dark-based optical sectioning assists background removal in fluorescence microscopy - Nature Methods

The Dark sectioning algorithm removes the background and provides single-frame optical sectioning in fluorescence microscopy. It offers improved quantitative analysis and deep-tissue segmentation accu...

www.nature.com

Reposted

Nature Methods @natmethods.nature.com · May 13

For a quick summary of the paper, read the Research Briefing here:
www.nature.com/articles/s41...

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xipeng1.bsky.social @xipeng1.bsky.social · May 16

Thank you! 😁😁😁

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Reposted

Emmanuel Soubies @esoubies.bsky.social · May 15

Nice work! We integrated this dark-sectioning method into our FlexSIM pipeline (github.com/esoubies/Fle...) as an additional option for handling out-of-focus signal. It works remarkably well!

Nature Methods @natmethods.nature.com · May 12

A Dark sectioning algorithm removes background and provides single-frame optical sectioning in fluorescence microscopy, facilitating improved deep-tissue segmentation accuracy.

www.nature.com/articles/s41...

Dark-based optical sectioning assists background removal in fluorescence microscopy - Nature Methods

The Dark sectioning algorithm removes the background and provides single-frame optical sectioning in fluorescence microscopy. It offers improved quantitative analysis and deep-tissue segmentation accu...

www.nature.com

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