#PeripheralArteryDisease
#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia
Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)
#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia
Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)
#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
March 2, 2025 at 7:55 PM
#PeripheralArteryDisease
#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia
Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)
#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia
Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)
#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
Multimodal non-invasive imaging of mouse #LimbIschemia
#LaserSpeckleImaging
#NearInfrared2ZoneImaging
#DuplexUltrasound Flow + Muscle elastography
#OpticalCoherenceTomographyAngiography
+Some human data #T2D
#BioengTranslMed 2025
aiche.onlinelibrary.wiley.com/doi/10.1002/...
#LaserSpeckleImaging
#NearInfrared2ZoneImaging
#DuplexUltrasound Flow + Muscle elastography
#OpticalCoherenceTomographyAngiography
+Some human data #T2D
#BioengTranslMed 2025
aiche.onlinelibrary.wiley.com/doi/10.1002/...
November 8, 2025 at 11:15 PM
Multimodal non-invasive imaging of mouse #LimbIschemia
#LaserSpeckleImaging
#NearInfrared2ZoneImaging
#DuplexUltrasound Flow + Muscle elastography
#OpticalCoherenceTomographyAngiography
+Some human data #T2D
#BioengTranslMed 2025
aiche.onlinelibrary.wiley.com/doi/10.1002/...
#LaserSpeckleImaging
#NearInfrared2ZoneImaging
#DuplexUltrasound Flow + Muscle elastography
#OpticalCoherenceTomographyAngiography
+Some human data #T2D
#BioengTranslMed 2025
aiche.onlinelibrary.wiley.com/doi/10.1002/...
Spatial Atlas of muscular & vascular regeneration in 🐭#LimbIschemia
From our Pickering lab😁
3 positions covering 22 hindlimb muscles
Tight #Angiogeneis-#Myogenesis coupling leaves little border zone in ischemic-regenerating (day 10)🐭muscle
#ATVB 2020
www.ahajournals.org/doi/full/10....
From our Pickering lab😁
3 positions covering 22 hindlimb muscles
Tight #Angiogeneis-#Myogenesis coupling leaves little border zone in ischemic-regenerating (day 10)🐭muscle
#ATVB 2020
www.ahajournals.org/doi/full/10....
October 18, 2024 at 7:11 PM
Spatial Atlas of muscular & vascular regeneration in 🐭#LimbIschemia
From our Pickering lab😁
3 positions covering 22 hindlimb muscles
Tight #Angiogeneis-#Myogenesis coupling leaves little border zone in ischemic-regenerating (day 10)🐭muscle
#ATVB 2020
www.ahajournals.org/doi/full/10....
From our Pickering lab😁
3 positions covering 22 hindlimb muscles
Tight #Angiogeneis-#Myogenesis coupling leaves little border zone in ischemic-regenerating (day 10)🐭muscle
#ATVB 2020
www.ahajournals.org/doi/full/10....
Perivascular Podoplanin+ fibroblasts/mesenchymal cells secrete CSF-1, potentially synergizing with endothelial-derived DLL1, to promote macrophage differentiation/polarization & expansion in 🐭#LimbIschemia
Dr. Florian Limbourg lab Front Immunol 2023
www.frontiersin.org/articles/10....
Dr. Florian Limbourg lab Front Immunol 2023
www.frontiersin.org/articles/10....
September 30, 2023 at 11:53 AM
Perivascular Podoplanin+ fibroblasts/mesenchymal cells secrete CSF-1, potentially synergizing with endothelial-derived DLL1, to promote macrophage differentiation/polarization & expansion in 🐭#LimbIschemia
Dr. Florian Limbourg lab Front Immunol 2023
www.frontiersin.org/articles/10....
Dr. Florian Limbourg lab Front Immunol 2023
www.frontiersin.org/articles/10....
Bispecific antibody for #LimbIschemia
A dual-targeting antibody T11-RF12 for activating Tie2 & LGR5-FZD10 Wnt signaling in #EndothelialCells
Intramuscular injection (Gracilis) Balb/c🐭
⏫limb reperfusion
Rescue paw necrosis
⏫Angiogenesis Arteriogenesis
mAbs 2024
www.tandfonline.com/doi/10.1080/...
