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Reposted by Zenlabpasteur.bsky.social

Institut Pasteur Education @pasteuredu.bsky.social · 19d

⌛1 month left to apply – 2025 PPU call for candidates

We invite you to discover 4 Develpmental & Stem Cell Biology projects and 3 Immunology projects👇

🕰️Application deadline: October 20, 2025
📍Program starts: October 2026

Discover all the projects (50+) & apply: www.pasteur.fr/en/education...

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Gaëlle Letort @gaellel.bsky.social · Sep 4

We (with @nicolasdray.bsky.social @zenlabpasteur.bsky.social) are happy to release officially FishFeats, our #napari plugin to streamline 3D smFish/RNA quantification! 🎊
Available here: github.com/gletort/Fish... user documentation: gletort.github.io/FishFeats/

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Development @dev-journal.bsky.social · Aug 15

Pathway to Independence – an interview with Joaquín Navajas Acedo.

In this interview, @mads100tist.bsky.social talks about about his research vision, zebrafish and the importance of community:

journals.biologists.com/dev/article/...

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Aug 5

New preprint out! Novel transgenic lines directly read out Notch3 signaling in situ, and reveal how adult neural stem cells coordinate quiescence and stemness by integrating spatiotemporal information from Delta and Jagged ligands. Read more: doi.org/10.1101/2025...

Translocation of the Notch3 Intracellular Domain (green) to the nucleus in adult neural stem cells (top), but retention at the membrane upon gamma-secretase inhibition (bottom).

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Jul 11

Engineer position, 12 months starting Oct. 1st, 2025, to study the role of NOTCH3 in human iPSC-derived neurogenesis models. Solid previous expertise required in cell culture, notably iPSCs, and organoid generation. Send CV, motivation letter and reference letter(s) to [email protected]

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Dec 8

New call for research Group Leaders at Institut Pasteur Paris !

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 24

Just published in Development (@biologists.bsky.social):
doi.org/10.1242/dev....

Non-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cells

Highlighted Article: An inducible Cas3*/Cas7* sensor demonstrates that non-apoptotic caspase events occur in zebrafish neural stem cells. In cooperation with the stress-induced transcription factor At...

doi.org

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Developmental & Stem Cell Biology Pasteur, CNRS UMR3738 @devstempasteur.bsky.social · Nov 20

On Mon 18/11, we held a mini symposium on the novel perspectives in human embryo research @institutpasteur. Lively discussions thanks to our prominent speakers/chair
Thanks to Nicolas Rivron; Alain Chédotal; Cécile Martinat; Ken McElreavey; Bernadette De Bakker and the chair Alfonso Martinez Arias

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

This also draws yet another parallel between physiological regulation stem cell pools and oncogenesis, which has been referred to as a process through which cells become “asocial” and cease to coordinate with their neighbors (18/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Although we were not able to identify what advantage this confers for NSC homeostasis, this non-canonical maintenance of Notch effectors illustrates different scales of quiescence regulation with some cells seemingly being able to listen less to their neighbors (17/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

In vivo experiments confirmed that expression of nr2f1b in zebrafish NSCs is associated with a weaker response to Notch inhibition and functional experiments with morpholinos or electroporations of nr2f1b confirmed its ability to decrease sensitivity to Notch inhibition. (16/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Interestingly NR2F1 had previously been identified as a regulator of Notch signaling in the mouse inner ear (journals.biologists.com/dev/article/...), with downregulation of Notch effectors in NR2F1 knock-out mutants (15/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Using a gradient boosting method derived from SCENIC applied to both control and treated cells, we identified NR2F1/Coup-TF1 in sillico as a putative regulator enabling this resistance to Notch inhibition. (14/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Finally, we also identified that one cluster of cells barely responded to Notch inhibition, maintained expression of Notch effectors and did not upregulate genes associated with NSC activation. (13/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Although there are obvious differences between parenchymal astrocytes in mouse and astrocyte-like RGs in zebrafish, this suggests that comparative studies focusing on these cells might help better understand how to promote brain regeneration. (12/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

The reported properties of the induced activation of these cells indeed shared similarities with that of astrocyte-like NSCs in the zebrafish pallium, such as participation in regeneration or reliance on Notch signaling (11/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Harmonizing the different reports of cell classification led us to consider that the cells that had been described as latent NSCs under different names in different publications were in fact likely the same population of parenchymal astrocytes close to the lateral ventricles. 10/18

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Using the set of genes which we previously identified as allowing for distinction between murine RGL and astrocytes, we reclassified qNSC1 cells as astrocytes, in accordance with scRNAseq and in situ data from A Cebrian-Silla and methylome data from LPM Kremer. (9/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

We found that the distinction between qNSC1 and qNSC2 had not been made in most cases even when the cells belonging to each group were present in the dataset. (8/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

To get a clearer picture we re-analyzed scRNA-seq datasets encompassing the SVZ, generated by the labs of F Doetsch (@doetschlab.bsky.social), A Martin Villalba, J Frisen, S Linnarsson (@slinnarsson.bsky.social), J Biernaskie, N Rajewsky, F Miller, A Alvarez Buylla and O Raineteau. (7/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

A similar phenomenon had been described in mouse astrocytes and putative subventricular zone (SVZ) radial glia-like (RGL) (called qNSC1) which respond to injury. (6/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

In particular we found that one group of NSCs, recently described as being very similar to mouse parenchymal astrocytes, is efficiently recruited by Notch inhibition leading to the appearance of a cell state defined by co-expression of astrocytic and activating NSCs markers. (5/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

With scRNAseq after 24hours of Notch inhibition we found that most cells had responded, but not in the same way. (4/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

We attempted to push NSCs out of quiescence using Notch inhibition to see if previously identified clusters reacted differently to a pro-activation stimulus. (3/18)

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Zenlabpasteur.bsky.social @zenlabpasteur.bsky.social · Nov 17

Drawing from previous characterization of the heterogeneity of adult Neural Stem Cells (qNSCs) in the zebrafish pallium and the prediction that some of that heterogeneity comes from differences in quiescence depth (2/18)

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