A dual-targeting antibody T11-RF12 for activating Tie2 & LGR5-FZD10 Wnt signaling in #EndothelialCells
Intramuscular injection (Gracilis) Balb/c🐭
⏫limb reperfusion
Rescue paw necrosis
⏫Angiogenesis Arteriogenesis
mAbs 2024
www.tandfonline.com/doi/10.1080/...
December 3, 2024 at 12:30 PM
Bispecific antibody for #LimbIschemia
A dual-targeting antibody T11-RF12 for activating Tie2 & LGR5-FZD10 Wnt signaling in #EndothelialCells
Intramuscular injection (Gracilis) Balb/c🐭
⏫limb reperfusion
Rescue paw necrosis
⏫Angiogenesis Arteriogenesis
mAbs 2024
www.tandfonline.com/doi/10.1080/...
A dual-targeting antibody T11-RF12 for activating Tie2 & LGR5-FZD10 Wnt signaling in #EndothelialCells
Intramuscular injection (Gracilis) Balb/c🐭
⏫limb reperfusion
Rescue paw necrosis
⏫Angiogenesis Arteriogenesis
mAbs 2024
www.tandfonline.com/doi/10.1080/...
MedSky🧪#IDSky CardioSky🫀 #CaseReport #COVID19 leads to a #hypercoagulable state that predisposes patients to #thromboembolism. Thrombotic events occur in the arterial circulation in 1-5% of cases & may cause #stroke, #acutecoronarysyndrome or #Limbischemia .
December 28, 2023 at 12:53 PM
MedSky🧪#IDSky CardioSky🫀 #CaseReport #COVID19 leads to a #hypercoagulable state that predisposes patients to #thromboembolism. Thrombotic events occur in the arterial circulation in 1-5% of cases & may cause #stroke, #acutecoronarysyndrome or #Limbischemia .
🐷bone marrow #MesenchymalStemCell encapsulated in Alginate #Hydrogel as cell therapy in 🐷model of #LimbIschemia Iliac arterial excision
Some MSCs survive up to 4 wk in vivo
Limb use - force-time impulses✅ trotting pattern✅
Hyperemic perfusion✅
#JAHA 2024
www.ahajournals.org/doi/10.1161/...
Some MSCs survive up to 4 wk in vivo
Limb use - force-time impulses✅ trotting pattern✅
Hyperemic perfusion✅
#JAHA 2024
www.ahajournals.org/doi/10.1161/...
April 23, 2024 at 6:56 PM
🐷bone marrow #MesenchymalStemCell encapsulated in Alginate #Hydrogel as cell therapy in 🐷model of #LimbIschemia Iliac arterial excision
Some MSCs survive up to 4 wk in vivo
Limb use - force-time impulses✅ trotting pattern✅
Hyperemic perfusion✅
#JAHA 2024
www.ahajournals.org/doi/10.1161/...
Some MSCs survive up to 4 wk in vivo
Limb use - force-time impulses✅ trotting pattern✅
Hyperemic perfusion✅
#JAHA 2024
www.ahajournals.org/doi/10.1161/...
Activating #HexosamineBiosynthesisPathway to treat #PeripheralArteryDisease
Glucosamine i.p. ⏫Reperfusion #Angiogenesis but not #Arteriogenesis in🐭#LimbIschemia (BALB/c, eNOS-/- C57BL/6)
Distinct pathways from VEGFA
ATF4 as a signaling mediator
#CircAHA 2024
www.ahajournals.org/doi/10.1161/...
Glucosamine i.p. ⏫Reperfusion #Angiogenesis but not #Arteriogenesis in🐭#LimbIschemia (BALB/c, eNOS-/- C57BL/6)
Distinct pathways from VEGFA
ATF4 as a signaling mediator
#CircAHA 2024
www.ahajournals.org/doi/10.1161/...
November 14, 2024 at 1:04 PM
Activating #HexosamineBiosynthesisPathway to treat #PeripheralArteryDisease
Glucosamine i.p. ⏫Reperfusion #Angiogenesis but not #Arteriogenesis in🐭#LimbIschemia (BALB/c, eNOS-/- C57BL/6)
Distinct pathways from VEGFA
ATF4 as a signaling mediator
#CircAHA 2024
www.ahajournals.org/doi/10.1161/...
Glucosamine i.p. ⏫Reperfusion #Angiogenesis but not #Arteriogenesis in🐭#LimbIschemia (BALB/c, eNOS-/- C57BL/6)
Distinct pathways from VEGFA
ATF4 as a signaling mediator
#CircAHA 2024
www.ahajournals.org/doi/10.1161/...
(Mouse/Human) Mitochondria transplantation i.m. improves reperfusion #Angiogenesis of 🐭#LimbIschemia
Macrophage, instead of #EndothelialCell, is the major cell type that internalizes exogenous Mitochondria (sustained by day 3 not 7)
#BioactiveMat 2025
www.sciencedirect.com/science/arti...
Macrophage, instead of #EndothelialCell, is the major cell type that internalizes exogenous Mitochondria (sustained by day 3 not 7)
#BioactiveMat 2025
www.sciencedirect.com/science/arti...
July 22, 2025 at 6:53 PM
(Mouse/Human) Mitochondria transplantation i.m. improves reperfusion #Angiogenesis of 🐭#LimbIschemia
Macrophage, instead of #EndothelialCell, is the major cell type that internalizes exogenous Mitochondria (sustained by day 3 not 7)
#BioactiveMat 2025
www.sciencedirect.com/science/arti...
Macrophage, instead of #EndothelialCell, is the major cell type that internalizes exogenous Mitochondria (sustained by day 3 not 7)
#BioactiveMat 2025
www.sciencedirect.com/science/arti...
#PeripheralArteryDisease #Netrin
Exosome derived from Netrin 1-overexpressing 🐭#AdiposeDerivedStromalCell⏫
Reperfusion #Angiogenesis 🧐 #Arteriogenesis
Exercise capacity
M2 Mφ polarization
in Diabetic db/db🐭#LimbIschemia
#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...
Exosome derived from Netrin 1-overexpressing 🐭#AdiposeDerivedStromalCell⏫
Reperfusion #Angiogenesis 🧐 #Arteriogenesis
Exercise capacity
M2 Mφ polarization
in Diabetic db/db🐭#LimbIschemia
#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...
December 4, 2024 at 1:48 PM
#PeripheralArteryDisease #Netrin
Exosome derived from Netrin 1-overexpressing 🐭#AdiposeDerivedStromalCell⏫
Reperfusion #Angiogenesis 🧐 #Arteriogenesis
Exercise capacity
M2 Mφ polarization
in Diabetic db/db🐭#LimbIschemia
#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...
Exosome derived from Netrin 1-overexpressing 🐭#AdiposeDerivedStromalCell⏫
Reperfusion #Angiogenesis 🧐 #Arteriogenesis
Exercise capacity
M2 Mφ polarization
in Diabetic db/db🐭#LimbIschemia
#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...
#Nanoceria-coated #GrapheneOxide microsheet as multicellular (HUVEC+MSC) delivery system to treat 🐭#LimbIschemia
⏬early (72 h) ROS
⏫early HIF1α (in what cell types?)
⏫#Angiogenesis+#Arteriogenesis in ischemic adductor muscle
#Biomaterials 2023
www.sciencedirect.com/science/arti...
⏬early (72 h) ROS
⏫early HIF1α (in what cell types?)
⏫#Angiogenesis+#Arteriogenesis in ischemic adductor muscle
#Biomaterials 2023
www.sciencedirect.com/science/arti...
March 23, 2024 at 7:06 PM
#Nanoceria-coated #GrapheneOxide microsheet as multicellular (HUVEC+MSC) delivery system to treat 🐭#LimbIschemia
⏬early (72 h) ROS
⏫early HIF1α (in what cell types?)
⏫#Angiogenesis+#Arteriogenesis in ischemic adductor muscle
#Biomaterials 2023
www.sciencedirect.com/science/arti...
⏬early (72 h) ROS
⏫early HIF1α (in what cell types?)
⏫#Angiogenesis+#Arteriogenesis in ischemic adductor muscle
#Biomaterials 2023
www.sciencedirect.com/science/arti...
Bone in an Artery!😍
A beautiful histopathologic study on #MedialCalcification & #IntimalCalcification Lower Limb Arteries
> 2k sections, 77 (femoropopliteal/anterior tibial/posterior tibial/peroneal/) arteries, 36 #LimbIschemia👤
Sho Torii lab 2024 #JACCAdv
www.sciencedirect.com/science/arti...
A beautiful histopathologic study on #MedialCalcification & #IntimalCalcification Lower Limb Arteries
> 2k sections, 77 (femoropopliteal/anterior tibial/posterior tibial/peroneal/) arteries, 36 #LimbIschemia👤
Sho Torii lab 2024 #JACCAdv
www.sciencedirect.com/science/arti...
June 30, 2024 at 7:08 PM
Bone in an Artery!😍
A beautiful histopathologic study on #MedialCalcification & #IntimalCalcification Lower Limb Arteries
> 2k sections, 77 (femoropopliteal/anterior tibial/posterior tibial/peroneal/) arteries, 36 #LimbIschemia👤
Sho Torii lab 2024 #JACCAdv
www.sciencedirect.com/science/arti...
A beautiful histopathologic study on #MedialCalcification & #IntimalCalcification Lower Limb Arteries
> 2k sections, 77 (femoropopliteal/anterior tibial/posterior tibial/peroneal/) arteries, 36 #LimbIschemia👤
Sho Torii lab 2024 #JACCAdv
www.sciencedirect.com/science/arti...
#ChronicLimbThreateningIschemia #LimbIschemia
Competing risk analysis to estimate #Amputation incidence & risk in #LowerExtremityPeripheralArteryDisease
7273 case + 5095 ctrl
2 yr f/up
journals.sagepub.com/doi/10.1177/...
Competing risk analysis to estimate #Amputation incidence & risk in #LowerExtremityPeripheralArteryDisease
7273 case + 5095 ctrl
2 yr f/up
journals.sagepub.com/doi/10.1177/...
September 21, 2024 at 8:04 PM
#ChronicLimbThreateningIschemia #LimbIschemia
Competing risk analysis to estimate #Amputation incidence & risk in #LowerExtremityPeripheralArteryDisease
7273 case + 5095 ctrl
2 yr f/up
journals.sagepub.com/doi/10.1177/...
Competing risk analysis to estimate #Amputation incidence & risk in #LowerExtremityPeripheralArteryDisease
7273 case + 5095 ctrl
2 yr f/up
journals.sagepub.com/doi/10.1177/...
Mouse #LimbIschemia
Contrast-enhanced ultrasound (whole calf) vs Laser Doppler (primarily skin) imaging of limb reperfusion post-ischemia
C57BL/6J🐭♂️ 10~12 wk
B/w 2- & 4-wk post-ischemia
Laser doppler ⬆️
Ultrasound microbubble ⬇️
Ultrasound Med Biol 2024
www.sciencedirect.com/science/arti...
Contrast-enhanced ultrasound (whole calf) vs Laser Doppler (primarily skin) imaging of limb reperfusion post-ischemia
C57BL/6J🐭♂️ 10~12 wk
B/w 2- & 4-wk post-ischemia
Laser doppler ⬆️
Ultrasound microbubble ⬇️
Ultrasound Med Biol 2024
www.sciencedirect.com/science/arti...
October 24, 2024 at 7:02 PM
Mouse #LimbIschemia
Contrast-enhanced ultrasound (whole calf) vs Laser Doppler (primarily skin) imaging of limb reperfusion post-ischemia
C57BL/6J🐭♂️ 10~12 wk
B/w 2- & 4-wk post-ischemia
Laser doppler ⬆️
Ultrasound microbubble ⬇️
Ultrasound Med Biol 2024
www.sciencedirect.com/science/arti...
Contrast-enhanced ultrasound (whole calf) vs Laser Doppler (primarily skin) imaging of limb reperfusion post-ischemia
C57BL/6J🐭♂️ 10~12 wk
B/w 2- & 4-wk post-ischemia
Laser doppler ⬆️
Ultrasound microbubble ⬇️
Ultrasound Med Biol 2024
www.sciencedirect.com/science/arti...
Core-Shell droplet->deformable Angiogenic patch, + tunable stiffness to match different tissues
Shell: Alginate-ε-poly(L-lysine)
Core: VEGF:FGF:EGF:IGF 5:2:1:1
5 patches (quadriceps->gastroc) to treat🐭#LimbIschemia
ACS Appl Mater Interfaces 2023
pubs.acs.org/doi/10.1021/...
Shell: Alginate-ε-poly(L-lysine)
Core: VEGF:FGF:EGF:IGF 5:2:1:1
5 patches (quadriceps->gastroc) to treat🐭#LimbIschemia
ACS Appl Mater Interfaces 2023
pubs.acs.org/doi/10.1021/...
October 16, 2023 at 12:05 PM
Core-Shell droplet->deformable Angiogenic patch, + tunable stiffness to match different tissues
Shell: Alginate-ε-poly(L-lysine)
Core: VEGF:FGF:EGF:IGF 5:2:1:1
5 patches (quadriceps->gastroc) to treat🐭#LimbIschemia
ACS Appl Mater Interfaces 2023
pubs.acs.org/doi/10.1021/...
Shell: Alginate-ε-poly(L-lysine)
Core: VEGF:FGF:EGF:IGF 5:2:1:1
5 patches (quadriceps->gastroc) to treat🐭#LimbIschemia
ACS Appl Mater Interfaces 2023
pubs.acs.org/doi/10.1021/...
An important paper on Macrophage-#MuscleStemCell crosstalks in regenerative dynamics of ischemic #SkeletalMuscle #CriticalLimbThreateningIschemia
Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia
#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....
Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia
#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....
March 26, 2024 at 7:09 PM
An important paper on Macrophage-#MuscleStemCell crosstalks in regenerative dynamics of ischemic #SkeletalMuscle #CriticalLimbThreateningIschemia
Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia
#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....
Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia
#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....
Lipid-independent benefits of #PCSK9 #PeripheralArterialDisease #ChronicLimbThreateningIschemia
An interesting role of PCSK9 in hypoxia-mediated #Pyroptosis in #EndothelialCell & #Angiogenesis in mouse #LimbIschemia
Guifu Wu, Jianwen Liang labs #JACCBTS 2023
www.sciencedirect.com/science/arti...
An interesting role of PCSK9 in hypoxia-mediated #Pyroptosis in #EndothelialCell & #Angiogenesis in mouse #LimbIschemia
Guifu Wu, Jianwen Liang labs #JACCBTS 2023
www.sciencedirect.com/science/arti...
October 5, 2023 at 1:12 PM
Lipid-independent benefits of #PCSK9 #PeripheralArterialDisease #ChronicLimbThreateningIschemia
An interesting role of PCSK9 in hypoxia-mediated #Pyroptosis in #EndothelialCell & #Angiogenesis in mouse #LimbIschemia
Guifu Wu, Jianwen Liang labs #JACCBTS 2023
www.sciencedirect.com/science/arti...
An interesting role of PCSK9 in hypoxia-mediated #Pyroptosis in #EndothelialCell & #Angiogenesis in mouse #LimbIschemia
Guifu Wu, Jianwen Liang labs #JACCBTS 2023
www.sciencedirect.com/science/arti...
AteloCollagen 10%-#HyaluronicAcid 5% microgel as Cell Carrier of👤#AdiposeDerivedStemCell to treat🐭#LimbIschemia
⏫ADSC Survival in ischemic limb up to 28 days
⏫Material transfer (nutrient uptake+secretome release)
Which muscle studied?
#BioactiveMat 2023
www.sciencedirect.com/science/arti...
⏫ADSC Survival in ischemic limb up to 28 days
⏫Material transfer (nutrient uptake+secretome release)
Which muscle studied?
#BioactiveMat 2023
www.sciencedirect.com/science/arti...
March 12, 2024 at 7:14 PM
AteloCollagen 10%-#HyaluronicAcid 5% microgel as Cell Carrier of👤#AdiposeDerivedStemCell to treat🐭#LimbIschemia
⏫ADSC Survival in ischemic limb up to 28 days
⏫Material transfer (nutrient uptake+secretome release)
Which muscle studied?
#BioactiveMat 2023
www.sciencedirect.com/science/arti...
⏫ADSC Survival in ischemic limb up to 28 days
⏫Material transfer (nutrient uptake+secretome release)
Which muscle studied?
#BioactiveMat 2023
www.sciencedirect.com/science/arti...
📢 Online First!
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease #VascSky
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease #VascSky
July 24, 2025 at 9:55 PM
📢 Online First!
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease #VascSky
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease #VascSky
Condition Tie2+ Monocyte/Mφ to treat #LimbIschemia
siRNA HTATIP2⏫NRP1+Angpt1
Tie2+ Mφ from #ChronicLimbThreateningIschemia👤exhibits ⏬angiogenic+arteriogenic benefit in vitro & in🐭
Alberto Smith, Bijan Modarai labs #JCIInsight 2023
insight.jci.org/articles/vie...
siRNA HTATIP2⏫NRP1+Angpt1
Tie2+ Mφ from #ChronicLimbThreateningIschemia👤exhibits ⏬angiogenic+arteriogenic benefit in vitro & in🐭
Alberto Smith, Bijan Modarai labs #JCIInsight 2023
insight.jci.org/articles/vie...
March 15, 2024 at 7:08 PM
Condition Tie2+ Monocyte/Mφ to treat #LimbIschemia
siRNA HTATIP2⏫NRP1+Angpt1
Tie2+ Mφ from #ChronicLimbThreateningIschemia👤exhibits ⏬angiogenic+arteriogenic benefit in vitro & in🐭
Alberto Smith, Bijan Modarai labs #JCIInsight 2023
insight.jci.org/articles/vie...
siRNA HTATIP2⏫NRP1+Angpt1
Tie2+ Mφ from #ChronicLimbThreateningIschemia👤exhibits ⏬angiogenic+arteriogenic benefit in vitro & in🐭
Alberto Smith, Bijan Modarai labs #JCIInsight 2023
insight.jci.org/articles/vie...
Fibroblast-converted #SmoothMuscleCell to treat🐭#LimbIschemia
retroviral Myocardin+all-trans #RetinoicAcid
👉Survive up to 28 d in ischemic limb muscle
👉Assume peri-arterial or peri-capillary locations
👉Pro-angiogenic secretome
#Circ 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....
retroviral Myocardin+all-trans #RetinoicAcid
👉Survive up to 28 d in ischemic limb muscle
👉Assume peri-arterial or peri-capillary locations
👉Pro-angiogenic secretome
#Circ 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....
March 12, 2025 at 7:32 PM
Fibroblast-converted #SmoothMuscleCell to treat🐭#LimbIschemia
retroviral Myocardin+all-trans #RetinoicAcid
👉Survive up to 28 d in ischemic limb muscle
👉Assume peri-arterial or peri-capillary locations
👉Pro-angiogenic secretome
#Circ 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....
retroviral Myocardin+all-trans #RetinoicAcid
👉Survive up to 28 d in ischemic limb muscle
👉Assume peri-arterial or peri-capillary locations
👉Pro-angiogenic secretome
#Circ 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....
#CircadianRhythm #PeripheralArteryDisease
Perturbed #CircadianClock in #SkeletalMuscle (ACTA-rtTA-TRE-Cre::Bmall) impairs #Myogenesis (⏬myostatin, ⏫myofiber hypertrophy) & #Angiogenesis (Coupled by⏬VEGF-A) in🐭 #LimbIschemia
#ATVB 2024 @ahascience.bsky.social
www.ahajournals.org/doi/abs/10.1...
Perturbed #CircadianClock in #SkeletalMuscle (ACTA-rtTA-TRE-Cre::Bmall) impairs #Myogenesis (⏬myostatin, ⏫myofiber hypertrophy) & #Angiogenesis (Coupled by⏬VEGF-A) in🐭 #LimbIschemia
#ATVB 2024 @ahascience.bsky.social
www.ahajournals.org/doi/abs/10.1...
December 16, 2024 at 1:15 PM
#CircadianRhythm #PeripheralArteryDisease
Perturbed #CircadianClock in #SkeletalMuscle (ACTA-rtTA-TRE-Cre::Bmall) impairs #Myogenesis (⏬myostatin, ⏫myofiber hypertrophy) & #Angiogenesis (Coupled by⏬VEGF-A) in🐭 #LimbIschemia
#ATVB 2024 @ahascience.bsky.social
www.ahajournals.org/doi/abs/10.1...
Perturbed #CircadianClock in #SkeletalMuscle (ACTA-rtTA-TRE-Cre::Bmall) impairs #Myogenesis (⏬myostatin, ⏫myofiber hypertrophy) & #Angiogenesis (Coupled by⏬VEGF-A) in🐭 #LimbIschemia
#ATVB 2024 @ahascience.bsky.social
www.ahajournals.org/doi/abs/10.1...
Detailed planning of AAV9 intramuscular injection in ischemis🐭limb
AAV9-IGF1⏫myogenesis in🐭#LimbIschemia, w/o impacting satellite cell self-renewal or #Angiogenesis
Interesting myogenesis-angiogenesis decoupling!🤓
Terence Ryan lab #JACCBTS 2023
www.sciencedirect.com/science/arti...
AAV9-IGF1⏫myogenesis in🐭#LimbIschemia, w/o impacting satellite cell self-renewal or #Angiogenesis
Interesting myogenesis-angiogenesis decoupling!🤓
Terence Ryan lab #JACCBTS 2023
www.sciencedirect.com/science/arti...
January 8, 2024 at 8:21 PM
Detailed planning of AAV9 intramuscular injection in ischemis🐭limb
AAV9-IGF1⏫myogenesis in🐭#LimbIschemia, w/o impacting satellite cell self-renewal or #Angiogenesis
Interesting myogenesis-angiogenesis decoupling!🤓
Terence Ryan lab #JACCBTS 2023
www.sciencedirect.com/science/arti...
AAV9-IGF1⏫myogenesis in🐭#LimbIschemia, w/o impacting satellite cell self-renewal or #Angiogenesis
Interesting myogenesis-angiogenesis decoupling!🤓
Terence Ryan lab #JACCBTS 2023
www.sciencedirect.com/science/arti...
Shaking the flask or Vertical-Wheel Bioreactor of 3D #hiPSC spheroids▶️9-fold⏫#SmoothMuscleCell yield🤠
Therapeutic benefit of Intramuscular injection of hiPSC-SMC in NOD🐭#Limbischemia
Stem Cell Res Ther 2024
stemcellres.biomedcentral.com/articles/10....
Therapeutic benefit of Intramuscular injection of hiPSC-SMC in NOD🐭#Limbischemia
Stem Cell Res Ther 2024
stemcellres.biomedcentral.com/articles/10....
December 14, 2024 at 1:05 PM
Shaking the flask or Vertical-Wheel Bioreactor of 3D #hiPSC spheroids▶️9-fold⏫#SmoothMuscleCell yield🤠
Therapeutic benefit of Intramuscular injection of hiPSC-SMC in NOD🐭#Limbischemia
Stem Cell Res Ther 2024
stemcellres.biomedcentral.com/articles/10....
Therapeutic benefit of Intramuscular injection of hiPSC-SMC in NOD🐭#Limbischemia
Stem Cell Res Ther 2024
stemcellres.biomedcentral.com/articles/10....
#EndothelialCell #Mitochodria #LimbIschemia #EndMT
Thrilled to have played a small role in this exciting story😀
Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition
#ScienceAdv 2024
www.science.org/doi/10.1126/...
Thrilled to have played a small role in this exciting story😀
Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition
#ScienceAdv 2024
www.science.org/doi/10.1126/...
August 12, 2024 at 11:13 AM
#EndothelialCell #Mitochodria #LimbIschemia #EndMT
Thrilled to have played a small role in this exciting story😀
Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition
#ScienceAdv 2024
www.science.org/doi/10.1126/...
Thrilled to have played a small role in this exciting story😀
Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition
#ScienceAdv 2024
www.science.org/doi/10.1126/...